Detailed information NTG0227250

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: MARSALSMR5_RS16845
• Genome accession: NZ_CP020931
• Coordinates: 3680592..3681656 (-)
• Length: 354 a.a.
• NCBI ID: WP_036203576.1
• Uniprot ID: A0A1W6KDI5
• Organism: Marinobacter salarius strain SMR5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3675592..3686656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MARSALSMR5_RS16820 (MARSALSMR5_03418)	aroK	3676023..3676589 (-)	567	WP_036203590.1	shikimate kinase AroK	-
MARSALSMR5_RS16825 (MARSALSMR5_03419)	pilQ	3676719..3678800 (-)	2082	WP_177190642.1	type IV pilus secretin PilQ	Machinery gene
MARSALSMR5_RS16830 (MARSALSMR5_03420)	-	3678867..3679415 (-)	549	WP_036203584.1	pilus assembly protein PilP	-
MARSALSMR5_RS16835 (MARSALSMR5_03421)	-	3679418..3680029 (-)	612	WP_041335800.1	type 4a pilus biogenesis protein PilO	-
MARSALSMR5_RS16840 (MARSALSMR5_03422)	-	3680029..3680592 (-)	564	WP_041335802.1	PilN domain-containing protein	-
MARSALSMR5_RS16845 (MARSALSMR5_03423)	pilM	3680592..3681656 (-)	1065	WP_036203576.1	pilus assembly protein PilM	Machinery gene
MARSALSMR5_RS16850 (MARSALSMR5_03424)	-	3681871..3684357 (+)	2487	WP_036203572.1	penicillin-binding protein 1A	-
MARSALSMR5_RS16855 (MARSALSMR5_03425)	-	3684451..3685716 (-)	1266	WP_036203569.1	malic enzyme-like NAD(P)-binding protein	-
MARSALSMR5_RS16860 (MARSALSMR5_03426)	rpmE	3685949..3686170 (-)	222	WP_007154619.1	50S ribosomal protein L31	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38048.10 Da
• Isoelectric Point: 4.1243

>NTDB_id=227250 MARSALSMR5_RS16845 WP_036203576.1 3680592..3681656(-) (pilM) [Marinobacter salarius strain SMR5]
MFGLLGKKSSSVLGVDISSSSVKLLELSKQGDRYKVESYAVEPLPANAVVEKNITDVEAVGEVLKRVASKSRTSVKQVAV
AVSGSAVITKLIQMDGGLNEFEMEDQITLEADQYIPYPLDEVAIDFEVQGPSEANPDQVDVLLAACRKENVDIREDALEI
ASLTTKIVDVEAYALERAYALIEPQLDSQGEELVVAIVDIGATMTTLSVLAEGKTVYTREQIFGGKQLTEEIQRRYGLSL
EEAGLAKKQGGLPDDYDSEVLTPFREAVVQQVARALQFFFGASQYNAVDYVVLAGGTASIQGLTEMVEEKTGTPTLVANP
FADMAVGSRVNASSLSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=227250 MARSALSMR5_RS16845 WP_036203576.1 3680592..3681656(-) (pilM) [Marinobacter salarius strain SMR5]
GTGTTCGGATTGTTAGGAAAAAAATCCAGTTCGGTGCTGGGCGTGGATATCAGTTCCAGCTCGGTCAAGTTACTGGAACT
ATCGAAGCAGGGCGACCGTTACAAGGTCGAGAGCTACGCAGTGGAGCCGCTACCGGCCAATGCTGTGGTAGAGAAAAACA
TCACGGATGTCGAGGCGGTGGGCGAAGTGCTCAAACGTGTCGCGTCCAAATCACGTACGAGCGTCAAGCAGGTGGCTGTG
GCCGTCTCCGGTTCCGCCGTTATTACCAAGCTGATCCAGATGGACGGTGGTCTTAACGAGTTTGAGATGGAAGACCAGAT
CACGCTCGAGGCCGATCAGTATATTCCCTATCCGTTGGATGAAGTGGCAATCGACTTCGAGGTTCAGGGGCCCTCTGAGG
CTAACCCGGATCAGGTCGATGTACTTCTGGCTGCGTGTCGCAAAGAAAACGTCGATATTCGGGAAGACGCTCTGGAGATT
GCTTCCTTGACCACCAAGATTGTGGACGTCGAAGCATACGCACTCGAGCGGGCATACGCCCTCATCGAGCCTCAGCTTGA
TTCCCAGGGTGAAGAACTCGTTGTGGCGATCGTCGATATCGGCGCAACCATGACCACACTGAGCGTGTTGGCTGAAGGCA
AGACGGTTTACACTCGCGAGCAGATTTTCGGGGGGAAACAACTCACCGAGGAAATTCAGCGTCGTTATGGTCTGTCCCTT
GAAGAGGCCGGCCTTGCCAAGAAGCAGGGTGGCCTCCCCGATGACTACGACTCCGAAGTGCTGACGCCTTTCCGGGAAGC
GGTAGTTCAGCAGGTTGCGAGGGCGCTGCAGTTCTTCTTCGGCGCAAGCCAGTACAATGCGGTTGACTACGTAGTTCTGG
CTGGCGGCACGGCATCTATCCAGGGGCTGACAGAAATGGTCGAGGAAAAAACGGGCACACCCACGCTGGTTGCAAATCCG
TTCGCGGACATGGCAGTGGGATCAAGGGTGAATGCGTCTTCGCTGAGCAATGATGCGCCTTCACTCATGATTGCCTGTGG
GCTGGCAATGAGGAGCTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1W6KDI5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.955	100	0.54
comM	Acinetobacter nosocomialis M2	53.672	100	0.537
comM	Acinetobacter baylyi ADP1	51.695	100	0.517
pilM	Legionella pneumophila strain ERS1305867	45.763	100	0.458