Detailed information NTG0224349

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B7L45_RS01875
• Genome accession: NZ_CP020579
• Coordinates: 394972..396030 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain SAA14
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 389972..401030

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B7L45_RS01860 (B7L45_01860)	-	391077..391409 (+)	333	WP_000169594.1	hypothetical protein	-
B7L45_RS01865 (B7L45_01865)	-	391412..392236 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
B7L45_RS01870 (B7L45_01870)	ponA	392254..394809 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
B7L45_RS01875 (B7L45_01875)	pilM	394972..396030 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
B7L45_RS01880 (B7L45_01880)	pilN	396030..396671 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
B7L45_RS01885 (B7L45_01885)	pilO	396668..397408 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
B7L45_RS01890 (B7L45_01890)	pilP	397419..397946 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
B7L45_RS01895 (B7L45_01895)	pilQ	398014..400176 (+)	2163	WP_033503048.1	type IV pilus secretin PilQ family protein	Machinery gene
B7L45_RS01900 (B7L45_01900)	aroK	400188..400757 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=224349 B7L45_RS01875 WP_002014066.1 394972..396030(+) (pilM) [Acinetobacter baumannii strain SAA14]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=224349 B7L45_RS01875 WP_002014066.1 394972..396030(+) (pilM) [Acinetobacter baumannii strain SAA14]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423