Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CEK59_RS23355 Genome accession   NZ_CP022478
Coordinates   4927624..4929768 (-) Length   714 a.a.
NCBI ID   WP_058161055.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain LW     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4922624..4934768
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEK59_RS23340 (CEK59_23245) - 4924276..4925931 (-) 1656 WP_058161057.1 AAA family ATPase -
  CEK59_RS23345 (CEK59_23250) aroB 4925938..4927044 (-) 1107 WP_058161056.1 3-dehydroquinate synthase -
  CEK59_RS23350 (CEK59_23255) aroK 4927094..4927612 (-) 519 WP_003095833.1 shikimate kinase AroK -
  CEK59_RS23355 (CEK59_23260) pilQ 4927624..4929768 (-) 2145 WP_058161055.1 type 4a pilus secretin PilQ Machinery gene
  CEK59_RS23360 (CEK59_23265) pilP 4929822..4930346 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  CEK59_RS23365 (CEK59_23270) pilO 4930343..4930965 (-) 623 Protein_4516 type 4a pilus biogenesis protein PilO -
  CEK59_RS23370 (CEK59_23275) pilN 4930962..4931558 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN -
  CEK59_RS23375 (CEK59_23280) pilM 4931558..4932622 (-) 1065 WP_058161054.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77299.82 Da        Isoelectric Point: 5.2219

>NTDB_id=239952 CEK59_RS23355 WP_058161055.1 4927624..4929768(-) (pilQ) [Pseudomonas aeruginosa strain LW]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYATRVEGNNLFVVVGNSPAGASVASAAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGTSKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=239952 CEK59_RS23355 WP_058161055.1 4927624..4929768(-) (pilQ) [Pseudomonas aeruginosa strain LW]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCACTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTGCAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGCT
GTCGTCCTACGCCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCGCCGCTCCGGTCAAGGCAAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATCCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGCCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAACTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCTACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAACCGAGCGGGCTCTACGACTACCTGGTCTACCAGACCGACAATCGTTTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGCGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAACTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTGCTGGTGGCCCCGGCCGACGAGATTGCCGCCCGTGAGCGCCAGGAGTTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAATTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CGCGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGCCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AACGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGAACCAGTAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGATGCCACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTACCCTACCAGGAGGCCAGCTC
CAGCGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAAGTGAAGGTGACCAAGGATGCGCCGGACTTCGATCGCGCACTGAACGGCGTACCGCCGATCAACAAGAAC
GAGGTCAACGCCAAGATACTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGCAACACCCAGAGCAAGTC
GGTGGACAAGGTGCCGTTCCTCGGCGACCTGCCTTACCTGGGGCGACTGTTCCGGCGCGATACGGTCTCGGACGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Bacterial type II and III secretion system protein (Secretin)

(549-705)

AMIN domain (AMIN)

(38-121)

Secretin and TonB N terminus short domain (STN)

(307-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

97.899

100

0.979
  pilQ Acinetobacter baumannii strain A118

41.127

99.44

0.409
  pilQ Acinetobacter baumannii D1279779

41.127

99.44

0.409
  comQ Acinetobacter baylyi ADP1

41.394

98.459

0.408

Multiple sequence alignment