Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEP49_RS04645 Genome accession   NZ_CP022012
Coordinates   1045844..1047262 (-) Length   472 a.a.
NCBI ID   WP_265473582.1    Uniprot ID   -
Organism   Mergibacter septicus strain 16309-1-I1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1040844..1052262
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP49_RS04615 (CEP49_04590) - 1040914..1042314 (-) 1401 WP_265473577.1 sodium:alanine symporter family protein -
  CEP49_RS04620 (CEP49_04595) - 1042684..1042956 (-) 273 WP_265475097.1 GNAT family N-acetyltransferase -
  CEP49_RS04625 (CEP49_04600) yacG 1042996..1043202 (-) 207 WP_265473578.1 DNA gyrase inhibitor YacG -
  CEP49_RS04630 (CEP49_04605) coaE 1043204..1043833 (-) 630 WP_265473579.1 dephospho-CoA kinase -
  CEP49_RS04635 (CEP49_04610) - 1043872..1044561 (-) 690 WP_265473580.1 prepilin peptidase -
  CEP49_RS04640 (CEP49_04615) - 1044603..1045829 (-) 1227 WP_265473581.1 type II secretion system F family protein -
  CEP49_RS04645 (CEP49_04620) pilB 1045844..1047262 (-) 1419 WP_265473582.1 GspE/PulE family protein Machinery gene
  CEP49_RS04650 (CEP49_04625) pilA 1047284..1047751 (-) 468 WP_265473583.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CEP49_RS04655 (CEP49_04630) ampD 1047879..1048478 (+) 600 WP_409240660.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CEP49_RS04660 (CEP49_04635) - 1048643..1049710 (+) 1068 WP_265473584.1 LysM-like peptidoglycan-binding domain-containing protein -
  CEP49_RS04665 (CEP49_04640) cgtA 1049774..1050934 (-) 1161 WP_265473585.1 Obg family GTPase CgtA -
  CEP49_RS04670 (CEP49_04645) - 1050978..1051910 (-) 933 WP_265473586.1 DMT family transporter -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 53253.90 Da        Isoelectric Point: 5.0670

>NTDB_id=235866 CEP49_RS04645 WP_265473582.1 1045844..1047262(-) (pilB) [Mergibacter septicus strain 16309-1-I1]
MLYSVFEQDSQRVFTISTELWQQNQQKYPLLLRYLAVPVQEDSTTLWLAIESLNNIRGCETFSFLTGKIVEPVVIGQFGL
KQLLQALNPQNDAIYAVAKENTLYEPQSDYLSDSVELDPDEPMIKLFNQLLEQSIQHRASDIHLEPSAESYLVRFRIDGI
LQLQQQFNFSLGGRLLSRLKLLAKLDISETRLPQDGHFSFKTTFAETLELRLSTLPTQFGEKMVLRLQHNQPTTLNFTEL
GMTAAQVEMFKTALLQPQGLILVTGPTGSGKSLTLYSGLSFLNSEDKHLMTAEDPIEIPLAGVIQTQVNNPIGLTFVKLL
RTFLRQDPDVIMVGEIRDQETAEMALRAAQTGHLVLSTLHTNTARSAITRLQQLGIQQSELENTLLLVIAQRLLRKKCQN
CCHLSQTACQCTNGYTGRIGVYQFLVRQTVNQQESFISDYDSLEQAALPLLEKGITDQAEVDRVLGTQQFHY

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=235866 CEP49_RS04645 WP_265473582.1 1045844..1047262(-) (pilB) [Mergibacter septicus strain 16309-1-I1]
ATGTTGTATAGCGTGTTTGAGCAAGATAGCCAACGGGTCTTTACGATATCGACTGAGTTATGGCAACAGAACCAACAAAA
ATACCCATTATTACTGCGTTATTTAGCTGTACCAGTGCAAGAAGACTCAACGACTTTATGGTTAGCTATTGAATCGTTGA
ATAATATTCGTGGCTGCGAAACTTTTTCTTTTTTAACTGGAAAAATCGTTGAACCCGTAGTAATTGGGCAATTTGGTTTA
AAACAGTTATTGCAAGCATTAAATCCACAGAATGATGCAATTTATGCTGTGGCTAAGGAAAATACTTTATATGAACCACA
AAGTGATTATTTATCTGATAGTGTGGAATTAGATCCAGATGAACCAATGATCAAGTTATTTAATCAATTGTTAGAACAGA
GTATTCAACACAGAGCATCTGATATTCATCTTGAACCAAGTGCAGAAAGTTATCTAGTACGTTTTCGGATTGATGGTATT
TTGCAGTTACAACAGCAGTTTAACTTTTCTTTAGGCGGACGATTGCTGTCTCGTTTAAAGCTATTAGCGAAGTTAGATAT
TAGTGAAACACGTTTACCACAAGATGGGCATTTTTCTTTTAAAACCACTTTTGCTGAAACATTAGAATTACGTCTTTCCA
CCTTACCGACCCAATTTGGTGAAAAAATGGTATTACGTTTACAGCATAATCAACCTACTACATTGAATTTTACTGAGTTA
GGTATGACGGCAGCACAAGTTGAGATGTTTAAAACGGCATTGTTACAACCACAAGGCTTAATTTTAGTAACAGGACCAAC
TGGTAGTGGTAAAAGTTTAACTTTATACAGTGGATTAAGCTTTTTAAATTCTGAAGATAAACATTTAATGACGGCTGAAG
ATCCGATTGAAATTCCATTAGCAGGCGTAATTCAAACACAGGTAAATAATCCTATTGGATTAACCTTTGTAAAGTTATTG
CGTACCTTTTTACGGCAAGATCCTGATGTCATTATGGTTGGAGAAATTCGAGATCAAGAAACCGCTGAAATGGCATTAAG
AGCAGCACAAACAGGGCATTTAGTTTTATCGACCTTGCATACTAATACCGCCAGATCGGCGATCACACGTTTACAGCAAT
TAGGTATTCAACAAAGTGAGTTAGAAAATACGTTGTTGCTAGTAATTGCTCAACGCTTACTGCGAAAAAAATGTCAAAAT
TGTTGTCACTTATCGCAAACAGCTTGTCAGTGTACTAATGGTTATACTGGGCGAATAGGGGTATATCAATTTTTAGTTCG
CCAAACTGTTAATCAACAAGAGTCGTTTATCAGTGATTATGACAGCCTTGAACAAGCTGCACTTCCTTTATTGGAAAAAG
GCATTACTGATCAGGCAGAAGTCGATCGAGTATTAGGCACTCAACAATTTCATTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae Rd KW20

57.709

96.186

0.555
  pilB Haemophilus influenzae 86-028NP

57.489

96.186

0.553
  pilB Glaesserella parasuis strain SC1401

53.747

98.941

0.532
  pilB Vibrio parahaemolyticus RIMD 2210633

40.417

100

0.411
  pilB Vibrio campbellii strain DS40M4

39.918

100

0.411
  pilB Vibrio cholerae strain A1552

39.256

100

0.403
  pilB Acinetobacter baylyi ADP1

37.6

100

0.398
  pilB Legionella pneumophila strain ERS1305867

37.172

100

0.39
  pilB Acinetobacter baumannii D1279779

37.143

100

0.386

Multiple sequence alignment