Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PBPR_RS15980 Genome accession   NC_006370
Coordinates   3577569..3578672 (-) Length   367 a.a.
NCBI ID   WP_081470364.1    Uniprot ID   -
Organism   Photobacterium profundum SS9     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3572569..3583672
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PBPR_RS15950 (PBPRA3136) - 3572931..3573674 (+) 744 WP_011219706.1 endonuclease -
  PBPR_RS15955 (PBPRA3137) rsmE 3573880..3574611 (+) 732 WP_011219707.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PBPR_RS15960 (PBPRA3138) gshB 3574641..3575588 (+) 948 WP_011219708.1 glutathione synthase -
  PBPR_RS15965 (PBPRA3139) - 3576186..3576749 (+) 564 WP_041394524.1 YqgE/AlgH family protein -
  PBPR_RS15970 (PBPRA3140) ruvX 3576746..3577174 (+) 429 WP_011219710.1 Holliday junction resolvase RuvX -
  PBPR_RS31135 - 3577321..3577500 (+) 180 WP_172635960.1 hypothetical protein -
  PBPR_RS15980 (PBPRA3141) pilU 3577569..3578672 (-) 1104 WP_081470364.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PBPR_RS15985 (PBPRA3142) pilT 3578747..3579784 (-) 1038 WP_011219712.1 type IV pilus twitching motility protein PilT Machinery gene
  PBPR_RS15990 (PBPRA3143) - 3579807..3580508 (+) 702 WP_041394526.1 YggS family pyridoxal phosphate-dependent enzyme -
  PBPR_RS15995 (PBPRA3144) proC 3580558..3581376 (+) 819 WP_011219714.1 pyrroline-5-carboxylate reductase -
  PBPR_RS16000 (PBPRA3145) - 3581386..3581941 (+) 556 Protein_3105 YggT family protein -
  PBPR_RS16005 (PBPRA3146) yggU 3581938..3582231 (+) 294 WP_041394527.1 DUF167 family protein YggU -
  PBPR_RS16010 (PBPRA3147) - 3582322..3582750 (+) 429 WP_011219717.1 DUF4426 domain-containing protein -

Sequence

Protein


Download         Length: 367 a.a.        Molecular weight: 41241.46 Da        Isoelectric Point: 5.7254

>NTDB_id=23888 PBPR_RS15980 WP_081470364.1 3577569..3578672(-) (pilU) [Photobacterium profundum SS9]
MKLQDILNEVVSKKASDLYITVDSPCLLRIDGHLHALGEELMREGVDALFDEAMDRELYQEFVTTREANFALVRGDMRFR
VSAFWQRELPGMVIRRIETHIPDLTDLNLPIDMQRMALAKRGLVLIVGATGSGKSTSMAAMIGFRNKNRSGHILTVEDPI
EFVHKHDRCIVTQREVGLDTESYEIALKNSLRQAPDVILIGEVRTPETMEYAMNFAETGHLCMATLHANNANQALERILH
LVPKERRDQFLFDLSLNLKCILAQQLIPDAHGIGRHATFELLLNTPRVADLIRRGELHEIKDVMAKSGESGMMTFDQSLY
QLFSEGKITEQDALHHADSANDLRLMIKIGDQAKMGGSALDGVTVEL

Nucleotide


Download         Length: 1104 bp        

>NTDB_id=23888 PBPR_RS15980 WP_081470364.1 3577569..3578672(-) (pilU) [Photobacterium profundum SS9]
ATGAAGCTGCAAGATATTCTCAATGAAGTCGTCAGCAAAAAAGCATCGGATTTATATATTACTGTTGATTCACCTTGTCT
GTTACGTATTGATGGTCACCTGCATGCATTAGGTGAAGAATTAATGCGTGAAGGGGTTGATGCATTGTTTGATGAAGCGA
TGGATCGGGAGTTATACCAAGAATTTGTCACGACACGTGAAGCCAATTTTGCATTAGTACGTGGTGATATGCGTTTTCGG
GTGAGTGCATTTTGGCAGCGTGAGTTGCCGGGCATGGTGATTCGTCGAATTGAAACCCACATCCCAGATTTGACTGATCT
CAATCTGCCCATTGATATGCAAAGAATGGCGTTAGCAAAGCGCGGCTTAGTGCTCATCGTAGGTGCGACAGGTTCGGGTA
AATCGACCTCGATGGCGGCAATGATTGGTTTTCGAAATAAAAACCGTAGCGGTCATATCTTAACGGTAGAAGATCCCATT
GAATTCGTGCATAAACATGATCGCTGTATCGTTACGCAGCGTGAAGTGGGCTTAGATACAGAGAGTTATGAGATTGCACT
AAAGAACTCGCTACGCCAAGCACCTGATGTGATCTTGATCGGTGAGGTACGTACACCTGAAACCATGGAGTATGCAATGA
ACTTTGCTGAAACCGGGCACCTGTGTATGGCAACGCTACATGCCAATAATGCCAATCAGGCACTGGAAAGAATTCTACAT
TTAGTCCCGAAAGAACGCCGTGATCAGTTTTTATTTGATCTGTCATTAAACCTGAAATGTATTTTGGCTCAGCAGTTGAT
CCCAGATGCACATGGTATTGGTCGGCATGCAACATTTGAATTGTTGCTGAATACGCCACGCGTGGCGGATCTTATTCGCC
GTGGTGAATTACATGAGATCAAAGATGTAATGGCGAAGTCAGGTGAATCCGGCATGATGACGTTTGATCAATCACTTTAT
CAATTGTTTAGTGAAGGAAAGATCACCGAGCAAGATGCACTCCATCATGCGGATTCTGCTAACGATCTACGCTTGATGAT
CAAAATTGGGGATCAAGCTAAGATGGGTGGTAGTGCGTTAGACGGTGTAACCGTTGAGTTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(112-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Vibrio cholerae strain A1552

68.665

100

0.687
  pilU Pseudomonas stutzeri DSM 10701

58.774

97.82

0.575
  pilU Acinetobacter baylyi ADP1

54.213

97.003

0.526
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.183

92.371

0.39

Multiple sequence alignment