Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CEK59_RS23375 Genome accession   NZ_CP022478
Coordinates   4931558..4932622 (-) Length   354 a.a.
NCBI ID   WP_058161054.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LW     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4926558..4937622
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK59_RS23350 (CEK59_23255) aroK 4927094..4927612 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CEK59_RS23355 (CEK59_23260) pilQ 4927624..4929768 (-) 2145 WP_058161055.1 type 4a pilus secretin PilQ Machinery gene
  CEK59_RS23360 (CEK59_23265) pilP 4929822..4930346 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  CEK59_RS23365 (CEK59_23270) pilO 4930343..4930965 (-) 623 Protein_4516 type 4a pilus biogenesis protein PilO -
  CEK59_RS23370 (CEK59_23275) pilN 4930962..4931558 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  CEK59_RS23375 (CEK59_23280) pilM 4931558..4932622 (-) 1065 WP_058161054.1 type IV pilus assembly protein PilM Machinery gene
  CEK59_RS23380 (CEK59_23285) - 4932807..4935278 (+) 2472 WP_023126737.1 penicillin-binding protein 1A -
  CEK59_RS23385 (CEK59_23290) - 4935374..4936642 (-) 1269 WP_003095842.1 malic enzyme -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37940.45 Da        Isoelectric Point: 4.4903

>NTDB_id=239953 CEK59_RS23375 WP_058161054.1 4931558..4932622(-) (pilM) [Pseudomonas aeruginosa strain LW]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPELVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=239953 CEK59_RS23375 WP_058161054.1 4931558..4932622(-) (pilM) [Pseudomonas aeruginosa strain LW]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGTCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAAGGGCTCTCCGCGC
GCAATCCCGAGCTCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAACTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GTACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTTTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.618

100

0.559
  comM Acinetobacter nosocomialis M2

55.618

100

0.559
  comM Acinetobacter baylyi ADP1

51.695

100

0.517
  pilM Legionella pneumophila strain ERS1305867

46.893

100

0.469

Multiple sequence alignment