Detailed information NTG0023055

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: CV_RS00825
• Genome accession: NC_005085
• Coordinates: 189188..190324 (+)
• Length: 378 a.a.
• NCBI ID: WP_011133735.1
• Uniprot ID: -
• Organism: Chromobacterium violaceum ATCC 12472
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 184188..195324

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CV_RS00795 (CV0174)	dksA	184727..185137 (-)	411	WP_011133729.1	RNA polymerase-binding protein DksA	-
CV_RS00800 (CV0175)	-	185421..185726 (-)	306	WP_011133730.1	c-type cytochrome	-
CV_RS00805 (CV0176)	-	185806..186387 (-)	582	WP_011133731.1	YggT family protein	-
CV_RS00810 (CV0177)	proC	186397..187200 (-)	804	WP_011133732.1	pyrroline-5-carboxylate reductase	-
CV_RS00815 (CV0178)	-	187255..187950 (-)	696	WP_011133733.1	YggS family pyridoxal phosphate-dependent enzyme	-
CV_RS00820 (CV0179)	pilT	188088..189125 (+)	1038	WP_011133734.1	type IV pilus twitching motility protein PilT	Machinery gene
CV_RS00825 (CV0180)	pilU	189188..190324 (+)	1137	WP_011133735.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
CV_RS00830 (CV0181)	-	190350..191963 (+)	1614	WP_052262685.1	response regulator	-
CV_RS21915 (CV0182)	-	191998..193611 (+)	1614	WP_011133737.1	response regulator	-
CV_RS00840 (CV0183)	-	193611..194135 (+)	525	WP_011133738.1	2'-5' RNA ligase family protein	-
CV_RS00845 (CV0184)	-	194119..194904 (-)	786	WP_011133739.1	TIGR00266 family protein	-

Sequence

Protein

• Length: 378 a.a.
• Molecular weight: 42305.49 Da
• Isoelectric Point: 6.0195

>NTDB_id=23055 CV_RS00825 WP_011133735.1 189188..190324(+) (pilU) [Chromobacterium violaceum ATCC 12472]
MEKDQASKFIHDLLKHATGKNASDIFIAAEFPPAMKIDGKITPVAPQALTALQSKELVRAVMNDRQTEEFEAKKEANFAI
NPPGVGRFRVSAFVQQGMVGMVLRKINTEIPTFDQLNLPEVLKDIALIKRGLVIFVGGTGSGKSTSLAALVDWRNSNNQD
HIITIEDPIEYVHTHKKSIVTQREIGVDTENWEVALKNTLRQAPDVILMGEIRDRETMAYGLQFAETGHLCLATLHANNA
NQALDRILNFFPEERHQQVLMDLSLNMRAIISQRLVPHKSGRGRVAAVEILLNSPLVSDMVFKGEIAGLKEVMGRSREAG
MQTFDQSLFELYEAELITYEDALRNADSINDLRLRIKLYSESARNRDPLEGIDHLDIV

Nucleotide

• Length: 1137 bp

>NTDB_id=23055 CV_RS00825 WP_011133735.1 189188..190324(+) (pilU) [Chromobacterium violaceum ATCC 12472]
ATGGAAAAAGACCAGGCCTCCAAATTCATCCACGACCTGCTCAAGCATGCGACGGGAAAGAACGCCTCCGACATCTTCAT
CGCCGCCGAATTTCCGCCGGCGATGAAGATAGACGGCAAGATCACGCCGGTGGCGCCGCAGGCGCTGACCGCCCTGCAGA
GCAAGGAACTGGTGCGCGCGGTGATGAACGACCGCCAGACCGAGGAGTTCGAGGCGAAGAAGGAAGCCAACTTCGCCATC
AACCCGCCCGGCGTCGGCCGTTTCCGCGTCAGCGCCTTCGTCCAGCAGGGCATGGTGGGCATGGTGCTGCGCAAGATCAA
CACCGAGATCCCCACCTTCGACCAGCTCAACCTGCCCGAGGTGCTGAAGGACATCGCGCTGATCAAGCGCGGCCTGGTGA
TCTTCGTCGGCGGCACCGGCTCCGGCAAGTCCACCTCGCTGGCGGCGCTGGTGGACTGGCGCAACAGCAACAACCAGGAC
CACATCATCACGATCGAAGACCCGATCGAGTACGTGCACACCCACAAGAAGTCCATCGTCACCCAGCGCGAGATCGGCGT
GGACACCGAGAACTGGGAGGTCGCGCTGAAGAACACGCTGCGCCAGGCGCCGGACGTGATCCTGATGGGGGAGATCCGCG
ACCGCGAAACCATGGCCTACGGCCTGCAGTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACGCCAACAACGCC
AACCAGGCGCTGGACCGCATCCTCAACTTCTTCCCCGAAGAGCGCCACCAGCAAGTGCTGATGGACCTGTCGCTGAACAT
GCGCGCCATCATTTCGCAGCGGCTGGTGCCGCACAAGTCCGGCCGCGGCCGGGTGGCGGCGGTGGAGATCCTGCTCAACA
GCCCGCTGGTGTCGGACATGGTGTTCAAGGGCGAGATCGCCGGGCTGAAGGAGGTGATGGGACGCTCGCGCGAGGCCGGC
ATGCAGACTTTCGACCAATCGCTGTTCGAACTGTACGAGGCCGAGCTGATCACTTACGAGGATGCGCTGCGCAACGCCGA
CTCCATCAACGACCTGCGCCTGAGAATCAAGCTGTACAGCGAGAGCGCCCGCAACCGGGACCCGCTGGAAGGCATAGACC
ACCTGGATATCGTCTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (14-277)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.857	92.593	0.563
pilU	Acinetobacter baylyi ADP1	54.918	96.825	0.532
pilU	Vibrio cholerae strain A1552	52.991	92.857	0.492
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	44.51	89.153	0.397
pilT	Pseudomonas aeruginosa PAK	40.653	89.153	0.362