Detailed information NTG0235151

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: AM474_RS17660
• Genome accession: NZ_CP027538
• Coordinates: 3638906..3639970 (+)
• Length: 354 a.a.
• NCBI ID: WP_003110888.1
• Uniprot ID: A0A0H2ZID6
• Organism: Pseudomonas aeruginosa strain AR_0095
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3633906..3644970

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM474_RS17650 (AM474_17650)	-	3634886..3636154 (+)	1269	WP_003095842.1	malic enzyme	-
AM474_RS17655 (AM474_17655)	-	3636250..3638721 (-)	2472	WP_003095841.1	penicillin-binding protein 1A	-
AM474_RS17660 (AM474_17660)	pilM	3638906..3639970 (+)	1065	WP_003110888.1	type IV pilus assembly protein PilM	Machinery gene
AM474_RS17665 (AM474_17665)	pilN	3639970..3640566 (+)	597	WP_003141783.1	type 4a pilus biogenesis protein PilN	-
AM474_RS17670 (AM474_17670)	pilO	3640563..3641186 (+)	624	WP_003116135.1	type 4a pilus biogenesis protein PilO	Machinery gene
AM474_RS17675 (AM474_17675)	comL/pilP	3641183..3641707 (+)	525	WP_003116136.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
AM474_RS17680 (AM474_17680)	pilQ	3641761..3643536 (+)	1776	Protein_3431	type IV pilus secretin PilQ	-
AM474_RS17685 (AM474_17685)	-	3643625..3643849 (-)	225	WP_003117186.1	DUF7364 domain-containing protein	-
AM474_RS17690 (AM474_17690)	-	3643928..3644215 (-)	288	WP_033965861.1	hypothetical protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37983.48 Da
• Isoelectric Point: 4.5405

>NTDB_id=235151 AM474_RS17660 WP_003110888.1 3638906..3639970(+) (pilM) [Pseudomonas aeruginosa strain AR_0095]
MLGLIKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVV
AVAGSAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANP
FADMALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=235151 AM474_RS17660 WP_003110888.1 3638906..3639970(+) (pilM) [Pseudomonas aeruginosa strain AR_0095]
GTGCTAGGGCTCATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTT
GAGCCGCTCCGGAGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACA
TCGTCGAGCTGGAAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTC
GCCGTCGCCGGTTCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCT
GAAGATCGAGGCCGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGC
GCAATCCCGAGCGCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTG
GCCGGCCTGACCGCCAAGGTGGTCGACGTCGAGGCTTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGTTGGG
TGCCGACACCGACCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGAC
GCACCATCTATACCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTG
GAGGAAGCCGGTCTCGCCAAGAAGCAGGGTGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGC
CGTGGTGCAGCAGGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGG
CGGGCGGTACGGCGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCG
TTCGCCGACATGGCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGG
CCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A0H2ZID6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.899	100	0.562
comM	Acinetobacter nosocomialis M2	55.899	100	0.562
comM/pilM	Acinetobacter baylyi ADP1	51.977	100	0.52
pilM	Legionella pneumophila strain ERS1305867	46.61	100	0.466