Detailed information    

insolico Bioinformatically predicted

Overview

Name   comE   Type   Machinery gene
Locus tag   MS_RS09000 Genome accession   NC_006300
Coordinates   1941516..1942901 (-) Length   461 a.a.
NCBI ID   WP_041640034.1    Uniprot ID   -
Organism   [Mannheimia] succiniciproducens MBEL55E     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1936516..1947901
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  MS_RS08970 (MS1964) - 1936753..1937475 (+) 723 WP_041640030.1 3-deoxy-D-manno-octulosonic acid kinase -
  MS_RS08975 (MS1965) aqpZ 1937510..1938202 (-) 693 WP_011201123.1 aquaporin Z -
  MS_RS08980 (MS1966) metJ 1938431..1938748 (+) 318 WP_041640032.1 met regulon transcriptional regulator MetJ -
  MS_RS08985 (MS1967) - 1938819..1939664 (-) 846 WP_011201125.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  MS_RS08990 (MS1968) aroB 1939677..1940765 (-) 1089 WP_011201126.1 3-dehydroquinate synthase -
  MS_RS08995 (MS1969) aroK 1940795..1941322 (-) 528 WP_011201127.1 shikimate kinase AroK -
  MS_RS09000 (MS1970) comE 1941516..1942901 (-) 1386 WP_041640034.1 type IV pilus secretin PilQ Machinery gene
  MS_RS09005 (MS1971) - 1942916..1943326 (-) 411 WP_011201129.1 pilus assembly protein PilP -
  MS_RS09010 (MS1972) - 1943329..1943841 (-) 513 WP_041640036.1 hypothetical protein -
  MS_RS10900 (MS1973) - 1943819..1944355 (-) 537 WP_011201131.1 hypothetical protein -
  MS_RS09020 (MS1974) - 1944357..1945184 (-) 828 WP_011201132.1 hypothetical protein -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 50766.10 Da        Isoelectric Point: 6.7339

>NTDB_id=23677 MS_RS09000 WP_041640034.1 1941516..1942901(-) (comE) [[Mannheimia] succiniciproducens MBEL55E]
MESIISFGKKCGLFFGIFISSAFAGESGTFAERQFSIHLKKAPLVATLQQLALEQNANLVIDDELEGTLSLKLEKVNLER
LFHSVAKLKNLSLHKDKDIYYFTKNNLIEPSSIAGELKNTENFTALSEPNLVSTTVKLHFAKASEVMKSLTSGTGSLLSP
VGSVSFDERSNQLLIQDERRSLQNIKNIIAQLDKPIEQIAIEARIVTMNDESLKELGVRWGLLEGVNSAHRIAGSLEANG
FADIGQNLNVNFPTSATPAGSVALQVAKIHGRLLDLELTALEQENNVKIIASPRLLTTNKKSASIKQGTEIPYVAVNRKN
DTEHVEFREAVLGLEVTPHISKDNSILLDLIVSQNSPGANIVYGNGNLISIDKQEINTQVFAKDGETIVLGGVFNDTITK
SEDKVPILGDIPLIKHLFSKESEKHQKRELVIFVTPHILKQGESLEQIQKRFKYAPKSPEK

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=23677 MS_RS09000 WP_041640034.1 1941516..1942901(-) (comE) [[Mannheimia] succiniciproducens MBEL55E]
ATGGAAAGCATAATAAGTTTTGGTAAAAAGTGCGGTCTGTTTTTTGGAATTTTTATTTCTTCCGCATTTGCCGGGGAATC
CGGTACGTTTGCGGAACGGCAATTTTCCATTCACTTGAAAAAGGCGCCGTTGGTGGCGACCTTACAACAGCTTGCTTTAG
AACAAAATGCTAATCTGGTGATTGATGACGAACTGGAAGGTACATTGTCGTTAAAGCTTGAAAAGGTGAATCTGGAGCGT
CTTTTTCATTCGGTGGCAAAGCTGAAAAATCTTAGCTTACATAAAGACAAGGATATTTATTATTTTACGAAAAATAACCT
GATTGAACCGTCTTCCATCGCCGGTGAGCTAAAAAATACGGAAAATTTTACCGCACTTTCGGAACCTAATCTGGTTAGTA
CTACCGTCAAACTGCATTTTGCCAAAGCCTCGGAGGTGATGAAATCATTAACATCGGGAACGGGTTCATTGCTTTCGCCC
GTTGGTTCCGTCAGCTTTGATGAACGCAGTAACCAGCTGCTTATTCAAGATGAACGCAGATCGTTGCAAAATATCAAAAA
TATAATCGCTCAATTAGATAAACCTATCGAACAAATCGCAATTGAAGCCCGTATCGTGACGATGAACGACGAAAGTTTAA
AAGAACTCGGTGTGCGTTGGGGGCTGTTGGAGGGGGTAAATAGCGCACATCGTATTGCCGGCAGCCTGGAAGCTAACGGA
TTCGCCGATATAGGGCAAAACCTTAATGTTAATTTCCCGACCAGTGCGACACCGGCAGGTTCCGTAGCGTTGCAGGTGGC
AAAAATTCACGGGCGTTTGCTGGATTTGGAATTAACCGCTTTAGAACAGGAGAACAATGTCAAAATCATTGCCAGCCCGC
GGCTGCTGACTACCAATAAGAAAAGTGCGAGTATTAAACAAGGAACGGAAATTCCTTATGTGGCGGTGAATCGTAAAAAT
GATACGGAACACGTGGAGTTTCGAGAGGCGGTGCTAGGGTTGGAAGTTACACCGCATATTTCAAAAGATAATTCTATTTT
ACTTGACCTTATCGTAAGCCAGAATTCTCCGGGCGCAAATATTGTTTATGGGAATGGCAATTTAATTTCCATTGATAAAC
AGGAAATTAATACGCAGGTTTTTGCAAAAGACGGCGAAACCATCGTATTAGGCGGCGTATTTAACGATACTATAACGAAA
AGCGAAGATAAAGTGCCGATTTTGGGCGATATTCCGCTGATAAAACATTTATTCAGCAAAGAAAGCGAAAAACACCAAAA
ACGGGAATTGGTTATTTTTGTTACGCCTCATATATTAAAACAAGGCGAAAGCTTGGAGCAAATACAAAAACGTTTTAAAT
ATGCGCCAAAATCTCCTGAAAAATAG

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(280-440)

Bacterial type II/III secretion system short domain (Secretin_N)

(135-198)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comE Haemophilus influenzae Rd KW20

66.742

95.879

0.64
  comE Haemophilus influenzae 86-028NP

65.837

95.879

0.631
  comE Glaesserella parasuis strain SC1401

51.869

92.842

0.482
  pilQ Vibrio campbellii strain DS40M4

40.984

92.625

0.38
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.281

92.625

0.373
  pilQ Vibrio cholerae strain A1552

40.281

92.625

0.373

Multiple sequence alignment