Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   CFN12_RS08375 Genome accession   NZ_CP022290
Coordinates   1633352..1634326 (-) Length   324 a.a.
NCBI ID   WP_054104768.1    Uniprot ID   -
Organism   Staphylococcus aureus strain EDCC5458     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1628352..1639326
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CFN12_RS08335 (CFN12_08300) gcvT 1628853..1629944 (-) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  CFN12_RS08340 (CFN12_08305) - 1630103..1630627 (-) 525 WP_100183654.1 shikimate kinase -
  CFN12_RS08345 (CFN12_08310) - 1630617..1630763 (-) 147 WP_001789879.1 hypothetical protein -
  CFN12_RS08350 (CFN12_08315) comGF 1630860..1631357 (-) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  CFN12_RS08355 (CFN12_08320) comGE 1631275..1631574 (-) 300 WP_000844413.1 hypothetical protein Machinery gene
  CFN12_RS08360 (CFN12_08325) comGD 1631561..1632007 (-) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  CFN12_RS08365 (CFN12_08330) comGC 1631985..1632296 (-) 312 WP_100183655.1 competence type IV pilus major pilin ComGC Machinery gene
  CFN12_RS08370 (CFN12_08335) comGB 1632310..1633380 (-) 1071 WP_000775708.1 competence type IV pilus assembly protein ComGB Machinery gene
  CFN12_RS08375 (CFN12_08340) comGA 1633352..1634326 (-) 975 WP_054104768.1 competence type IV pilus ATPase ComGA Machinery gene
  CFN12_RS08380 (CFN12_08345) - 1634378..1635001 (-) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  CFN12_RS08385 (CFN12_08350) - 1634998..1635327 (-) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  CFN12_RS08390 (CFN12_08355) - 1635327..1636313 (-) 987 WP_000161314.1 ROK family glucokinase -
  CFN12_RS08395 (CFN12_08360) - 1636310..1636513 (-) 204 WP_000087561.1 YqgQ family protein -
  CFN12_RS08400 (CFN12_08365) - 1636494..1637957 (-) 1464 WP_001017867.1 rhomboid family intramembrane serine protease -
  CFN12_RS08405 (CFN12_08370) - 1637969..1638508 (-) 540 WP_000164545.1 5-formyltetrahydrofolate cyclo-ligase -
  CFN12_RS08410 (CFN12_08375) rpmG 1638717..1638866 (-) 150 WP_001265709.1 50S ribosomal protein L33 -

Sequence

Protein


Download         Length: 324 a.a.        Molecular weight: 36848.97 Da        Isoelectric Point: 9.0399

>NTDB_id=238055 CFN12_RS08375 WP_054104768.1 1633352..1634326(-) (comGA) [Staphylococcus aureus strain EDCC5458]
MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQIGNSIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYLF
NKIYFLRISTLPLSLGQESCVIRIVPQFFQPQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVIQASLSGHLVLTTLHATDCKGAI
LRPLEMGISVQELIQATNLIINQRLVTTIKQQRQLVCEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTM
HKYI

Nucleotide


Download         Length: 975 bp        

>NTDB_id=238055 CFN12_RS08375 WP_054104768.1 1633352..1634326(-) (comGA) [Staphylococcus aureus strain EDCC5458]
TTGAAGATTCTATTTCAAGAAATAATTAATAAAGCGATAGAAATGAAAGCGAGTGATGTACATTTTATTCCAGTTAAAAA
TGAAGTAAGTATTAAATTTAGAATTAATGATAACTTGGAGCAGTATGAACAAATTGGGAATAGCATTTATCAAAAGTTAT
TAGTTTATATGAAGTTTCAAGCTGGGCTTGATGTTTCTACACAGCAAGTCGCACAGAGCGGTCGATATAGTTACCTTTTC
AATAAAATATATTTTTTGAGAATATCAACTTTACCATTGTCACTTGGCCAAGAAAGTTGTGTTATCAGAATTGTACCTCA
ATTTTTTCAACCACAGAAATCAACTTATAAATTCAATGATTTTAAACACCTCATGAATAAGAAACAAGGATTACTATTGT
TTAGTGGACCAACTGGTTCAGGAAAGAGTACATTAATGTATCAAATGGTCTCATACGCGAATAAAGCCTTGAATTTAAAT
GTAATTTCTATAGAGGATCCTGTAGAGATGCAAATTCCTGGTATCGTCCAAATTAATGTGAATGATAAAGCTGGCATAAA
CTATGTAAATTCGTTTAAAGCTATTTTAAGATGTGATCCTGATGTTATTTTAATAGGTGAAATCAGAGATAAAGATGTTG
CCAAGTGTGTTATACAGGCTAGTTTAAGTGGTCACCTTGTTCTGACTACATTGCATGCAACTGATTGTAAAGGTGCTATT
TTAAGGCCATTAGAAATGGGCATTTCTGTACAAGAATTGATACAGGCAACTAACTTAATTATAAACCAACGACTTGTAAC
TACTATTAAGCAACAGCGACAATTAGTATGTGAAATTCTATCTCAGCAACAACTCCGATATTTCTTTTCCCATAATCATT
CATTACCATCATCATTTAAGAACTTAGAAGATAAACTTGATGATATGACAAAAGCAGGTGTCATTTGTGAAACTACAATG
CATAAATACATTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

98.765

100

0.988
  comGA Staphylococcus aureus N315

98.765

100

0.988

Multiple sequence alignment