Detailed information NTG0022725

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSPTO_RS15700
• Genome accession: NC_004578
• Coordinates: 3409902..3411071 (-)
• Length: 389 a.a.
• NCBI ID: WP_044389985.1
• Uniprot ID: A0A3M3YY41
• Organism: Pseudomonas syringae pv. tomato str. DC3000
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3404902..3416071

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSPTO_RS15685 (PSPTO3030)	-	3406058..3407122 (+)	1065	WP_011104338.1	microcin C ABC transporter permease YejB	-
PSPTO_RS15690 (PSPTO3031)	-	3407122..3408144 (+)	1023	WP_005764441.1	ABC transporter permease	-
PSPTO_RS15695 (PSPTO3032)	-	3408146..3409723 (+)	1578	WP_011104339.1	ABC transporter ATP-binding protein	-
PSPTO_RS15700 (PSPTO3033)	pilU	3409902..3411071 (-)	1170	WP_044389985.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSPTO_RS15705 (PSPTO3034)	-	3411226..3411501 (+)	276	WP_003373489.1	peptidylprolyl isomerase	-
PSPTO_RS15710 (PSPTO3035)	pgm	3411577..3413223 (-)	1647	WP_011104341.1	phosphoglucomutase (alpha-D-glucose-1,6-bisphosphate-dependent)	-
PSPTO_RS15715 (PSPTO3036)	-	3413324..3415651 (-)	2328	WP_193383848.1	ATP-dependent DNA helicase	-

Sequence

Protein

• Length: 389 a.a.
• Molecular weight: 42668.78 Da
• Isoelectric Point: 5.8663

>NTDB_id=22725 PSPTO_RS15700 WP_044389985.1 3409902..3411071(-) (pilU) [Pseudomonas syringae pv. tomato str. DC3000]
MDFPALLKILASQDGSDLYLSTGAPPCAKFNGVLKPLSSETLKPGEVAVVAQGLMDEEQKLEFVRELEMNLAVSLAGIGR
FRINIFMQRNEVSIVARNIKLDIPRFEDLFLPPVLLDVIMEKHGLVLFVGATGSGKSTSLAALIDYRNRNASGHIITIED
PVEFIHRHKKSIVNQREVGVDTRSFRAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEERRAQLLHDLGNNLKAFVSQRLVKTPDGKRRAAVEVMMGTPTIRDLIQRNELTELKGIMEKSGSLGMQTFDTAL
FNLAVDGAISEEEALKNADSQNNVRLRLKLHSENGVANLTTPPPAPTGSSTASTAEWGLVDDDEPGPQA

Nucleotide

• Length: 1170 bp

>NTDB_id=22725 PSPTO_RS15700 WP_044389985.1 3409902..3411071(-) (pilU) [Pseudomonas syringae pv. tomato str. DC3000]
ATGGATTTTCCGGCGTTGTTGAAGATTCTGGCCAGTCAGGACGGATCTGACCTTTATCTTTCTACCGGAGCACCGCCGTG
TGCCAAGTTCAACGGCGTGCTCAAGCCGCTGAGCAGCGAAACCCTCAAACCTGGCGAAGTGGCGGTGGTTGCCCAGGGGC
TGATGGACGAGGAGCAGAAGCTCGAGTTTGTCCGGGAACTGGAGATGAACCTCGCGGTGTCATTGGCGGGCATCGGCCGG
TTCCGGATCAATATCTTCATGCAGCGCAACGAAGTGTCTATTGTCGCGCGTAACATCAAGCTGGACATTCCGCGCTTTGA
GGACCTTTTCCTGCCGCCGGTGTTGCTTGACGTGATCATGGAAAAACACGGTCTGGTGCTGTTTGTCGGCGCCACCGGCT
CGGGTAAATCGACCTCGCTGGCGGCGCTGATTGATTACCGTAACCGCAATGCCAGCGGGCACATCATCACCATTGAGGAC
CCGGTCGAATTTATCCATCGGCACAAGAAATCCATCGTCAATCAGCGTGAGGTCGGCGTCGACACCCGCAGCTTTCGCGC
GGCACTGAAAAACACTCTGCGTCAGGCCCCGGATGTGATTCTGATCGGCGAGATCCGCGATCGCGAAACGATGGAGCACG
CGCTGGCATTTGCCGACACCGGCCACTTGGCGATTTCGACCCTGCACGCCAATAACGCCAACCAGGCGCTGGACCGAATC
ATCAACTTTTTCCCGGAAGAGCGGCGTGCGCAGCTGTTGCACGACCTGGGCAACAACCTCAAGGCGTTCGTGTCTCAGCG
GCTGGTAAAGACGCCTGATGGAAAACGGCGCGCGGCGGTGGAAGTGATGATGGGCACGCCGACCATCCGCGACCTGATCC
AGCGTAATGAGCTGACTGAACTCAAGGGCATCATGGAAAAGTCCGGCAGCCTCGGGATGCAGACGTTCGATACCGCGCTG
TTCAACCTGGCCGTCGATGGCGCGATCAGCGAGGAAGAAGCGTTGAAAAATGCCGATTCGCAAAACAACGTTCGTCTGCG
CCTCAAGCTGCACAGCGAGAACGGAGTGGCTAACCTGACCACGCCGCCCCCGGCACCGACCGGCAGCAGCACAGCCAGCA
CCGCCGAGTGGGGCCTGGTCGACGATGACGAGCCGGGGCCGCAGGCGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (115-248)

3D structure

• AlphaFold DB: A0A3M3YY41

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.072	99.486	0.558
pilU	Acinetobacter baylyi ADP1	52.113	91.26	0.476
pilU	Vibrio cholerae strain A1552	51.429	89.974	0.463
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.164	98.458	0.386
pilT	Pseudomonas aeruginosa PAK	41.399	88.175	0.365