Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   CFN12_RS08350 Genome accession   NZ_CP022290
Coordinates   1630860..1631357 (-) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain EDCC5458     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1625860..1636357
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN12_RS08325 (CFN12_08290) gcvPB 1626022..1627494 (-) 1473 WP_088607884.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -
  CFN12_RS08330 (CFN12_08295) gcvPA 1627487..1628833 (-) 1347 WP_000019687.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  CFN12_RS08335 (CFN12_08300) gcvT 1628853..1629944 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  CFN12_RS08340 (CFN12_08305) - 1630103..1630627 (-) 525 WP_100183654.1 shikimate kinase -
  CFN12_RS08345 (CFN12_08310) - 1630617..1630763 (-) 147 WP_001789879.1 hypothetical protein -
  CFN12_RS08350 (CFN12_08315) comGF 1630860..1631357 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  CFN12_RS08355 (CFN12_08320) comGE 1631275..1631574 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  CFN12_RS08360 (CFN12_08325) comGD 1631561..1632007 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  CFN12_RS08365 (CFN12_08330) comGC 1631985..1632296 (-) 312 WP_100183655.1 competence type IV pilus major pilin ComGC Machinery gene
  CFN12_RS08370 (CFN12_08335) comGB 1632310..1633380 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  CFN12_RS08375 (CFN12_08340) comGA 1633352..1634326 (-) 975 WP_054104768.1 competence type IV pilus ATPase ComGA Machinery gene
  CFN12_RS08380 (CFN12_08345) - 1634378..1635001 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  CFN12_RS08385 (CFN12_08350) - 1634998..1635327 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CFN12_RS08390 (CFN12_08355) - 1635327..1636313 (-) 987 WP_000161314.1 ROK family glucokinase -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=238050 CFN12_RS08350 WP_001789864.1 1630860..1631357(-) (comGF) [Staphylococcus aureus strain EDCC5458]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=238050 CFN12_RS08350 WP_001789864.1 1630860..1631357(-) (comGF) [Staphylococcus aureus strain EDCC5458]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment