Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA923_RS04675 Genome accession   NZ_CP021267
Coordinates   1011809..1012873 (+) Length   354 a.a.
NCBI ID   WP_011215094.1    Uniprot ID   Q5WXY1
Organism   Legionella pneumophila subsp. pneumophila strain Burlington     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1006809..1017873
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA923_RS04665 - 1007849..1010233 (-) 2385 WP_027220940.1 penicillin-binding protein 1A -
  CA923_RS04670 ravI 1010385..1011392 (-) 1008 WP_011946020.1 Dot/Icm T4SS effector RavI -
  CA923_RS04675 pilM 1011809..1012873 (+) 1065 WP_011215094.1 type IV pilus assembly protein PilM Machinery gene
  CA923_RS04680 pilN 1012877..1013425 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  CA923_RS04685 pilO 1013439..1014038 (+) 600 WP_013101267.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA923_RS04690 pilP 1014035..1014619 (+) 585 WP_013101268.1 pilus assembly protein PilP Machinery gene
  CA923_RS04695 pilQ 1014623..1016719 (+) 2097 WP_011946023.1 type IV pilus secretin PilQ Machinery gene
  CA923_RS04700 aroK 1017166..1017693 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38760.78 Da        Isoelectric Point: 5.0367

>NTDB_id=229412 CA923_RS04675 WP_011215094.1 1011809..1012873(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Burlington]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEDICVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDLEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKSSSLLDVLIVASRAENVNTRVEAAHR
AGLEPKVVDVESYAVERSVQQIAQELPASGQDKIVAIVDIGANYTHLFVLHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSHMSFGKKVNLDVINSDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229412 CA923_RS04675 WP_011215094.1 1011809..1012873(+) (pilM) [Legionella pneumophila subsp. pneumophila strain Burlington]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATCAGTTCTTCCGCAGTGAAAATTCTGGAAAT
ATCAGGTTCCGGAGAGGACATTTGTGTTGAAGGATATGGTCGTGAAGAAATACCTGCCTCTGCCCTAGAAGGAAATATGA
TCAAGGATGTCGATGTAGTAGCGAATTGCATCAAAAAATTGGTAGAAAGATTGAAGTTTACATCTAAAAATGTAGCATTG
GCTGTACCTGATTCGGCTGTTATCAGTAAAGTAGTGCAAATTAATGAAGGGTTAACTGACCTTGAAATGGAAGAGCTTGT
CGTTATTGAGGCGGATAAGTACATTCCTTACCCAATAGATGAAATAAACCTGGATTTTGAAGTGTTAGGCCATTCAGCCA
AGAGCTCATCACTGCTTGATGTTTTAATCGTCGCATCAAGGGCAGAAAACGTTAATACCCGTGTTGAAGCAGCCCATAGA
GCAGGGCTGGAACCCAAAGTTGTTGACGTTGAATCTTATGCAGTAGAGCGTTCAGTACAACAAATTGCGCAAGAATTACC
AGCAAGTGGGCAAGACAAAATTGTTGCAATAGTAGATATTGGAGCCAACTATACTCACTTATTTGTTTTGCATGGTATGC
GACTAATCTTTTCAAGAGAAGAAAAATTTGGAGGTATGCAACTTGTTGAAGCTATTGCAGAGCACTATCATATGAATTTG
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAACTACCGGAGGACTACGAATCTGCTGTATTAGAACCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGTACTTTGCAGTTTTTTTATTCAACCAGTCAACATGGATTTGTAGACCATATTTTATTGG
CGGGAGGAATGGCAAGACAACCAGGATTGGCAGCGCTTATTCAAGAACAGTTAGGAGTGACTACTACAGTGGCAAATCCA
CTATCCCATATGTCTTTTGGTAAAAAAGTGAATTTGGATGTGATTAACAGTGATGCACCTACACTGATGGTGGCTTGTGG
GTTGGCTTTGAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5WXY1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

100

100

1
  comM Acinetobacter nosocomialis M2

42.373

100

0.424
  pilM Acinetobacter baumannii D1279779

42.09

100

0.421
  comM Acinetobacter baylyi ADP1

40.678

100

0.407

Multiple sequence alignment