Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   CBP33_RS18425 Genome accession   NZ_CP021359
Coordinates   3940151..3940726 (-) Length   191 a.a.
NCBI ID   WP_086913796.1    Uniprot ID   -
Organism   Acidovorax carolinensis strain NA2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3935151..3945726
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBP33_RS18395 (CBP33_18390) - 3935542..3935997 (+) 456 WP_086913790.1 hypothetical protein -
  CBP33_RS18400 (CBP33_18395) - 3936095..3936448 (+) 354 WP_086913791.1 phage holin family protein -
  CBP33_RS18405 (CBP33_18400) - 3936416..3937882 (-) 1467 WP_236749195.1 M48 family metalloprotease -
  CBP33_RS18410 (CBP33_18405) moaC 3938071..3938568 (+) 498 WP_086913793.1 cyclic pyranopterin monophosphate synthase MoaC -
  CBP33_RS18415 (CBP33_18410) - 3938649..3939437 (-) 789 WP_157896616.1 pilus assembly protein -
  CBP33_RS18420 (CBP33_18415) - 3939459..3940025 (-) 567 WP_086913795.1 pilin -
  CBP33_RS18425 (CBP33_18420) pilE 3940151..3940726 (-) 576 WP_086913796.1 pilin Machinery gene
  CBP33_RS18430 (CBP33_18425) - 3940947..3941738 (-) 792 WP_086913797.1 3',5'-cyclic-nucleotide phosphodiesterase -
  CBP33_RS18435 (CBP33_18430) - 3941839..3942498 (-) 660 WP_086913798.1 FHA domain-containing protein -
  CBP33_RS18440 (CBP33_18435) - 3942542..3943333 (-) 792 WP_086913799.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  CBP33_RS18445 (CBP33_18440) - 3943746..3944432 (-) 687 WP_086913800.1 TerC family protein -
  CBP33_RS18450 (CBP33_18445) sucD 3944569..3945465 (-) 897 WP_086913801.1 succinate--CoA ligase subunit alpha -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 19322.22 Da        Isoelectric Point: 8.4764

>NTDB_id=230168 CBP33_RS18425 WP_086913796.1 3940151..3940726(-) (pilE) [Acidovorax carolinensis strain NA2]
MKRTLQKGFTLIELMIVVAIIGILAAVALPAYQDYTIRAKVSEAVIAASSAKSVLSEAFQTGGVTALNAAGTGINLTAAA
EKASKYVVNYCVDVAPADTCAALAADGTGPWQINVAIAATSANGIPTGLNANVLRFLPRVQNATPTAALSGAVDWACASS
TNTTATARFTGIAFTGVGTALPAKYAPAECR

Nucleotide


Download         Length: 576 bp        

>NTDB_id=230168 CBP33_RS18425 WP_086913796.1 3940151..3940726(-) (pilE) [Acidovorax carolinensis strain NA2]
ATGAAGCGTACTCTGCAAAAGGGTTTTACCCTGATCGAACTGATGATCGTTGTGGCGATCATCGGTATTTTGGCTGCCGT
GGCACTGCCTGCGTACCAGGACTACACCATCCGCGCCAAAGTTTCTGAAGCTGTTATCGCAGCATCATCGGCAAAATCTG
TTCTGAGTGAGGCCTTCCAAACGGGCGGCGTTACTGCGTTGAATGCGGCTGGCACCGGTATCAATCTAACTGCCGCCGCT
GAAAAAGCCTCCAAATACGTGGTCAATTATTGTGTTGACGTTGCCCCTGCAGATACCTGCGCCGCTCTTGCCGCCGATGG
CACAGGCCCATGGCAGATCAACGTTGCGATCGCTGCCACTTCTGCGAACGGCATTCCTACTGGTCTGAATGCGAATGTCC
TTCGCTTCCTGCCGCGAGTACAAAACGCGACCCCAACCGCCGCGCTTTCTGGAGCAGTTGACTGGGCTTGTGCTAGCTCA
ACAAACACCACCGCTACGGCCCGTTTCACGGGTATTGCTTTCACAGGTGTAGGTACCGCACTGCCTGCCAAGTATGCTCC
GGCCGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(38-191)

Prokaryotic N-terminal methylation motif (N_methyl)

(6-29)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

55.721

100

0.586
  comP Acinetobacter baylyi ADP1

40.107

97.906

0.393
  pilA2 Legionella pneumophila str. Paris

38.86

100

0.393
  pilA Ralstonia pseudosolanacearum GMI1000

37.949

100

0.387
  pilA2 Legionella pneumophila strain ERS1305867

38.421

99.476

0.382

Multiple sequence alignment