Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B6A39_RS09505 Genome accession   NZ_CP020562
Coordinates   2025436..2027190 (+) Length   584 a.a.
NCBI ID   WP_083004560.1    Uniprot ID   -
Organism   Halomonas sp. GT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2020436..2032190
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6A39_RS09470 - 2020991..2022340 (+) 1350 WP_083004540.1 site-specific integrase -
  B6A39_RS09475 - 2022384..2022602 (-) 219 WP_083004544.1 hypothetical protein -
  B6A39_RS09490 - 2023275..2024084 (-) 810 WP_083004551.1 inositol monophosphatase family protein -
  B6A39_RS09495 - 2024180..2024635 (-) 456 WP_083004554.1 anti-virulence regulator CigR family protein -
  B6A39_RS19140 - 2024769..2025191 (-) 423 WP_083004557.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B6A39_RS09505 pilB 2025436..2027190 (+) 1755 WP_083004560.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B6A39_RS09510 - 2027199..2028428 (+) 1230 WP_083004564.1 type II secretion system F family protein -
  B6A39_RS09515 pilD 2028490..2029335 (+) 846 WP_083004567.1 A24 family peptidase Machinery gene
  B6A39_RS09520 coaE 2029375..2029971 (+) 597 WP_083004570.1 dephospho-CoA kinase -
  B6A39_RS09525 yacG 2030080..2030313 (+) 234 WP_083004574.1 DNA gyrase inhibitor YacG -
  B6A39_RS09530 - 2030490..2031362 (+) 873 WP_083004577.1 DNA replication terminus site-binding protein -
  B6A39_RS09535 - 2031390..2031737 (-) 348 WP_083004581.1 hypothetical protein -

Sequence

Protein


Download         Length: 584 a.a.        Molecular weight: 63683.05 Da        Isoelectric Point: 6.5091

>NTDB_id=224152 B6A39_RS09505 WP_083004560.1 2025436..2027190(+) (pilB) [Halomonas sp. GT]
MSQSAPESTLSHATLHGGLRGIAKRLVKHGVLTDAQATVAESTAAELEVSLLQHVVDSGLVESGAAALAAAWEYGLPVVD
LDAIRINALPPANDYPVKILERLDILPLARHGHRLTVAVPYPATLTQLDELQFATGLSVDGVLAPADQLKNTLTQYLAQN
ERSMLDELENVDDAVSALNIVQLGEDEATFDKAVKDSEDAPVVKFVNKILLDAISRGASDIHFEPYETQYRVRFRIDGML
IEIARPPFALRHRIAARLKIMSRLDISERRLPQDGAIKLQLSRTRSIDFRVNSLPTVFGEKIVLRLLDPASAQLGIEQLG
FSPEQQAAYEHALKKPQGMILVTGPTGSGKTVTLYTGINILNQIERNICTAEDPVEIKVSGINQVNVLPKIGLNFASALR
AFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVASYNIASSVSLIIAQRLARKLCNLC
KTPAKIPAEALRKAGFSQQDIRNGTIYSPVGCKQCTQGYKGRIGIYEVVPITDAMRQLIMRDGDALDIDQQARREGYPSL
HQRCLSRVLDGSTSLEEVNRISKE

Nucleotide


Download         Length: 1755 bp        

>NTDB_id=224152 B6A39_RS09505 WP_083004560.1 2025436..2027190(+) (pilB) [Halomonas sp. GT]
ATGAGCCAATCTGCCCCAGAGTCTACGCTGAGCCATGCGACCTTACATGGCGGCCTGCGCGGTATCGCTAAACGGCTGGT
GAAACATGGCGTGCTGACCGATGCTCAGGCGACTGTCGCGGAAAGCACGGCAGCAGAGCTTGAAGTGTCGCTGTTACAGC
ATGTGGTCGATAGTGGGCTAGTAGAGTCAGGCGCAGCAGCACTGGCCGCTGCCTGGGAGTATGGTTTACCTGTTGTTGAC
CTAGATGCCATCCGTATTAATGCACTGCCTCCTGCCAACGACTACCCCGTTAAAATATTAGAGCGCCTGGATATTCTTCC
CCTGGCACGCCACGGGCATCGTCTAACTGTTGCGGTGCCCTACCCCGCTACGCTTACCCAGCTGGATGAGCTGCAATTTG
CCACTGGGCTAAGCGTAGATGGCGTACTGGCGCCCGCTGACCAACTTAAAAACACCCTAACGCAGTATCTGGCGCAAAAT
GAACGCAGCATGCTGGATGAGCTGGAAAACGTCGATGACGCCGTCAGCGCGCTGAATATTGTTCAGCTTGGCGAGGATGA
GGCGACCTTTGATAAAGCGGTAAAAGATAGCGAAGACGCCCCTGTCGTTAAATTCGTTAATAAAATACTGTTAGATGCCA
TCAGTCGTGGCGCTTCCGATATTCACTTTGAGCCTTATGAAACCCAGTACCGGGTGCGCTTTCGCATTGATGGCATGCTA
ATTGAAATTGCTCGTCCGCCTTTTGCGTTACGTCACCGTATTGCGGCACGTTTGAAAATCATGTCTCGGCTGGATATTTC
TGAACGGCGACTGCCTCAGGATGGCGCGATCAAGTTACAGCTTTCACGCACCCGTTCAATAGATTTTCGGGTGAACTCAC
TGCCCACCGTATTCGGTGAAAAAATAGTATTACGCCTGTTAGACCCGGCCTCTGCCCAGCTTGGTATCGAACAACTAGGC
TTTAGCCCGGAACAACAAGCCGCCTACGAACATGCGCTAAAAAAACCCCAAGGCATGATTTTAGTTACCGGGCCGACGGG
TAGCGGTAAAACAGTGACGCTTTACACAGGTATTAATATTCTCAACCAAATCGAGCGCAATATCTGTACCGCTGAAGACC
CGGTAGAAATTAAAGTGTCCGGCATCAATCAGGTTAACGTTCTCCCTAAAATTGGCTTGAACTTCGCTAGTGCTTTGCGC
GCCTTTCTACGCCAAGACCCTGACGTGGTGATGGTAGGTGAAATTCGTGACCTGGAAACCGCCGAAATTGCCGTAAAGGC
AGCCCAAACAGGCCACTTAGTGCTATCTACGGTACATACTAACTCAGCTGCTGAAACGCTAACCCGGTTAGCGAATATGG
GCGTCGCCTCCTATAACATTGCAAGCTCCGTTAGCTTGATCATTGCCCAGCGCTTGGCGCGTAAGCTGTGCAACCTGTGT
AAAACACCCGCTAAGATTCCGGCCGAAGCGCTGCGTAAGGCTGGCTTTAGCCAGCAGGATATTCGCAATGGCACAATTTA
TTCGCCAGTTGGCTGCAAACAGTGCACCCAGGGCTACAAGGGGCGTATTGGTATTTATGAAGTAGTGCCCATTACCGATG
CCATGCGCCAATTGATTATGCGTGATGGTGATGCGCTGGATATTGATCAGCAAGCCCGCCGCGAAGGCTACCCCAGCCTT
CATCAGCGTTGCTTGAGTCGCGTACTGGATGGCAGTACCAGCTTGGAAGAAGTTAACCGCATCAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-161)

Type II/IV secretion system protein (T2SSE)

(208-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

52.091

98.288

0.512
  pilB Acinetobacter baylyi ADP1

49.74

98.801

0.491
  pilB Acinetobacter baumannii D1279779

49.823

96.918

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

48.336

97.774

0.473
  pilB Vibrio campbellii strain DS40M4

47.719

97.603

0.466
  pilB Vibrio cholerae strain A1552

51.852

87.842

0.455
  pilF Neisseria gonorrhoeae MS11

49.476

81.678

0.404
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.487

100

0.392

Multiple sequence alignment