Detailed information NTG0241030

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: Sp14A_RS00765
• Genome accession: NZ_CP022601
• Coordinates: 125161..125598 (+)
• Length: 145 a.a.
• NCBI ID: WP_115129541.1
• Uniprot ID: A0A345VHA8
• Organism: Streptococcus pluranimalium strain 14A0014
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 120161..130598

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
Sp14A_RS00735 (Sp14A_01490)	-	121873..122253 (+)	381	WP_205407082.1	DUF1033 family protein	-
Sp14A_RS00740 (Sp14A_01500)	comYA	122316..123269 (+)	954	WP_115129537.1	competence type IV pilus ATPase ComGA	Machinery gene
Sp14A_RS00745 (Sp14A_01510)	comYB	123193..124221 (+)	1029	WP_115129538.1	competence type IV pilus assembly protein ComGB	Machinery gene
Sp14A_RS00750 (Sp14A_01520)	comYC	124222..124515 (+)	294	WP_115129539.1	competence type IV pilus major pilin ComGC	Machinery gene
Sp14A_RS00755 (Sp14A_01530)	comGD	124496..124912 (+)	417	WP_104967107.1	competence type IV pilus minor pilin ComGD	-
Sp14A_RS00760	comGE	124884..125183 (+)	300	WP_115129540.1	competence type IV pilus minor pilin ComGE	-
Sp14A_RS00765 (Sp14A_01540)	comYF	125161..125598 (+)	438	WP_115129541.1	competence type IV pilus minor pilin ComGF	Machinery gene
Sp14A_RS00770 (Sp14A_01550)	comGG	125582..125977 (+)	396	WP_205407083.1	competence type IV pilus minor pilin ComGG	-
Sp14A_RS00775 (Sp14A_01560)	comYH	126020..126973 (+)	954	WP_115129542.1	class I SAM-dependent methyltransferase	Machinery gene
Sp14A_RS00780 (Sp14A_01570)	-	127034..128224 (+)	1191	WP_115129543.1	acetate kinase	-
Sp14A_RS00785 (Sp14A_01580)	-	128609..128806 (+)	198	WP_115129544.1	helix-turn-helix transcriptional regulator	-
Sp14A_RS00790 (Sp14A_01590)	-	128819..129271 (+)	453	WP_115129545.1	hypothetical protein	-
Sp14A_RS00795 (Sp14A_01600)	-	129305..129976 (+)	672	WP_115129546.1	type II CAAX endopeptidase family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16835.51 Da
• Isoelectric Point: 9.7226

>NTDB_id=241030 Sp14A_RS00765 WP_115129541.1 125161..125598(+) (comYF) [Streptococcus pluranimalium strain 14A0014]
MLKHYHLKAFTLMECLLALLVLSGSIQVYQAMTKVVSTHVHELSQPKDQDWLLFCQQFRSELAGSQLVKVSDDRLVILKN
TQTLAFGKSKKEDFRKTSGDGRGFQPMIYNIESAHFRRFNQTISVQLIFKDGRVREFLYAFEKET

Nucleotide

• Length: 438 bp

>NTDB_id=241030 Sp14A_RS00765 WP_115129541.1 125161..125598(+) (comYF) [Streptococcus pluranimalium strain 14A0014]
GTGTTAAAACATTATCACCTTAAGGCATTCACTCTGATGGAGTGTTTGTTGGCATTACTGGTCCTATCGGGCTCCATCCA
GGTTTACCAAGCCATGACTAAGGTGGTTTCAACTCATGTTCATGAGCTCAGCCAACCTAAAGATCAGGACTGGTTGCTCT
TTTGCCAGCAATTTCGCTCAGAATTAGCAGGTTCTCAGTTGGTTAAAGTTTCTGATGATAGATTAGTCATCTTAAAAAAT
ACCCAAACCTTGGCTTTTGGTAAATCGAAAAAAGAGGATTTTCGCAAAACGAGTGGCGATGGTCGCGGCTTTCAACCTAT
GATTTATAACATAGAGTCAGCTCATTTTAGGCGGTTTAATCAGACCATCAGTGTTCAACTCATTTTTAAAGATGGAAGGG
TGCGTGAGTTTCTCTATGCTTTTGAAAAAGAGACTTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (37-134)

3D structure

• AlphaFold DB: A0A345VHA8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA159	55.172	100	0.552
comYF	Streptococcus mutans UA140	54.483	100	0.545
comGF/cglF	Streptococcus pneumoniae D39	42.958	97.931	0.421
comGF/cglF	Streptococcus pneumoniae Rx1	42.958	97.931	0.421
comGF/cglF	Streptococcus pneumoniae R6	42.958	97.931	0.421
comGF/cglF	Streptococcus pneumoniae TIGR4	42.958	97.931	0.421
comGF/cglF	Streptococcus mitis NCTC 12261	43.262	97.241	0.421
comGF/cglF	Streptococcus mitis SK321	40.426	97.241	0.393