Detailed information NTG0224280

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: B6S64_RS13260
• Genome accession: NZ_CP020574
• Coordinates: 2658209..2659267 (+)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain 15A5
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2653209..2664267

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B6S64_RS13245 (B6S64_13245)	-	2654314..2654646 (+)	333	WP_000169594.1	hypothetical protein	-
B6S64_RS13250 (B6S64_13250)	-	2654649..2655473 (+)	825	WP_002019666.1	putative RNA methyltransferase	-
B6S64_RS13255 (B6S64_13255)	ponA	2655491..2658046 (-)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
B6S64_RS13260 (B6S64_13260)	pilM	2658209..2659267 (+)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
B6S64_RS13265 (B6S64_13265)	pilN	2659267..2659908 (+)	642	WP_000201227.1	PilN domain-containing protein	Machinery gene
B6S64_RS13270 (B6S64_13270)	pilO	2659905..2660645 (+)	741	WP_000076101.1	type 4a pilus biogenesis protein PilO	Machinery gene
B6S64_RS13275 (B6S64_13275)	pilP	2660656..2661183 (+)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
B6S64_RS13280 (B6S64_13280)	pilQ	2661251..2663413 (+)	2163	WP_033503048.1	type IV pilus secretin PilQ family protein	Machinery gene
B6S64_RS13285 (B6S64_13285)	aroK	2663425..2663994 (+)	570	WP_000643712.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=224280 B6S64_RS13260 WP_002014066.1 2658209..2659267(+) (pilM) [Acinetobacter baumannii strain 15A5]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=224280 B6S64_RS13260 WP_002014066.1 2658209..2659267(+) (pilM) [Acinetobacter baumannii strain 15A5]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCCGATCGTCTTG
CGAATCCAAATCGTGTAAATGTACTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGCCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTCTTTGGTGGCAAGCAACTTACGCTTGAAATTCAAAGTCGTTATGGCTTGTCTTTAGAAGAA
GCAAGCCGAGCGAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTAGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCCAAGCTTTTGCAGCAAAAATTAGGTTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423