NTDB

Overview

Name	ciaR	Type	Regulator
Locus tag	AM474_RS01130	Genome accession	NZ_CP027538
Coordinates	246255..246926 (-)	Length	223 a.a.
NCBI ID	WP_003090494.1	Uniprot ID	A0A0H2ZC88
Organism	Pseudomonas aeruginosa strain AR_0095
Function	repress competence development (predicted from homology) Competence regulation

Genomic Context

Location: 241255..251926

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AM474_RS33055	-	242592..243611 (+)	1020	Protein_218	chemotaxis protein	-
AM474_RS33060	-	243981..244517 (+)	537	Protein_219	methyl-accepting chemotaxis protein	-
AM474_RS01120 (AM474_01120)	-	244522..244830 (-)	309	WP_003097699.1	hypothetical protein	-
AM474_RS01125 (AM474_01125)	carS	244921..246258 (-)	1338	WP_003090493.1	two-component system sensor histidine kinase CarS	-
AM474_RS01130 (AM474_01130)	ciaR	246255..246926 (-)	672	WP_003090494.1	two-component system response regulator CarR	Regulator
AM474_RS01135 (AM474_01135)	-	246926..247240 (-)	315	WP_003131079.1	PepSY domain-containing protein	-
AM474_RS01140 (AM474_01140)	-	247240..247548 (-)	309	WP_003090497.1	PepSY domain-containing protein	-
AM474_RS01145 (AM474_01145)	-	247763..248845 (+)	1083	WP_003143813.1	hypothetical protein	-
AM474_RS01150 (AM474_01150)	-	248872..249765 (+)	894	WP_003131077.1	hypothetical protein	-
AM474_RS01155 (AM474_01155)	-	249749..250015 (+)	267	WP_003090500.1	DUF5329 domain-containing protein	-
AM474_RS01160 (AM474_01160)	-	250016..251209 (-)	1194	WP_034027070.1	NnrS family protein	-
AM474_RS01165 (AM474_01165)	ppyR	251196..251453 (-)	258	WP_003090502.1	psl/pyoverdine operon transcriptional regulator PpyR	-

Sequence

Protein

Download Length: 223 a.a. Molecular weight: 24729.20 Da Isoelectric Point: 5.8445

                                >NTDB_id=235095 AM474_RS01130 WP_003090494.1 246255..246926(-) (ciaR) [Pseudomonas aeruginosa strain AR_0095]

MRLLLVEDHVPLADELMASLTRQGYAVDWLADGRDAAVQGASEPYDLIILDLGLPGRPGLEILQEWRGLGLATPVLILTA

RGSWAERIDGLKAGADDYLTKPFHPEELALRIQALLRRAHGLANQSQLEAAGLRLDEQRQCVCLNGADVDLTAAEFRLLR

YFMLHPGQVLSKGHLAEHLYDGETERDSNVIEVHVNHLRRKLGREVIETRRGQGYRYAGVAAG

Nucleotide

Download Length: 672 bp

                                >NTDB_id=235095 AM474_RS01130 WP_003090494.1 246255..246926(-) (ciaR) [Pseudomonas aeruginosa strain AR_0095]

ATGCGGTTGCTGCTGGTTGAGGATCACGTTCCCCTGGCCGACGAACTGATGGCCAGCCTGACCCGCCAGGGCTACGCGGT

GGACTGGCTGGCCGACGGTCGCGACGCGGCGGTGCAGGGCGCCAGCGAGCCCTACGACCTGATCATCCTCGACCTCGGCC

TGCCCGGGCGCCCCGGCCTGGAGATACTCCAGGAGTGGCGTGGGCTGGGACTCGCGACTCCGGTGCTGATCCTCACCGCG

CGCGGTTCCTGGGCCGAACGCATCGATGGCCTCAAGGCCGGTGCCGACGACTACCTGACCAAGCCGTTCCATCCGGAAGA

GTTGGCCTTGCGCATCCAGGCCCTGTTGCGCCGCGCCCATGGGCTGGCCAACCAGAGCCAGCTGGAGGCCGCTGGGCTGC

GCCTGGACGAGCAGCGCCAGTGCGTGTGCCTGAACGGCGCCGACGTCGACCTGACCGCCGCCGAATTCCGCCTGCTGCGC

TATTTCATGCTGCACCCCGGCCAGGTCCTGTCCAAGGGGCACCTGGCCGAGCATCTGTACGACGGCGAGACCGAACGCGA

TTCCAATGTCATCGAGGTCCACGTCAACCACTTGCGGCGCAAGCTCGGCCGCGAGGTGATCGAGACCCGCCGTGGCCAGG

GCTACCGCTACGCCGGGGTGGCCGCCGGATGA

Domains

Predicted by InterProScan.

Response regulator receiver domain (Response_reg)

(4-112)

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(147-216)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure
AlphaFold DB	A0A0H2ZC88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
ciaR	Streptococcus mutans UA159	37.727	98.655	0.372
ciaR	Streptococcus pneumoniae Rx1	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae D39	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae R6	37.273	98.655	0.368
ciaR	Streptococcus pneumoniae TIGR4	37.273	98.655	0.368
micA	Streptococcus pneumoniae Cp1015	35.683	100	0.363
vicR	Streptococcus mutans UA159	35.526	100	0.363

Detailed information NTG0235095