Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CA928_RS05040 Genome accession   NZ_CP021264
Coordinates   1107407..1108006 (+) Length   199 a.a.
NCBI ID   WP_027221266.1    Uniprot ID   -
Organism   Legionella pneumophila subsp. fraseri strain D-5387     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1102407..1113006
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA928_RS05025 ravI 1104351..1105358 (-) 1008 WP_027227632.1 Dot/Icm T4SS effector RavI -
  CA928_RS05030 pilM 1105777..1106841 (+) 1065 WP_027227633.1 type IV pilus assembly protein PilM Machinery gene
  CA928_RS05035 pilN 1106845..1107393 (+) 549 WP_027227634.1 PilN domain-containing protein Machinery gene
  CA928_RS05040 pilO 1107407..1108006 (+) 600 WP_027221266.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA928_RS05045 pilP 1108003..1108587 (+) 585 WP_027227635.1 pilus assembly protein PilP Machinery gene
  CA928_RS05050 pilQ 1108591..1110687 (+) 2097 WP_027227636.1 type IV pilus secretin PilQ Machinery gene
  CA928_RS05055 aroK 1111133..1111660 (+) 528 WP_010946667.1 shikimate kinase AroK -
  CA928_RS05060 aroB 1111647..1112750 (+) 1104 WP_027227637.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 199 a.a.        Molecular weight: 22441.13 Da        Isoelectric Point: 5.3305

>NTDB_id=229290 CA928_RS05040 WP_027221266.1 1107407..1108006(+) (pilO) [Legionella pneumophila subsp. fraseri strain D-5387]
MDTVNLSELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLDTLEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSSGDGKTVSQDELVMGITAKIYRYRAQ

Nucleotide


Download         Length: 600 bp        

>NTDB_id=229290 CA928_RS05040 WP_027221266.1 1107407..1108006(+) (pilO) [Legionella pneumophila subsp. fraseri strain D-5387]
ATGGATACAGTCAATTTAAGTGAATTAACTCTGGAGAATGTCGGCCAGTGGCCAATGCCGGTTAAAATCGGAGTCATTTT
AGGCATCAGTATATTGATAATTGGATTGGGATATTGGCTTATTGTTAAGCCTAATTTTGAGCAATTGGATACACTGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAACAACATCAGGCAGCAAATCTACAAGCCTATCGTAATCAGTTA
CAGTTAATGAATGAACGCTTCGGAACCATGCTAAAACAACTACCGGCAAAAAATGAAATGCCTGGTCTTTTGGAAGAAAT
ATCTAAAACAGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAAGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTAGCAAGATTTTTAAGTCGTGTCGCTGAAATGAACAGA
ATCGTGACTCTGCATGAATTTAGTGTTGAGGGGGTATCCTCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGAT
GGGTATCACCGCTAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-197)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

97.487

100

0.975
  comO Acinetobacter baylyi ADP1

39.894

94.472

0.377
  pilO Acinetobacter baumannii D1279779

38.22

95.98

0.367

Multiple sequence alignment