Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CA928_RS05030 Genome accession   NZ_CP021264
Coordinates   1105777..1106841 (+) Length   354 a.a.
NCBI ID   WP_027227633.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. fraseri strain D-5387     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1100777..1111841
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA928_RS05020 - 1101816..1104200 (-) 2385 WP_027227631.1 penicillin-binding protein 1A -
  CA928_RS05025 ravI 1104351..1105358 (-) 1008 WP_027227632.1 Dot/Icm T4SS effector RavI -
  CA928_RS05030 pilM 1105777..1106841 (+) 1065 WP_027227633.1 type IV pilus assembly protein PilM Machinery gene
  CA928_RS05035 pilN 1106845..1107393 (+) 549 WP_027227634.1 PilN domain-containing protein Machinery gene
  CA928_RS05040 pilO 1107407..1108006 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA928_RS05045 pilP 1108003..1108587 (+) 585 WP_027227635.1 pilus assembly protein PilP Machinery gene
  CA928_RS05050 pilQ 1108591..1110687 (+) 2097 WP_027227636.1 type IV pilus secretin PilQ Machinery gene
  CA928_RS05055 aroK 1111133..1111660 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38826.80 Da        Isoelectric Point: 5.0331

>NTDB_id=229288 CA928_RS05030 WP_027227633.1 1105777..1106841(+) (pilM) [Legionella pneumophila subsp. fraseri strain D-5387]
MLKLLKPKHRSILGIDISSSAVKILEISGSGEQVCVEGYGREEIPASALEGNMIKDVDVVANCIKKLVERLKFTSKNVAL
AVPDSAVISKVVQINEGLTDQEMEELVVIEADKYIPYPIDEINLDFEVLGHSAKNSALLDVLIVASRAENVNSRVEAAHR
AGLEPKIVDVESYAVERSVQQIAQELPASGQDKIIAIVDIGTNYTHLFALHGMRLIFSREEKFGGMQLVEAIAEHYHMNL
EQAIALKNQGKLPEDYESAVLEPFKDMILLQIKRTLQFFYSTSQHGFVDHILLAGGMARQPGLAALIQEQLGVTTTVANP
LSYMSFGKKVNLDAINNDAPTLMVACGLALRHVE

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=229288 CA928_RS05030 WP_027227633.1 1105777..1106841(+) (pilM) [Legionella pneumophila subsp. fraseri strain D-5387]
GTGCTCAAATTGCTTAAACCTAAGCATCGTTCAATTCTTGGCATTGACATTAGTTCTTCAGCAGTGAAAATTTTGGAAAT
TTCAGGTTCCGGAGAGCAAGTTTGTGTTGAAGGATATGGTCGTGAAGAAATACCCGCCTCTGCTCTTGAAGGGAATATGA
TCAAGGACGTCGATGTAGTAGCGAATTGTATTAAAAAATTGGTAGAAAGGTTGAAGTTTACATCTAAAAATGTGGCATTG
GCTGTACCTGATTCGGCTGTTATTAGCAAAGTAGTGCAAATTAATGAAGGATTGACTGACCAGGAAATGGAAGAGCTTGT
TGTTATTGAAGCGGATAAGTATATTCCTTACCCAATAGATGAAATAAACCTTGATTTTGAAGTGTTAGGCCATTCTGCCA
AGAATTCAGCGCTGCTTGATGTTTTAATTGTGGCTTCAAGAGCAGAAAATGTTAATAGTCGTGTCGAGGCAGCTCATAGA
GCTGGCCTGGAGCCCAAAATCGTTGACGTTGAATCTTATGCGGTAGAACGTTCAGTGCAACAAATTGCGCAAGAATTGCC
AGCAAGTGGACAAGACAAAATTATAGCGATAGTAGATATTGGAACCAATTACACTCATTTATTTGCTTTGCATGGCATGC
GATTAATCTTTTCAAGAGAAGAAAAATTTGGTGGCATGCAGCTTGTTGAAGCTATTGCAGAACATTATCATATGAATTTA
GAGCAGGCTATTGCCCTAAAAAATCAGGGTAAATTACCAGAGGACTATGAATCTGCTGTATTAGAGCCTTTTAAGGACAT
GATTCTTTTGCAGATCAAACGCACTTTGCAGTTTTTTTATTCTACCAGTCAACATGGATTTGTTGACCATATTCTATTAG
CTGGAGGAATGGCAAGGCAACCAGGGTTGGCAGCGCTTATTCAAGAGCAATTAGGAGTGACTACTACAGTAGCAAATCCA
CTATCCTATATGTCTTTTGGTAAAAAAGTGAATTTGGATGCCATTAATAATGACGCGCCTACACTGATGGTGGCTTGTGG
GTTAGCTCTAAGGCATGTCGAGTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-352)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Legionella pneumophila strain ERS1305867

96.328

100

0.963
  comM Acinetobacter nosocomialis M2

42.655

100

0.427
  pilM Acinetobacter baumannii D1279779

42.373

100

0.424
  comM Acinetobacter baylyi ADP1

40.395

100

0.404

Multiple sequence alignment