Detailed information NTG0022798

Bioinformatically predicted

Overview

• Name: comEC/celB
• Type: Machinery gene
• Locus tag: SPYM3_RS05675
• Genome accession: NC_004070
• Coordinates: 1115184..1117427 (-)
• Length: 747 a.a.
• NCBI ID: WP_011054660.1
• Uniprot ID: A0A0H2UUX7
• Organism: Streptococcus pyogenes MGAS315
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1110184..1122427

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
SPYM3_RS05645 (SpyM3_1067)	-	1110823..1112043 (+)	1221	WP_011054655.1	DUF3114 domain-containing protein	-
SPYM3_RS05650 (SpyM3_1068)	-	1112157..1112747 (-)	591	WP_002983984.1	serine hydrolase	-
SPYM3_RS05655 (SpyM3_1069)	-	1112744..1112992 (-)	249	WP_011054656.1	hypothetical protein	-
SPYM3_RS05660 (SpyM3_1070)	-	1112977..1113204 (-)	228	WP_011054657.1	hypothetical protein	-
SPYM3_RS05665 (SpyM3_1071)	sodA	1113371..1113976 (-)	606	WP_011054658.1	superoxide dismutase SodA	-
SPYM3_RS05670 (SpyM3_1072)	holA	1114073..1115113 (-)	1041	WP_011054659.1	DNA polymerase III subunit delta	-
SPYM3_RS05675 (SpyM3_1073)	comEC/celB	1115184..1117427 (-)	2244	WP_011054660.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
SPYM3_RS05680 (SpyM3_1074)	comEA/celA/cilE	1117408..1118070 (-)	663	WP_002995626.1	ComEA family DNA-binding protein	Machinery gene
SPYM3_RS05685 (SpyM3_1075)	-	1118270..1119010 (-)	741	WP_011054661.1	lysophospholipid acyltransferase family protein	-
SPYM3_RS05690 (SpyM3_1076)	-	1119128..1119904 (+)	777	WP_002994360.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
SPYM3_RS05695 (SpyM3_1077)	-	1119894..1120172 (+)	279	WP_002989164.1	GIY-YIG nuclease family protein	-
SPYM3_RS05700 (SpyM3_1078)	-	1120196..1122196 (-)	2001	WP_011054663.1	potassium transporter Kup	-

Sequence

Protein

• Length: 747 a.a.
• Molecular weight: 85632.55 Da
• Isoelectric Point: 9.7404

>NTDB_id=22798 SPYM3_RS05675 WP_011054660.1 1115184..1117427(-) (comEC/celB) [Streptococcus pyogenes MGAS315]
MTFSWTKLVPLSKIQFAFLILVLFYQIHSPSWLTFLLSLSLICLLVKRLSKKEFLGVFAILSFCALFLLYQKQQLVQKLE
IQPVQITSVALVPDSIRINGDQLAVLGRHGKHSYQLFYRLKSQAEAQLFKKEHRWLVMHAKVTLEKAEEVRNFKGFNYQT
FLAYQGIYRIGKVEQIEQLEVISPESICDYLSSLRRRAIVHCQQHFPRRMSHYLTGLLFGYLDKSFGEMTDYYSQLGIIH
LFALSGMQVGFFLTCFRRVLLLLAVPLEWIKWIELPFACFYAALTGYSISVIRSLVQSQLRHLGIKGLDNLACTFLLVFL
WDAHFLMTVGGVLTFSYAFLLTVVTVEELSGAKRQLVQVLTISLGILPFLLFYFSSFNPMSMVLTALLSYLFDLFILPLL
CLVFCLSPLVTVSICNHLFILLEKVIQFLGNTFNSSLVFGSPTSWHLLILVISFAIFYDYRQVRQRVITCGLVIALTLLS
VKYPLINEVTFIDIGQGDSILVREWTGKNLLIDVGGRPFFSSKEHWRRGHHVANAQKTLIPYLKSRGIHTIDQLLVTHAD
TDHMGDIEVVAKAIRIKEILTSQGSLSHPSFVRRLRRLKYHVRVLAAGDQLPIMGSVLQVLYPWQLGDGKNNDSLVLYGR
LLNRTFLFTGDLEKEGENEIIKRYPQLRVDYLKAGHHGSNTSSSAAFLDHIQPKVAFISAGKNNRYQHPHRETLARLEDR
QITYYRTDTQGAIRLTGWTSWHLETVR

Nucleotide

• Length: 2244 bp

>NTDB_id=22798 SPYM3_RS05675 WP_011054660.1 1115184..1117427(-) (comEC/celB) [Streptococcus pyogenes MGAS315]
ATGACATTTTCTTGGACTAAGCTAGTGCCCCTGTCAAAGATTCAATTTGCTTTTTTAATCTTGGTTCTCTTTTATCAAAT
CCATAGCCCTAGCTGGTTAACGTTTCTCCTTAGCCTTAGTTTGATTTGCTTACTTGTCAAGCGTTTGTCTAAAAAAGAAT
TTCTCGGTGTCTTTGCCATCCTTAGTTTCTGTGCTTTGTTTTTGCTGTATCAGAAACAGCAGTTGGTTCAAAAACTGGAA
ATTCAGCCAGTACAAATAACCTCTGTAGCATTAGTTCCTGATAGTATTCGGATTAATGGTGATCAATTAGCTGTTCTTGG
ACGTCATGGGAAACATTCTTATCAGTTATTTTACCGTTTGAAAAGCCAAGCAGAAGCCCAATTGTTCAAGAAAGAGCACC
GTTGGTTAGTGATGCACGCAAAAGTGACATTGGAAAAAGCTGAGGAAGTTCGAAATTTTAAAGGCTTTAATTATCAGACT
TTTTTGGCTTATCAAGGAATTTACAGGATAGGGAAGGTAGAGCAAATAGAACAGCTTGAAGTGATTTCACCCGAGTCAAT
ATGTGACTATTTGTCCTCTTTAAGACGGCGAGCCATTGTTCATTGCCAGCAGCATTTTCCAAGACGTATGTCTCACTACC
TGACGGGCCTCCTTTTTGGTTATTTAGATAAGTCGTTTGGTGAGATGACAGATTATTATAGTCAGTTGGGTATCATTCAT
CTCTTTGCTTTATCAGGGATGCAGGTTGGCTTCTTTCTCACTTGTTTTAGAAGGGTGTTGCTATTACTCGCAGTTCCTTT
GGAATGGATAAAATGGATAGAGCTTCCTTTTGCTTGCTTTTATGCCGCGTTGACAGGTTATAGTATTTCTGTTATTCGAA
GCCTCGTGCAGTCGCAGTTGCGTCATTTAGGAATAAAAGGATTAGACAATCTCGCTTGTACTTTTTTACTTGTCTTTTTA
TGGGATGCCCATTTCTTAATGACTGTTGGTGGCGTATTAACGTTTAGCTATGCTTTTTTGTTAACAGTGGTTACTGTTGA
AGAATTATCAGGAGCTAAAAGACAGTTGGTACAAGTCTTGACCATTTCCCTAGGAATATTACCATTTTTGCTCTTTTATT
TTTCCAGTTTTAACCCAATGTCAATGGTCTTAACAGCTCTCTTGTCTTATCTCTTTGACCTTTTTATTTTACCTCTTCTT
TGTCTGGTTTTTTGTCTATCCCCTTTGGTGACTGTCTCAATATGCAACCATCTTTTTATTTTATTAGAAAAAGTCATTCA
ATTTCTCGGAAATACTTTCAATAGTTCCCTTGTTTTTGGAAGCCCGACCAGTTGGCACTTGCTTATCTTGGTGATTAGCT
TCGCTATTTTTTATGATTATCGGCAGGTGCGACAACGAGTTATCACTTGCGGTTTGGTTATAGCGTTGACGCTTTTGTCT
GTCAAATATCCTTTGATCAATGAAGTGACCTTTATCGACATTGGCCAGGGAGATAGTATTTTGGTGAGGGAGTGGACAGG
GAAAAATCTCTTAATAGATGTAGGTGGGCGCCCTTTCTTTTCTTCAAAAGAGCACTGGCGACGAGGACATCACGTAGCTA
ATGCTCAGAAAACCTTAATTCCTTACTTAAAAAGTAGAGGTATTCACACAATTGACCAACTATTAGTAACTCACGCAGAT
ACTGATCATATGGGGGATATCGAAGTGGTTGCCAAAGCTATTCGGATAAAGGAGATTTTGACTAGTCAAGGCAGTTTAAG
CCATCCAAGCTTTGTTAGGCGGCTAAGGCGTTTAAAATATCACGTTAGGGTTTTAGCAGCTGGAGACCAGTTGCCTATTA
TGGGAAGTGTCTTACAGGTACTCTACCCTTGGCAGTTAGGGGACGGGAAAAATAACGATTCTTTGGTACTTTATGGCAGG
CTCTTAAATCGAACTTTTCTTTTCACAGGTGACTTGGAAAAAGAGGGGGAAAACGAGATCATAAAACGTTATCCTCAACT
AAGAGTAGATTATTTAAAAGCAGGTCACCATGGATCAAATACCTCTTCTAGTGCAGCTTTTTTAGACCATATTCAGCCTA
AAGTGGCCTTTATTTCTGCTGGAAAAAATAACCGTTATCAGCATCCTCACAGAGAAACCTTAGCCCGTTTGGAAGACAGA
CAAATTACTTATTACCGAACGGATACGCAAGGAGCTATTCGTTTGACTGGTTGGACAAGCTGGCATCTTGAAACGGTCCG
TTAG

Domains

Predicted by InterProScan.

• Competence protein (Competence) (217-462)

• Metallo-beta-lactamase superfamily (Lactamase_B) (495-701)

3D structure

• AlphaFold DB: A0A0H2UUX7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEC/celB	Streptococcus mitis SK321	46.185	100	0.462
comEC/celB	Streptococcus mitis NCTC 12261	45.979	99.866	0.459
comEC/celB	Streptococcus pneumoniae TIGR4	44.98	100	0.45
comEC/celB	Streptococcus pneumoniae Rx1	44.786	100	0.448
comEC/celB	Streptococcus pneumoniae D39	44.786	100	0.448
comEC/celB	Streptococcus pneumoniae R6	44.786	100	0.448
comEC	Lactococcus lactis subsp. cremoris KW2	40.403	99.732	0.403