Detailed information NTG0023216

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSPPH_RS11750
• Genome accession: NC_005773
• Coordinates: 2693533..2694702 (+)
• Length: 389 a.a.
• NCBI ID: WP_011168499.1
• Uniprot ID: Q48JA6
• Organism: Pseudomonas savastanoi pv. phaseolicola 1448A
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2688533..2699702

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSPPH_RS11735 (PSPPH_2316)	-	2688970..2691297 (+)	2328	WP_019741978.1	ATP-dependent DNA helicase	-
PSPPH_RS11740 (PSPPH_2317)	pgm	2691413..2693059 (+)	1647	WP_011168498.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSPPH_RS11745 (PSPPH_2318)	-	2693134..2693409 (-)	276	WP_002553334.1	peptidylprolyl isomerase	-
PSPPH_RS11750 (PSPPH_2319)	pilU	2693533..2694702 (+)	1170	WP_011168499.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSPPH_RS11755 (PSPPH_2320)	-	2694751..2696328 (-)	1578	WP_011168500.1	ABC transporter ATP-binding protein	-
PSPPH_RS11760 (PSPPH_2321)	-	2696330..2697352 (-)	1023	WP_011168501.1	ABC transporter permease	-
PSPPH_RS11765 (PSPPH_2322)	-	2697352..2698416 (-)	1065	WP_011168502.1	microcin C ABC transporter permease YejB	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42552.70 Da
• Isoelectric Point: 6.0634

>NTDB_id=23216 PSPPH_RS11750 WP_011168499.1 2693533..2694702(+) (pilU) [Pseudomonas savastanoi pv. phaseolicola 1448A]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLGSETLKPGEVAVIAQGLMDEEQKLEFLRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVRTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMDKSGSLGMQTFDTAL
FNLAVEGAISEEEALKNADSQNNVRLRLKLHSEGGVASLTTPPPAPTGSSTASTAEWGLVDDDAPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=23216 PSPPH_RS11750 WP_011168499.1 2693533..2694702(+) (pilU) [Pseudomonas savastanoi pv. phaseolicola 1448A]
ATGGATTTTCCGGCGCTGTTGAAGATTCTGGCCAGTCAGGACGGATCGGACCTGTATCTTTCCACCGGAGCGCCTCCGTG
CGCCAAGTTCAACGGCGTGCTCAAGCCGCTGGGCAGTGAAACCCTCAAGCCGGGCGAGGTGGCGGTGATTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAATTCCTGCGCGAACTGGAGATGAACCTTGCTGTTTCGCTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGTAACGAGGTGTCCATCGTCGCGCGGAATATCAAGCTGGATATTCCGCGTTTCGA
GGACCTGTTCCTGCCGCCCGTACTGCTCGATGTGATCATGGAAAAGCACGGGCTGGTGTTGTTCGTCGGTGCCACCGGTT
CGGGCAAATCGACCTCGCTGGCGGCGCTGATCGATTACCGCAATCGCAACGCCAGCGGCCATATCATCACCATTGAAGAC
CCGGTCGAATTTATCCATCGGCACAAGAAGTCCATCGTCAACCAGCGCGAAGTGGGGGTTGATACCCGCAGTTTCCGCGC
GGCGCTGAAGAACACGTTGCGTCAGGCTCCGGATGTGATTCTGATCGGCGAAATTCGTGATCGCGAGACCATGGAGCATG
CGCTGGCGTTTGCCGACACCGGCCACCTGGCCATCTCGACCCTGCATGCCAACAACGCCAATCAGGCGCTGGACCGGATC
ATCAACTTCTTCCCGGAAGAGCGTCGTGCGCAGTTGCTGCATGACCTGGGCAATAACCTCAAGGCGTTCGTGTCCCAGCG
GCTGGTTAGAACCCCGGACGGCAAACGTCGCGCAGCGGTGGAGGTGATGATGGGCACGCCGACCATTCGCGATCTGATTC
AGCGCAATGAACTGACCGAACTCAAGGGCATCATGGATAAATCCGGCAGCCTCGGTATGCAGACCTTCGACACGGCGTTG
TTCAATCTGGCCGTCGAGGGGGCGATCAGCGAGGAGGAGGCGCTGAAGAATGCCGACTCGCAGAACAACGTTCGTCTGCG
CCTTAAACTGCACAGTGAAGGCGGAGTCGCCAGCCTGACGACGCCGCCCCCGGCCCCGACCGGCAGCAGCACGGCAAGTA
CCGCCGAGTGGGGGCTGGTCGATGACGACGCGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-270)

3D structure

• AlphaFold DB: Q48JA6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	58.011	93.059	0.54
pilU	Acinetobacter baylyi ADP1	51.541	91.774	0.473
pilU	Vibrio cholerae strain A1552	51.714	89.974	0.465
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.687	98.458	0.391
pilT	Pseudomonas aeruginosa PAK	41.108	88.175	0.362