Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   CRD75_RS06755 Genome accession   NZ_CP023769
Coordinates   1325946..1327844 (-) Length   632 a.a.
NCBI ID   WP_002994648.1    Uniprot ID   A0A5S4TL28
Organism   Streptococcus pyogenes strain HarveyGAS     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 1320946..1332844
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CRD75_RS06730 (CRD75_06730) - 1321682..1322059 (-) 378 WP_002991882.1 S1 RNA-binding domain-containing protein -
  CRD75_RS06735 (CRD75_06735) - 1322059..1323459 (-) 1401 WP_021340868.1 bifunctional Cof-type HAD-IIB family hydrolase/peptidylprolyl isomerase -
  CRD75_RS06740 (CRD75_06740) - 1323496..1324137 (-) 642 WP_002983671.1 response regulator transcription factor -
  CRD75_RS06745 (CRD75_06745) - 1324130..1325134 (-) 1005 WP_002991890.1 sensor histidine kinase -
  CRD75_RS06750 (CRD75_06750) liaF 1325131..1325823 (-) 693 WP_011054833.1 cell wall-active antibiotics response protein LiaF -
  CRD75_RS06755 (CRD75_06755) pknB 1325946..1327844 (-) 1899 WP_002994648.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  CRD75_RS06760 (CRD75_06760) - 1327841..1328581 (-) 741 WP_002983660.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  CRD75_RS06765 (CRD75_06765) rsmB 1328619..1329941 (-) 1323 WP_030126543.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  CRD75_RS06770 (CRD75_06770) fmt 1329931..1330866 (-) 936 WP_011285049.1 methionyl-tRNA formyltransferase -

Sequence

Protein


Download         Length: 632 a.a.        Molecular weight: 68819.06 Da        Isoelectric Point: 7.2899

>NTDB_id=249846 CRD75_RS06755 WP_002994648.1 1325946..1327844(-) (pknB) [Streptococcus pyogenes strain HarveyGAS]
MIQIGKLFAGRYRILKSIGRGGMADVYLANDLILDNEDVAIKVLRTNYQTDQVAVARFQREARAMAELNHPNIVAIRDIG
EEDGQQFLVMEYVDGADLKRYIQNHAPLSNNEVVRIMEEVLSAMTLAHQKGIVHRDLKPQNILLTKEGVVKVTDFGIAVA
FAETSLTQTNSMLGSVHYLSPEQARGSKATIQSDIYAMGIMLFEMLTGHIPYDGDSAVTIALQHFQKPLPSIIEENHNVP
QALENVVIRATAKKLSDRYGSTFEMSRDLMTALSYNRSRERKIVFEDVESTKPLPKVASGPTASVKLSPPTPTVLTQESR
LDQTNQTDALQPPTKKKKSGRFLGTLFKILFSFFIVGVALFTYLILTKPTSVKVPNVAGTSLKVAKQELYDVGLKVGKIR
QIESDTVAEGNVVRTDPKAGTAKRQGSSITLYVSIGNKGFDMENYKGLDYQEAMNSLIETYGVPKSKIKIERIVTNEYPE
NTVISQSPSAGDKFNPNGKSKITLSVAVSDTITMPMVTEYSYADAVNTLTALGIDASRIKAYVPSSSSATGFVPIHSPSS
KAIVSGQSPYYGTSLSLSDKGEISLYLYPEETHSSSSSSSSTSSSNSSSTNDSTAPGSNTELSPSETTAQTP

Nucleotide


Download         Length: 1899 bp        

>NTDB_id=249846 CRD75_RS06755 WP_002994648.1 1325946..1327844(-) (pknB) [Streptococcus pyogenes strain HarveyGAS]
ATGATTCAGATTGGCAAATTATTTGCTGGTCGTTATCGCATTCTGAAATCTATTGGCCGCGGTGGTATGGCGGATGTTTA
TTTAGCAAATGATTTGATCTTGGATAATGAAGACGTTGCAATCAAGGTCTTGCGTACCAATTATCAAACAGATCAGGTAG
CAGTTGCGCGTTTCCAACGAGAAGCGCGGGCCATGGCTGAATTGAACCATCCCAATATTGTTGCCATCCGGGATATAGGT
GAAGAAGACGGACAGCAATTTTTAGTAATGGAATATGTGGATGGTGCTGACCTAAAGAGATACATTCAAAATCATGCTCC
ATTATCTAATAATGAAGTGGTTAGAATTATGGAAGAAGTCCTTTCTGCTATGACTTTAGCCCACCAAAAAGGAATTGTAC
ACAGAGATTTAAAACCTCAAAATATCCTACTAACTAAGGAGGGTGTTGTCAAAGTAACTGATTTCGGCATCGCAGTAGCC
TTTGCAGAAACAAGCTTGACACAAACTAATTCGATGTTAGGCAGTGTTCATTACTTGTCTCCAGAACAGGCTCGCGGCTC
CAAAGCGACGATTCAAAGTGATATTTATGCGATGGGGATTATGCTCTTTGAGATGTTGACAGGCCATATCCCTTATGATG
GCGATAGTGCTGTTACGATTGCCTTGCAACATTTTCAAAAGCCTCTTCCATCTATTATCGAGGAGAACCACAATGTGCCA
CAAGCTTTGGAGAATGTTGTTATTCGAGCAACAGCCAAGAAATTAAGTGATCGTTACGGGTCAACCTTTGAAATGAGTCG
TGACTTAATGACGGCGCTTAGTTATAATCGTAGTCGGGAGCGTAAGATTGTCTTTGAGGATGTTGAAAGTACCAAACCCC
TCCCCAAAGTGGCCTCAGGTCCCACCGCTTCTGTAAAATTGTCTCCCCCTACCCCAACAGTGTTAACACAGGAAAGTCGA
TTGGATCAAACTAATCAAACAGATGCTTTACAGCCCCCCACCAAAAAGAAAAAAAGTGGTCGTTTTTTAGGTACTTTATT
CAAAATTCTTTTTTCTTTCTTTATTGTAGGTGTAGCACTCTTTACTTATCTTATACTAACTAAACCAACTTCTGTGAAAG
TTCCTAATGTAGCAGGCACTAGTCTTAAAGTTGCCAAACAAGAACTGTATGATGTTGGGCTAAAAGTGGGTAAAATCAGG
CAAATTGAGAGTGATACGGTTGCTGAGGGAAATGTAGTTAGAACAGATCCTAAAGCAGGAACAGCTAAGAGGCAAGGCTC
AAGCATTACGCTTTATGTGTCAATTGGAAACAAAGGTTTTGACATGGAAAACTACAAAGGACTAGATTATCAAGAAGCTA
TGAATAGTTTGATAGAAACTTATGGTGTTCCAAAATCAAAAATCAAAATTGAGCGCATTGTAACTAATGAATATCCTGAA
AATACAGTCATCAGTCAATCGCCAAGTGCGGGTGATAAATTTAATCCAAACGGAAAGTCTAAAATTACGCTCAGTGTTGC
TGTTAGTGATACGATCACTATGCCTATGGTAACAGAATATAGTTATGCAGATGCAGTCAATACCTTAACAGCTTTAGGTA
TAGATGCATCTAGAATAAAAGCTTATGTGCCAAGCTCTAGCTCAGCAACGGGCTTTGTGCCAATTCATTCTCCTAGTTCT
AAAGCTATTGTCAGTGGTCAATCTCCTTACTATGGAACGTCTTTGAGTCTGTCTGATAAAGGAGAGATTAGTCTTTACCT
TTATCCAGAAGAAACACACTCTTCTAGTAGCTCATCGAGTTCAACGTCAAGTTCAAACAGTTCTTCAACAAATGATAGTA
CTGCACCAGGTAGCAACACTGAATTAAGCCCATCAGAAACTACTGCTCAAACACCTTAA

Domains

Predicted by InterproScan.

Protein kinase domain (Pkinase)

(12-242)

PASTA domain (PASTA)

(372-434)

PASTA domain (PASTA)

(441-506)

PASTA domain (PASTA)

(512-588)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A5S4TL28

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

65.424

93.354

0.611
  stkP/pknB Streptococcus salivarius strain HSISS4

61.513

94.146

0.579
  stkP Streptococcus pneumoniae TIGR4

57.171

81.646

0.467
  stkP Streptococcus pneumoniae D39

56.977

81.646

0.465
  stkP Streptococcus pneumoniae R6

56.977

81.646

0.465
  stkP Streptococcus pneumoniae Rx1

56.783

81.646

0.464

Multiple sequence alignment