Detailed information NTG0247049

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: CLI98_RS02135
• Genome accession: NZ_CP023431
• Coordinates: 422717..423331 (-)
• Length: 204 a.a.
• NCBI ID: WP_014418419.1
• Uniprot ID: I2C7Y9
• Organism: Bacillus velezensis strain SCGB 574
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 417717..428331

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CLI98_RS02110 (CLI98_00416)	rpsT	418020..418286 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
CLI98_RS02115 (CLI98_00417)	holA	418303..419344 (-)	1042	Protein_420	DNA polymerase III subunit delta	-
CLI98_RS19770	-	419384..419535 (-)	152	Protein_421	hypothetical protein	-
CLI98_RS02120	-	419576..419710 (+)	135	WP_003152870.1	YqzM family protein	-
CLI98_RS02125 (CLI98_00418)	comEC	419729..422080 (-)	2352	WP_022552933.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
CLI98_RS02130 (CLI98_00419)	-	422081..422650 (-)	570	WP_003152868.1	ComE operon protein 2	-
CLI98_RS02135 (CLI98_00420)	comEA	422717..423331 (-)	615	WP_014418419.1	helix-hairpin-helix domain-containing protein	Machinery gene
CLI98_RS02140 (CLI98_00421)	comER	423390..424211 (+)	822	WP_014418420.1	late competence protein ComER	-
CLI98_RS02145 (CLI98_00422)	-	424280..425017 (-)	738	WP_014418421.1	class I SAM-dependent methyltransferase	-
CLI98_RS02150 (CLI98_00423)	rsfS	425014..425370 (-)	357	WP_007408260.1	ribosome silencing factor	-
CLI98_RS02155 (CLI98_00424)	yqeK	425388..425948 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
CLI98_RS02160 (CLI98_00425)	-	425938..426507 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
CLI98_RS02165 (CLI98_00426)	yhbY	426518..426808 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
CLI98_RS02170 (CLI98_00427)	aroE	426802..427644 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22226.26 Da
• Isoelectric Point: 5.0355

>NTDB_id=247049 CLI98_RS02135 WP_014418419.1 422717..423331(-) (comEA) [Bacillus velezensis strain SCGB 574]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMEAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTTAGEKENAAVNINTASLDELQAVSGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=247049 CLI98_RS02135 WP_014418419.1 422717..423331(-) (comEA) [Bacillus velezensis strain SCGB 574]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCTGCAGGAGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGGAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAA
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGGTTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

3D structure

• AlphaFold DB: I2C7Y9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	36.019	100	0.373
comEA/celA/cilE	Streptococcus mitis SK321	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae D39	35.749	100	0.363
comEA/celA/cilE	Streptococcus pneumoniae R6	35.749	100	0.363