Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   B6A39_RS09515 Genome accession   NZ_CP020562
Coordinates   2028490..2029335 (+) Length   281 a.a.
NCBI ID   WP_083004567.1    Uniprot ID   -
Organism   Halomonas sp. GT     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2023490..2034335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6A39_RS09495 - 2024180..2024635 (-) 456 WP_083004554.1 anti-virulence regulator CigR family protein -
  B6A39_RS19140 - 2024769..2025191 (-) 423 WP_083004557.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B6A39_RS09505 pilB 2025436..2027190 (+) 1755 WP_083004560.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B6A39_RS09510 - 2027199..2028428 (+) 1230 WP_083004564.1 type II secretion system F family protein -
  B6A39_RS09515 pilD 2028490..2029335 (+) 846 WP_083004567.1 A24 family peptidase Machinery gene
  B6A39_RS09520 coaE 2029375..2029971 (+) 597 WP_083004570.1 dephospho-CoA kinase -
  B6A39_RS09525 yacG 2030080..2030313 (+) 234 WP_083004574.1 DNA gyrase inhibitor YacG -
  B6A39_RS09530 - 2030490..2031362 (+) 873 WP_083004577.1 DNA replication terminus site-binding protein -
  B6A39_RS09535 - 2031390..2031737 (-) 348 WP_083004581.1 hypothetical protein -
  B6A39_RS09540 - 2032108..2032941 (+) 834 WP_083004584.1 helix-turn-helix transcriptional regulator -
  B6A39_RS09545 - 2033019..2033393 (-) 375 WP_009724762.1 histone-like nucleoid-structuring protein, MvaT/MvaU family -

Sequence

Protein


Download         Length: 281 a.a.        Molecular weight: 30902.31 Da        Isoelectric Point: 8.4767

>NTDB_id=224153 B6A39_RS09515 WP_083004567.1 2028490..2029335(+) (pilD) [Halomonas sp. GT]
MGLPPSFYLFFALLIGLCLGSFLNVVITRLPVMLMRRWRNEARVSLELTEETSPRFNLATPRSMCPRCEQPIVWHDNLPL
IGWLKRCGKCAHCQTPISVQYPLVELMGGVLAVTIVALHGPTLSALFIYGACLTLLALAVIDFRTYLLPDMLTLPLLWAG
LAYQLFFQPLLLPSAVIGAMAGYLVLWTFYWVFKLITGKEGMGFGDFKLLAALGAWVGWSMLPLVLILSAGIGAIVGLAA
QALSPRLRGQPLPFGPFLALAGWICLLVGNELMALYTSLLY

Nucleotide


Download         Length: 846 bp        

>NTDB_id=224153 B6A39_RS09515 WP_083004567.1 2028490..2029335(+) (pilD) [Halomonas sp. GT]
ATGGGTTTACCGCCAAGCTTTTACCTCTTTTTTGCTTTACTGATCGGCCTCTGCTTGGGCAGTTTTCTTAACGTTGTCAT
CACTCGTTTACCCGTTATGCTGATGCGCCGTTGGCGCAATGAAGCACGCGTCTCTCTTGAGCTAACCGAAGAAACGTCTC
CCCGCTTTAACCTTGCCACTCCAAGGTCAATGTGCCCACGCTGTGAACAACCGATTGTTTGGCACGATAACCTACCCTTA
ATAGGCTGGCTAAAAAGATGTGGCAAATGTGCCCATTGTCAAACACCCATCAGTGTACAGTACCCACTCGTTGAGCTAAT
GGGCGGCGTGCTAGCAGTCACGATTGTGGCTTTGCATGGCCCGACGCTAAGCGCACTGTTTATCTATGGCGCTTGTTTAA
CGCTACTTGCTTTGGCAGTGATCGATTTTCGTACCTATTTACTCCCTGATATGTTGACCCTACCGTTGTTGTGGGCTGGC
TTAGCCTATCAATTGTTCTTTCAGCCTCTGCTGCTCCCCAGTGCTGTTATCGGCGCGATGGCAGGCTATCTTGTGCTTTG
GACCTTTTACTGGGTATTTAAGCTCATAACAGGTAAAGAAGGCATGGGATTTGGGGATTTCAAATTACTGGCCGCCCTTG
GCGCTTGGGTAGGTTGGAGCATGTTACCGCTGGTGCTTATTTTATCAGCAGGTATTGGTGCAATAGTAGGACTAGCCGCT
CAGGCTCTATCTCCTCGACTGCGTGGCCAACCTCTTCCCTTTGGCCCCTTTCTTGCGCTAGCGGGCTGGATTTGTTTACT
TGTGGGCAACGAGTTAATGGCACTCTACACGTCGTTGCTTTATTAA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(14-116)

Type IV leader peptidase family (Peptidase_A24)

(132-238)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

49.275

98.221

0.484
  pilD Vibrio campbellii strain DS40M4

45.714

99.644

0.456
  pilD Acinetobacter baumannii D1279779

47.547

94.306

0.448
  pilD Acinetobacter nosocomialis M2

47.547

94.306

0.448
  pilD Neisseria gonorrhoeae MS11

44.815

96.085

0.431

Multiple sequence alignment