Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   CJD39_RS01725 Genome accession   NZ_CP023057
Coordinates   362384..362830 (-) Length   148 a.a.
NCBI ID   WP_111750327.1    Uniprot ID   -
Organism   Glaesserella sp. 15-184     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 357384..367830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CJD39_RS01700 (CJD39_01695) yacG 358229..358408 (-) 180 WP_111750322.1 DNA gyrase inhibitor YacG -
  CJD39_RS01705 (CJD39_01700) coaE 358405..359031 (-) 627 WP_111750323.1 dephospho-CoA kinase -
  CJD39_RS01710 (CJD39_01705) - 359058..359735 (-) 678 WP_111750324.1 prepilin peptidase -
  CJD39_RS01715 (CJD39_01710) pilC 359732..360928 (-) 1197 WP_111750325.1 type II secretion system F family protein Machinery gene
  CJD39_RS01720 (CJD39_01715) pilB 360921..362306 (-) 1386 WP_111750326.1 GspE/PulE family protein Machinery gene
  CJD39_RS01725 (CJD39_01720) pilA 362384..362830 (-) 447 WP_111750327.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CJD39_RS01730 (CJD39_01725) radA 363026..364405 (+) 1380 WP_111750328.1 DNA repair protein RadA -
  CJD39_RS01735 (CJD39_01730) ppc 364471..367110 (-) 2640 WP_111750329.1 phosphoenolpyruvate carboxylase -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15652.90 Da        Isoelectric Point: 8.7869

>NTDB_id=244633 CJD39_RS01725 WP_111750327.1 362384..362830(-) (pilA) [Glaesserella sp. 15-184]
MKNSRAIRPLAKAFTLIELMIVIAIIAILATIAIPSYTSYTQKAALSELLRASASYKSDVELCIYNTGALTNCTGGSNGI
QANKTDSTDTKYLKSVSVSAGVITVTGKGNIDGYGYTMTPKFANNNITWATSCQGSDTSLFPANFCGQ

Nucleotide


Download         Length: 447 bp        

>NTDB_id=244633 CJD39_RS01725 WP_111750327.1 362384..362830(-) (pilA) [Glaesserella sp. 15-184]
ATGAAAAATTCTAGAGCAATCCGACCGCTTGCAAAAGCATTTACCTTGATTGAGCTGATGATTGTGATCGCTATTATTGC
AATTTTAGCCACTATCGCCATTCCGTCTTATACCAGCTACACACAAAAAGCTGCCCTATCGGAATTATTGAGAGCGTCTG
CATCTTATAAATCCGATGTGGAACTCTGTATTTATAACACGGGAGCATTAACAAACTGTACTGGTGGTTCAAACGGCATT
CAAGCGAACAAAACTGATAGTACGGATACCAAATACCTGAAATCCGTTTCGGTATCCGCAGGTGTTATTACTGTCACAGG
TAAAGGAAACATTGACGGCTACGGTTACACAATGACACCGAAATTTGCCAATAACAACATTACTTGGGCAACTAGCTGTC
AAGGCTCTGATACAAGTTTGTTCCCTGCGAATTTCTGCGGACAGTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-144)

Prokaryotic N-terminal methylation motif (N_methyl)

(12-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

81.333

100

0.824
  pilA Haemophilus influenzae 86-028NP

61.702

95.27

0.588
  pilA Haemophilus influenzae Rd KW20

59.155

95.946

0.568
  comP Acinetobacter baylyi ADP1

46.032

85.135

0.392

Multiple sequence alignment