NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	C8257_RS15370	Genome accession	NZ_CP028162
Coordinates	3220292..3220963 (-)	Length	223 a.a.
NCBI ID	WP_003090494.1	Uniprot ID	A0A0H2ZC88
Organism	Pseudomonas aeruginosa strain MRSN12280
Function	repress competence development (predicted from homology) Competence regulation

Genomic Context

Location: 3215292..3225963

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
C8257_RS34670	-	3216629..3217696 (+)	1068	Protein_2970	HAMP domain-containing protein	-
C8257_RS34675	-	3218018..3218554 (+)	537	Protein_2971	methyl-accepting chemotaxis protein	-
C8257_RS15360 (C8257_15365)	-	3218559..3218867 (-)	309	WP_003090492.1	hypothetical protein	-
C8257_RS15365 (C8257_15370)	carS	3218958..3220295 (-)	1338	WP_003090493.1	two-component system sensor histidine kinase CarS	-
C8257_RS15370 (C8257_15375)	ciaR	3220292..3220963 (-)	672	WP_003090494.1	two-component system response regulator CarR	Regulator
C8257_RS15375 (C8257_15380)	-	3220963..3221277 (-)	315	WP_003131079.1	PepSY domain-containing protein	-
C8257_RS15380 (C8257_15385)	-	3221277..3221585 (-)	309	WP_003090497.1	PepSY domain-containing protein	-
C8257_RS15385 (C8257_15390)	-	3221800..3222882 (+)	1083	WP_003090498.1	hypothetical protein	-
C8257_RS15390 (C8257_15395)	-	3222909..3223802 (+)	894	WP_003090499.1	hypothetical protein	-
C8257_RS15395 (C8257_15400)	-	3223786..3224052 (+)	267	WP_003090500.1	DUF5329 domain-containing protein	-
C8257_RS15400 (C8257_15405)	-	3224053..3225246 (-)	1194	WP_003090501.1	NnrS family protein	-
C8257_RS15405 (C8257_15410)	ppyR	3225233..3225490 (-)	258	WP_003090502.1	psl/pyoverdine operon transcriptional regulator PpyR	-

Sequence

Protein

Download Length: 223 a.a. Molecular weight: 24729.20 Da Isoelectric Point: 5.8445

                                >NTDB_id=238704 C8257_RS15370 WP_003090494.1 3220292..3220963(-) (ciaR) [Pseudomonas aeruginosa strain MRSN12280]

MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA

RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR

YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide

Download Length: 672 bp

                                >NTDB_id=238704 C8257_RS15370 WP_003090494.1 3220292..3220963(-) (ciaR) [Pseudomonas aeruginosa strain MRSN12280]

ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGACGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT

GGACTGGCTGGCCGACGGCCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGACCTGATCATCCTCGACCTCGGCC

TGCCCGGGCGCCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG

CGCGGTTCCTGGGCCGAACGCATCGATGGCCTCAAGGCCGGTGCCGACGACTACCTGACCAAGCCGTTCCATCCGGAAGA

GTTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCCGGGCTGC

GCCTGGACGAGCAGCGCCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC

TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA

TTCCAATGTCATCGAGGTCCACGTCAACCACTTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG

GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(147-216)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H2ZC88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus mutans UA159	37.727	98.655	0.372
ciaR	Streptococcus pneumoniae Rx1	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae D39	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae R6	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae TIGR4	37.273	98.655	0.368
micA	Streptococcus pneumoniae Cp1015	35.683	100	0.363
vicR	Streptococcus mutans UA159	35.526	100	0.363

Detailed information NTG0238704