Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   B6S64_RS13270 Genome accession   NZ_CP020574
Coordinates   2659905..2660645 (+) Length   246 a.a.
NCBI ID   WP_000076101.1    Uniprot ID   N9LH62
Organism   Acinetobacter baumannii strain 15A5     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2654905..2665645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6S64_RS13255 (B6S64_13255) ponA 2655491..2658046 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  B6S64_RS13260 (B6S64_13260) pilM 2658209..2659267 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  B6S64_RS13265 (B6S64_13265) pilN 2659267..2659908 (+) 642 WP_000201227.1 PilN domain-containing protein Machinery gene
  B6S64_RS13270 (B6S64_13270) pilO 2659905..2660645 (+) 741 WP_000076101.1 type 4a pilus biogenesis protein PilO Machinery gene
  B6S64_RS13275 (B6S64_13275) pilP 2660656..2661183 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  B6S64_RS13280 (B6S64_13280) pilQ 2661251..2663413 (+) 2163 WP_033503048.1 type IV pilus secretin PilQ family protein Machinery gene
  B6S64_RS13285 (B6S64_13285) aroK 2663425..2663994 (+) 570 WP_000643712.1 shikimate kinase AroK -
  B6S64_RS13290 (B6S64_13290) aroB 2664023..2665105 (+) 1083 WP_001193997.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27819.59 Da        Isoelectric Point: 4.8357

>NTDB_id=224282 B6S64_RS13270 WP_000076101.1 2659905..2660645(+) (pilO) [Acinetobacter baumannii strain 15A5]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGATDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=224282 B6S64_RS13270 WP_000076101.1 2659905..2660645(+) (pilO) [Acinetobacter baumannii strain 15A5]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTACGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGCATCGCTGCTTTGCCTCGTATTGTAAC
CATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGGCAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCTTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB N9LH62

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

100

100

1
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683

Multiple sequence alignment