Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYF   Type   Machinery gene
Locus tag   M6_RS00915 Genome accession   NC_006086
Coordinates   145782..146216 (+) Length   144 a.a.
NCBI ID   WP_002986542.1    Uniprot ID   -
Organism   Streptococcus pyogenes MGAS10394     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 140782..151216
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M6_RS00885 (M6_Spy0133) - 142451..142816 (+) 366 WP_002986560.1 DUF1033 family protein -
  M6_RS00890 (M6_Spy0134) comYA 142909..143847 (+) 939 WP_002986557.1 competence type IV pilus ATPase ComGA Machinery gene
  M6_RS00895 (M6_Spy0135) comYB 143783..144817 (+) 1035 WP_011184088.1 competence type IV pilus assembly protein ComGB Machinery gene
  M6_RS00900 (M6_Spy0136) comYC 144819..145145 (+) 327 WP_002986552.1 competence type IV pilus major pilin ComGC Machinery gene
  M6_RS00905 (M6_Spy0137) comGD 145120..145548 (+) 429 WP_011184089.1 competence type IV pilus minor pilin ComGD -
  M6_RS00910 (M6_Spy0138) comGE 145505..145789 (+) 285 WP_027968839.1 competence type IV pilus minor pilin ComGE -
  M6_RS00915 (M6_Spy0139) comYF 145782..146216 (+) 435 WP_002986542.1 competence type IV pilus minor pilin ComGF Machinery gene
  M6_RS00920 (M6_Spy0140) comGG 146200..146526 (+) 327 WP_002986539.1 competence type IV pilus minor pilin ComGG -
  M6_RS00925 (M6_Spy0141) comYH 146624..147577 (+) 954 WP_021340467.1 class I SAM-dependent methyltransferase Machinery gene
  M6_RS00930 (M6_Spy0142) - 147636..148832 (+) 1197 WP_002986533.1 acetate kinase -
  M6_RS00935 (M6_Spy0143) - 149019..149327 (+) 309 WP_011017251.1 hypothetical protein -
  M6_RS00940 (M6_Spy0144) proC 149410..150180 (-) 771 WP_011184092.1 pyrroline-5-carboxylate reductase -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16573.20 Da        Isoelectric Point: 10.3584

>NTDB_id=23429 M6_RS00915 WP_002986542.1 145782..146216(+) (comYF) [Streptococcus pyogenes MGAS10394]
MSKQLSNIKAFTLLEALIALLVISGSLLVYQGLTQTLLKRSHYLARHDQDNWLLFSHQLREELSGARFYKVADNKLYVEK
GKKVLAFGQFKSHDFRKSASNGKGYQPMLFGISRSHIHIGQSQICITLKWKSGLERTFYYAFQD

Nucleotide


Download         Length: 435 bp        

>NTDB_id=23429 M6_RS00915 WP_002986542.1 145782..146216(+) (comYF) [Streptococcus pyogenes MGAS10394]
TTGAGTAAACAATTAAGTAACATAAAAGCTTTTACCCTTCTAGAGGCATTAATAGCCTTACTCGTGATATCAGGGTCTTT
ATTGGTTTATCAAGGTTTGACCCAAACCCTCCTTAAACGTAGCCATTATCTAGCCCGTCATGATCAAGACAATTGGCTCT
TATTTTCTCATCAATTGCGAGAGGAGTTAAGTGGAGCAAGATTTTACAAAGTAGCTGATAATAAACTATACGTTGAAAAG
GGAAAGAAAGTACTAGCTTTTGGCCAATTTAAAAGTCATGATTTCCGAAAATCAGCTAGTAATGGAAAAGGGTATCAACC
CATGTTATTTGGAATATCACGTAGTCATATTCACATAGGGCAGTCACAGATTTGCATTACTTTAAAGTGGAAAAGTGGGT
TAGAAAGGACTTTTTATTATGCCTTTCAAGACTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(41-135)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYF Streptococcus mutans UA140

51.079

96.528

0.493
  comYF Streptococcus mutans UA159

50.36

96.528

0.486
  comGF Lactococcus lactis subsp. cremoris KW2

45.985

95.139

0.437
  comGF/cglF Streptococcus mitis NCTC 12261

44.03

93.056

0.41
  comGF/cglF Streptococcus pneumoniae Rx1

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae D39

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae R6

43.609

92.361

0.403
  comGF/cglF Streptococcus pneumoniae TIGR4

43.609

92.361

0.403
  comGF/cglF Streptococcus mitis SK321

42.857

92.361

0.396

Multiple sequence alignment