Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   AM474_RS17670 Genome accession   NZ_CP027538
Coordinates   3640563..3641186 (+) Length   207 a.a.
NCBI ID   WP_003116135.1    Uniprot ID   A0A0H2ZIF6
Organism   Pseudomonas aeruginosa strain AR_0095     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3635563..3646186
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AM474_RS17655 (AM474_17655) - 3636250..3638721 (-) 2472 WP_003095841.1 penicillin-binding protein 1A -
  AM474_RS17660 (AM474_17660) pilM 3638906..3639970 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  AM474_RS17665 (AM474_17665) pilN 3639970..3640566 (+) 597 WP_003141783.1 type 4a pilus biogenesis protein PilN -
  AM474_RS17670 (AM474_17670) pilO 3640563..3641186 (+) 624 WP_003116135.1 type 4a pilus biogenesis protein PilO Machinery gene
  AM474_RS17675 (AM474_17675) comL/pilP 3641183..3641707 (+) 525 WP_003116136.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  AM474_RS17680 (AM474_17680) pilQ 3641761..3643536 (+) 1776 Protein_3431 type IV pilus secretin PilQ -
  AM474_RS17685 (AM474_17685) - 3643625..3643849 (-) 225 WP_003117186.1 DUF7364 domain-containing protein -
  AM474_RS17690 (AM474_17690) - 3643928..3644215 (-) 288 WP_033965861.1 hypothetical protein -
  AM474_RS17695 (AM474_17695) - 3644212..3645360 (-) 1149 WP_033965860.1 hypothetical protein -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22958.35 Da        Isoelectric Point: 4.9107

>NTDB_id=235152 AM474_RS17670 WP_003116135.1 3640563..3641186(+) (pilO) [Pseudomonas aeruginosa strain AR_0095]
MSLASSLESLRKIDVNDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLRDLQTQLEQQAAQEESLKQQFATKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVTQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPASPDNTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=235152 AM474_RS17670 WP_003116135.1 3640563..3641186(+) (pilO) [Pseudomonas aeruginosa strain AR_0095]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGACGTAAACGACCTCGATCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTCCGTGACT
TGCAAACACAGCTCGAACAGCAGGCTGCGCAAGAAGAATCGCTCAAGCAGCAATTTGCTACCAAAGCGTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAGGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGTCTGGGCAGCGGCCTGGAGTTCGAGGAGATCAAGCTGCTCC
CTGAAGTCACTCAGCAGTTCTATATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTTACCCTGCATGACTTCGAGATCAAGCCGGCTTCGCCCGATAACACCTC
GAAGCTGCGCATGAGCATCTTGGCCAAGACCTACCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0H2ZIF6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

45.596

93.237

0.425
  pilO Acinetobacter baumannii D1279779

45.789

91.787

0.42
  comO/pilO Acinetobacter baylyi ADP1

43.617

90.821

0.396

Multiple sequence alignment