Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CEP45_RS05625 Genome accession   NZ_CP022010
Coordinates   1245819..1247237 (-) Length   472 a.a.
NCBI ID   WP_265490391.1    Uniprot ID   -
Organism   Mergibacter septicus strain 27643-20-I1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1240819..1252237
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEP45_RS05595 (CEP45_05580) - 1240890..1242290 (-) 1401 WP_265490387.1 sodium:alanine symporter family protein -
  CEP45_RS05600 (CEP45_05585) - 1242660..1242932 (-) 273 WP_261920967.1 GNAT family N-acetyltransferase -
  CEP45_RS05605 (CEP45_05590) yacG 1242972..1243178 (-) 207 WP_265490388.1 DNA gyrase inhibitor YacG -
  CEP45_RS05610 (CEP45_05595) coaE 1243171..1243809 (-) 639 WP_261920347.1 dephospho-CoA kinase -
  CEP45_RS05615 (CEP45_05600) - 1243848..1244537 (-) 690 WP_265490389.1 prepilin peptidase -
  CEP45_RS05620 (CEP45_05605) - 1244578..1245804 (-) 1227 WP_265490390.1 type II secretion system F family protein -
  CEP45_RS05625 (CEP45_05610) pilB 1245819..1247237 (-) 1419 WP_265490391.1 GspE/PulE family protein Machinery gene
  CEP45_RS05630 (CEP45_05615) pilA 1247259..1247726 (-) 468 WP_261920343.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  CEP45_RS05635 (CEP45_05620) ampD 1247854..1248453 (+) 600 WP_409240816.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  CEP45_RS05640 (CEP45_05625) - 1248617..1249684 (+) 1068 WP_265490392.1 LysM-like peptidoglycan-binding domain-containing protein -
  CEP45_RS05645 (CEP45_05630) cgtA 1249748..1250908 (-) 1161 WP_265490393.1 Obg family GTPase CgtA -
  CEP45_RS05650 (CEP45_05635) - 1250952..1251884 (-) 933 WP_265490394.1 DMT family transporter -

Sequence

Protein


Download         Length: 472 a.a.        Molecular weight: 53179.82 Da        Isoelectric Point: 5.0670

>NTDB_id=235827 CEP45_RS05625 WP_265490391.1 1245819..1247237(-) (pilB) [Mergibacter septicus strain 27643-20-I1]
MLYSVFEQDSQRVFTISTELWQQNQQKYPLLLRYLAVPVQEDSTTLWLAIESLNNIRGCETFSFLTGKIVEPVVIGQFGL
KQLLQALNPQNDAIYAVAKENTLYEPQSDYLSDSVELDPDEPMIKLFNQLLEQSIQHRASDIHLEPSAESYLVRFRIDGI
LQLQQQFNFSLGGRLLSRLKLLAKLDISETRLPQDGHFSFKTTFAETLELRLSTLPTQFGEKMVLRLQHNQPTTLNFAEL
GMTAAQVEMFKTALLQPQGLILVTGPTGSGKSLTLYSGLSFLNAEDKHLMTAEDPIEIPLAGVIQTQVNNPIGLTFAKLL
RTFLRQDPDVIMVGEIRDQETAEMALRAAQTGHLVLSTLHTNTARSAITRLQQLGIQQSELENTLLLVIAQRLLRKKCQN
CCHLSQTACQCTNGYTGRIGVYQFLVRQTVNQQESFISDYDSLEQAALPLLEKGITDQAEVDRVLGTQQFHY

Nucleotide


Download         Length: 1419 bp        

>NTDB_id=235827 CEP45_RS05625 WP_265490391.1 1245819..1247237(-) (pilB) [Mergibacter septicus strain 27643-20-I1]
ATGTTGTATAGCGTGTTTGAACAAGATAGCCAGCGGGTATTTACGATATCGACTGAGTTATGGCAACAGAACCAACAAAA
ATACCCATTATTACTGCGTTATTTAGCTGTACCAGTGCAAGAAGACTCAACGACTTTATGGTTAGCTATTGAATCGTTGA
ATAATATTCGTGGCTGCGAAACCTTTTCTTTTTTAACTGGAAAAATCGTTGAACCCGTAGTAATTGGGCAATTTGGTTTA
AAACAGTTATTGCAAGCATTAAATCCACAGAATGATGCAATTTATGCTGTGGCTAAGGAAAATACTTTATATGAACCACA
AAGTGATTATTTATCTGATAGTGTGGAATTAGATCCAGATGAACCAATGATTAAGTTATTTAATCAGTTGTTAGAACAGA
GTATTCAACACAGAGCATCTGATATTCATCTTGAACCAAGTGCAGAAAGTTATCTAGTACGTTTTCGGATTGATGGTATT
TTGCAGTTACAACAGCAGTTTAACTTTTCTTTGGGCGGGCGATTGCTATCTCGTCTAAAGCTATTAGCGAAGTTAGATAT
TAGTGAAACACGTTTACCACAAGATGGGCATTTTTCTTTTAAAACCACTTTTGCTGAAACCTTAGAATTACGTCTTTCCA
CCTTACCGACTCAATTTGGCGAAAAAATGGTATTACGTTTACAGCATAATCAACCTACTACATTGAATTTTGCTGAGTTA
GGTATGACGGCAGCACAAGTTGAGATGTTTAAAACTGCATTGTTACAACCACAAGGCTTAATTTTAGTAACAGGACCAAC
TGGTAGTGGTAAAAGTTTAACTTTATACAGTGGGTTAAGCTTTTTAAATGCTGAAGATAAACATTTAATGACGGCGGAAG
ATCCGATTGAAATTCCATTAGCAGGCGTAATTCAAACACAGGTAAATAATCCTATTGGATTAACCTTTGCAAAGTTATTG
CGTACCTTTTTACGGCAAGATCCTGATGTCATTATGGTTGGAGAAATTCGAGATCAAGAAACCGCTGAAATGGCATTAAG
AGCAGCACAAACAGGGCATTTAGTTTTATCGACCTTGCATACTAATACTGCCAGATCGGCGATCACACGTTTACAGCAGT
TAGGTATTCAACAAAGTGAGTTAGAAAATACGTTGTTGCTAGTAATTGCTCAACGCTTACTGCGAAAAAAATGTCAAAAT
TGTTGTCATTTATCGCAAACAGCTTGTCAGTGTACTAATGGTTATACTGGACGAATAGGGGTATATCAATTTTTAGTTCG
CCAAACTGTTAATCAACAAGAGTCGTTTATCAGTGATTATGACAGTCTCGAACAAGCTGCACTTCCTTTATTGGAAAAAG
GCATTACTGATCAGGCAGAAGTCGATCGAGTATTAGGCACTCAACAATTTCATTACTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(129-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

57.709

96.186

0.555
  pilB Haemophilus influenzae Rd KW20

57.709

96.186

0.555
  pilB Glaesserella parasuis strain SC1401

53.747

98.941

0.532
  pilB Vibrio parahaemolyticus RIMD 2210633

40.625

100

0.413
  pilB Vibrio campbellii strain DS40M4

40.123

100

0.413
  pilB Vibrio cholerae strain A1552

39.463

100

0.405
  pilB Acinetobacter baylyi ADP1

37.8

100

0.4
  pilB Legionella pneumophila strain ERS1305867

37.172

100

0.39
  pilB Acinetobacter baumannii D1279779

37.347

100

0.388

Multiple sequence alignment