Detailed information NTG0229338

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: CA926_RS07455
• Genome accession: NZ_CP021265
• Coordinates: 1660223..1661947 (+)
• Length: 574 a.a.
• NCBI ID: WP_027227022.1
• Uniprot ID: A0A2S6EZP9
• Organism: Legionella pneumophila subsp. fraseri strain Los Angeles
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1655223..1666947

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CA926_RS07435	-	1655516..1658044 (+)	2529	WP_027227019.1	EAL domain-containing protein	-
CA926_RS07440	-	1658046..1658615 (-)	570	WP_027221706.1	hypoxanthine-guanine phosphoribosyltransferase	-
CA926_RS07445	-	1658658..1658984 (-)	327	WP_028378907.1	DUF1820 family protein	-
CA926_RS07450	-	1659167..1659910 (-)	744	WP_027227021.1	methyltransferase domain-containing protein	-
CA926_RS07455	pilB	1660223..1661947 (+)	1725	WP_027227022.1	type IV-A pilus assembly ATPase PilB	Machinery gene
CA926_RS07460	pilC	1661953..1663173 (+)	1221	WP_027221710.1	type II secretion system F family protein	Machinery gene
CA926_RS07465	pilD	1663219..1664082 (+)	864	WP_027227023.1	A24 family peptidase	Machinery gene
CA926_RS07470	-	1664096..1665301 (-)	1206	WP_028379061.1	cyclic nucleotide-binding domain-containing protein	-
CA926_RS07475	-	1665411..1666004 (-)	594	WP_027227025.1	hypothetical protein	-
CA926_RS07480	-	1666020..1666475 (-)	456	WP_027227026.1	hypothetical protein	-

Sequence

Protein

• Length: 574 a.a.
• Molecular weight: 62645.11 Da
• Isoelectric Point: 5.4362

>NTDB_id=229338 CA926_RS07455 WP_027227022.1 1660223..1661947(+) (pilB) [Legionella pneumophila subsp. fraseri strain Los Angeles]
MALATDEYRLQGIGQLLVLEKLLDKTKAIELHKLAAAEKISLVQYLVKNKILSAEQIALTAAQNFGVPMMDINCIEVDSI
PASLVNEKLIKRHAMVPLFSRGTNLYLATDDPSKQASLKEIQFHTGLNTHAIVVETDKLSSLIDNLLTAKESQGLSDYVE
DSGDLEGLEISTEDEDQDVDTATSATDDAPIVKFVNKILLDAIRQGASDIHFEPYEREYRIRYRQDGILHEVATPPASLA
SRITARIKVMSNLDISERRIPQDGGFKMKISKARAIDFRVSTCPTSAGEKVVMRVLDSGAAKLGIEALGFNPVQRSNFIK
AIQRPQGMILVTGPTGSGKTVTLYTALNILNTIEVNISTAEDPVEIKVPGINQVNINPKAGLTFSGALRSFLRQDPDIIM
VGEIRDLETAEIAVKAAQTGHLVLSTLHTNSAAETLNRLVNMGIPTFNIASSVTLIIAQRLARKLCNQCKAVRDDFTNQG
LIELGFKEADLVNLKLYKAVGCDQCTSGYRGRVGLFEVLPMTKELGQLIMSGGNSLDILKLAQSEGMLTIFQSGIEKVKE
GITTIEEVNRVTVD

Nucleotide

• Length: 1725 bp

>NTDB_id=229338 CA926_RS07455 WP_027227022.1 1660223..1661947(+) (pilB) [Legionella pneumophila subsp. fraseri strain Los Angeles]
ATGGCTCTAGCTACAGATGAATATAGACTACAGGGAATTGGCCAGCTTCTAGTACTGGAAAAGTTGCTAGATAAAACCAA
AGCAATTGAGTTGCATAAATTGGCCGCAGCAGAAAAGATATCTTTAGTGCAATATCTTGTAAAAAATAAAATACTATCTG
CTGAACAAATTGCACTGACGGCTGCTCAAAATTTTGGCGTACCAATGATGGATATTAATTGCATTGAGGTGGACTCTATT
CCTGCGAGTCTGGTTAATGAGAAATTGATTAAACGTCATGCCATGGTCCCCCTGTTTAGTCGCGGCACTAACTTGTACCT
TGCAACAGATGATCCCAGTAAGCAAGCTTCATTAAAGGAAATTCAATTTCATACCGGACTGAATACTCATGCTATAGTTG
TAGAAACAGATAAACTGAGTTCTTTGATTGATAACTTATTAACAGCAAAGGAAAGTCAAGGCTTATCGGACTATGTCGAA
GACTCTGGTGATCTGGAAGGTTTGGAAATAAGTACCGAAGATGAAGATCAAGATGTTGATACAGCAACATCTGCAACAGA
TGATGCACCGATTGTAAAGTTCGTTAATAAAATTTTATTGGATGCGATAAGGCAAGGCGCTTCTGATATACATTTTGAGC
CTTATGAACGAGAATACCGGATCAGGTACAGGCAAGATGGTATTTTACATGAGGTAGCTACCCCACCGGCAAGCCTGGCA
TCCCGTATTACAGCGCGTATCAAGGTAATGTCTAATTTGGATATATCAGAGAGGCGTATACCCCAGGATGGCGGCTTTAA
AATGAAAATATCCAAAGCACGAGCGATTGATTTCAGAGTGAGCACCTGTCCAACCTCAGCCGGGGAAAAAGTGGTGATGC
GAGTACTTGATTCTGGTGCTGCTAAATTAGGTATTGAAGCATTAGGATTTAATCCTGTTCAGAGGTCCAATTTCATTAAA
GCAATCCAGCGCCCACAGGGAATGATTTTAGTGACAGGCCCAACAGGTAGTGGAAAAACAGTTACCTTATATACGGCTTT
GAATATATTAAATACAATTGAAGTGAACATTTCGACTGCAGAGGATCCTGTTGAAATCAAGGTACCTGGCATCAATCAGG
TTAATATTAATCCTAAAGCCGGCCTAACCTTTTCCGGTGCATTGCGCTCGTTTCTAAGGCAAGACCCTGATATTATCATG
GTTGGTGAGATACGAGATCTTGAAACAGCTGAAATTGCTGTAAAAGCTGCGCAGACAGGGCATTTGGTTCTTTCTACACT
GCATACCAACAGTGCTGCTGAAACATTAAATCGTTTAGTGAACATGGGGATACCTACTTTTAACATTGCGAGTTCAGTTA
CATTAATTATTGCACAAAGGCTGGCAAGAAAATTATGTAATCAATGTAAGGCAGTGCGCGATGACTTTACCAATCAAGGT
TTGATTGAATTAGGCTTCAAGGAAGCTGATCTGGTTAATCTTAAATTATACAAAGCAGTAGGTTGTGATCAATGCACAAG
TGGCTATCGCGGGCGGGTTGGTCTGTTTGAGGTTTTACCAATGACGAAAGAGCTAGGCCAGTTGATTATGTCAGGTGGTA
ACTCCCTGGATATCTTAAAATTAGCCCAATCTGAAGGTATGCTGACTATCTTTCAATCGGGCATAGAAAAAGTCAAGGAA
GGGATCACAACCATAGAGGAGGTCAATCGGGTAACCGTTGATTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (197-464)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (46-151)

3D structure

• AlphaFold DB: A0A2S6EZP9

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Legionella pneumophila strain ERS1305867	96.341	100	0.963
pilB	Acinetobacter baumannii D1279779	51.146	98.78	0.505
pilB	Acinetobacter baylyi ADP1	49.315	100	0.502
pilB	Vibrio cholerae strain A1552	49.723	94.251	0.469
pilB	Vibrio campbellii strain DS40M4	47.397	97.038	0.46
pilF	Neisseria gonorrhoeae MS11	46.466	98.606	0.458
pilB	Vibrio parahaemolyticus RIMD 2210633	48.513	93.728	0.455
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.887	92.16	0.368
pilF	Thermus thermophilus HB27	38.806	93.38	0.362