Detailed information NTG0232199

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: CCZ28_RS11425
• Genome accession: NZ_CP021645
• Coordinates: 2567928..2569622 (-)
• Length: 564 a.a.
• NCBI ID: WP_140221328.1
• Uniprot ID: -
• Organism: Pseudomonas oryzihabitans strain CS51
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
IS/Tn	2570538..2570915	2567928..2569622	flank	916

Gene organization within MGE regions

Location: 2567928..2570915

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CCZ28_RS11425 (CCZ28_11400)	pilB	2567928..2569622 (-)	1695	WP_140221328.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CCZ28_RS11430 (CCZ28_11405)	-	2569835..2570371 (+)	537	WP_140218115.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-

Sequence

Protein

• Length: 564 a.a.
• Molecular weight: 62338.68 Da
• Isoelectric Point: 5.5870

>NTDB_id=232199 CCZ28_RS11425 WP_140221328.1 2567928..2569622(-) (pilB) [Pseudomonas oryzihabitans strain CS51]
MNEIVTLSGLARQLVDAQLLAEPAAQQAVVKAKQAKIPLVTYLVQQRLVQSRPLAELAAQEFGVPFLDLAAIDKDQLPKD
IVNEKLMQQHHFLPLSKRGNKLFIALSDPTNQQAINDVSFGSKLAVEVVLVEDDKLHQAIERYLDTGTGGLDLGDIDLDL
EADGGERPQEAVAGIDADDAPVVKFVNKMLLDAIKKGSSDLHFEPYEKMYRVRFRTDGILHEAARPPVQLVSRISARLKV
MSNMDISERRKPQDGRVKLKVSKTKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEDVQKELYLQALKQPQGMI
LVTGPTGSGKTVSLYTGLNILNTEDINISTAEDPVEINLEGINQVNVNPRQGLDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNLATSVNLIIAQRLARKLCTKCKRVHEVPHEALLAEGFKEEE
IGSFTLYQPIGCEDCNGGYRGRVGIYEVVKITPALQQMIMEEGNSIEIAARMRTEGFNDLRRSGLVKAMQGITSLEEINR
VTKD

Nucleotide

• Length: 1695 bp

>NTDB_id=232199 CCZ28_RS11425 WP_140221328.1 2567928..2569622(-) (pilB) [Pseudomonas oryzihabitans strain CS51]
ATGAACGAGATAGTGACGCTGTCGGGGTTGGCGCGGCAGCTGGTGGATGCGCAGTTGCTGGCAGAGCCGGCCGCGCAGCA
GGCGGTGGTCAAGGCCAAGCAGGCCAAGATACCGCTGGTGACCTATCTCGTTCAGCAGCGATTGGTACAAAGCCGGCCTC
TGGCAGAGTTAGCCGCTCAGGAATTTGGTGTGCCTTTTCTCGATCTGGCCGCCATTGATAAAGATCAACTGCCCAAGGAC
ATCGTCAACGAGAAGCTGATGCAGCAGCATCACTTCCTGCCTCTGAGCAAGAGAGGCAACAAGCTTTTCATCGCACTCTC
TGACCCTACCAACCAGCAGGCCATCAATGATGTGTCTTTTGGCAGCAAGCTGGCGGTCGAGGTCGTGCTGGTTGAGGATG
ACAAGCTGCATCAGGCCATCGAGCGATATCTGGATACCGGCACGGGGGGCCTGGACCTAGGCGATATTGATCTTGATCTG
GAAGCTGATGGCGGTGAGCGGCCTCAGGAGGCTGTAGCCGGTATCGATGCCGACGATGCGCCAGTAGTGAAGTTCGTCAA
CAAGATGCTGCTCGACGCCATCAAGAAGGGCTCGTCCGACCTCCACTTCGAGCCCTATGAAAAGATGTACCGGGTGCGTT
TTCGTACCGATGGCATCTTGCACGAGGCGGCGCGGCCACCGGTGCAGCTCGTTTCGCGCATCTCGGCGCGGCTCAAGGTG
ATGTCCAACATGGATATCTCCGAGCGCCGCAAGCCCCAGGACGGTCGGGTCAAGCTCAAGGTGTCCAAGACCAAGTCCAT
CGACTTCCGGGTCAATACCTTGCCCACGCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACTCTTCCAGTGCTCAGA
TGGGGATCGATGCCCTGGGCTACGAGGATGTCCAGAAGGAGCTCTACTTACAGGCGCTCAAGCAGCCGCAGGGGATGATT
CTGGTAACCGGACCGACGGGTTCGGGCAAGACGGTGTCGCTCTACACCGGCCTCAATATCCTCAATACCGAAGACATCAA
CATTTCCACTGCGGAAGATCCGGTGGAAATCAATCTGGAAGGCATCAACCAGGTCAACGTCAATCCACGGCAGGGGCTCG
ACTTCTCCCAGGCGTTGCGCGCCTTCCTGCGCCAGGACCCCGATGTGATCATGGTGGGGGAGATTCGTGATCTTGAGACG
GCGGAGATCGCCATCAAGGCGGCCCAGACCGGTCACATGGTGATGTCGACGCTGCACACCAACAGTGCAGCGGAAACCCT
GACACGCTTGCGTAATATGGGTGTACCAGCCTTCAACTTGGCGACCTCGGTCAACCTGATCATCGCCCAACGGCTGGCTC
GCAAGCTGTGCACCAAGTGCAAGCGCGTCCATGAGGTTCCTCATGAGGCTTTGCTGGCAGAGGGGTTTAAGGAAGAAGAG
ATTGGCAGCTTCACGCTGTATCAGCCCATTGGTTGCGAGGACTGCAACGGGGGTTATCGCGGTCGAGTAGGGATCTATGA
GGTGGTGAAGATCACTCCCGCGTTGCAGCAGATGATCATGGAGGAGGGCAACTCCATCGAGATCGCGGCGCGGATGCGGA
CGGAGGGGTTCAACGATCTGCGGCGCTCCGGCCTGGTGAAGGCGATGCAGGGGATTACCAGTCTGGAAGAGATCAATCGG
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (188-455)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	60.071	99.468	0.598
pilB	Acinetobacter baumannii D1279779	59.609	99.645	0.594
pilB	Legionella pneumophila strain ERS1305867	55.124	100	0.553
pilB	Vibrio cholerae strain A1552	50.709	100	0.507
pilB	Vibrio campbellii strain DS40M4	50.178	99.645	0.5
pilB	Vibrio parahaemolyticus RIMD 2210633	50.089	99.113	0.496
pilF	Neisseria gonorrhoeae MS11	49.286	99.291	0.489
pilF	Thermus thermophilus HB27	37.363	96.809	0.362