Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XcfCFBP6996P_RS15810 Genome accession   NZ_CP020989
Coordinates   3752913..3754649 (-) Length   578 a.a.
NCBI ID   WP_099207226.1    Uniprot ID   -
Organism   Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6996R     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3747913..3759649
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XcfCFBP6996P_RS15800 (XcfCFBP6996P_20655) - 3748964..3750577 (+) 1614 WP_007965357.1 HAMP domain-containing sensor histidine kinase -
  XcfCFBP6996P_RS15805 (XcfCFBP6996P_20650) pilR 3750847..3752301 (+) 1455 WP_170922314.1 sigma-54 dependent transcriptional regulator Regulator
  XcfCFBP6996P_RS15810 (XcfCFBP6996P_20645) pilB 3752913..3754649 (-) 1737 WP_099207226.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XcfCFBP6996P_RS15815 (XcfCFBP6996P_20640) - 3754749..3755405 (-) 657 WP_158525796.1 methionine biosynthesis protein MetW -
  XcfCFBP6996P_RS15820 (XcfCFBP6996P_20635) - 3755398..3756444 (-) 1047 WP_099207228.1 glycosyltransferase -
  XcfCFBP6996P_RS15825 (XcfCFBP6996P_20630) - 3756441..3757235 (-) 795 WP_099207229.1 glycosyltransferase family A protein -
  XcfCFBP6996P_RS15830 (XcfCFBP6996P_20625) - 3757239..3757544 (-) 306 WP_234817196.1 SMR family transporter -
  XcfCFBP6996P_RS21840 (XcfCFBP6996P_20620) - 3757920..3758375 (+) 456 WP_269468792.1 pilin -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62600.64 Da        Isoelectric Point: 5.4480

>NTDB_id=227695 XcfCFBP6996P_RS15810 WP_099207226.1 3752913..3754649(-) (pilB) [Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6996R]
MNSVVTANLAGITGIARRLVQDGALDEGAARAAMDQAATAKVPLPQWFSEKKLVTASQLAAANAVEFGMPLLDVSSFDPN
QNAIKLVSDELLQKFQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEAILVDEDQIRRTLEQWQASNASLGSSLG
NDDEEMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=227695 XcfCFBP6996P_RS15810 WP_099207226.1 3752913..3754649(-) (pilB) [Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6996R]
ATGAATAGCGTCGTCACAGCTAACTTGGCTGGTATTACGGGAATCGCCCGGCGTCTTGTGCAGGACGGTGCCTTGGATGA
AGGCGCGGCGCGTGCTGCGATGGATCAGGCCGCTACTGCAAAGGTTCCGTTGCCTCAATGGTTCTCGGAGAAAAAGTTGG
TGACAGCTTCGCAACTTGCGGCGGCCAACGCCGTGGAGTTTGGGATGCCGCTACTTGACGTGTCGTCGTTCGACCCTAAT
CAGAATGCAATTAAATTGGTTAGCGACGAATTGCTGCAAAAGTTTCAAGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTT
GTTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCATACGAACCTGGTGGTCGAAGCCA
TTCTTGTCGACGAGGATCAGATCCGCCGGACGCTCGAACAGTGGCAGGCCAGCAATGCGTCGTTGGGTTCGTCGCTTGGC
AACGACGACGAGGAAATGGGGGATCTGGACGTTTCGGCTGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCGTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCCTGTTGAAGAATGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGTGCGGCCAAGCTGGGCATCGACAAGCTGGGCTATGAGGCAGACCAGCAGAAG
TTGTTCCTGGAGGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGACCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTACAGCAGAACAACAAGCGTGGCATGACCTTCGCGGCAGCCTTGCGCTCGTTCCTGCGACAGGACCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCCCGTTTGATGAACATGGGCATTGCGCCCTACAACATCA
CCTCGTCAGTGACGCTGGTGATCGCGCAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGCTTCCT
GACAACGCATTGCTGGCCGAAGGGTTCACGCCTGCACAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
CGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAAATCGGCGCGATCGTGC
TGGAGGGCGGCAACGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGTTGATG
AAGGCTGCGCACGGGGTGACCAGCCTGGCGGAGATCAATCGAGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

98.27

0.55
  pilB Acinetobacter baylyi ADP1

55.282

98.27

0.543
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilF Neisseria gonorrhoeae MS11

49.472

98.27

0.486
  pilB Vibrio cholerae strain A1552

50

95.848

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.607

96.886

0.452
  pilB Vibrio campbellii strain DS40M4

45.167

98.443

0.445
  pilB/pilB1 Synechocystis sp. PCC 6803

36.453

100

0.384

Multiple sequence alignment