Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   C2H94_RS03225 Genome accession   NZ_CP026034
Coordinates   548814..549818 (-) Length   334 a.a.
NCBI ID   WP_003229718.1    Uniprot ID   O32055
Organism   Bacillus subtilis strain PK5_52     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 543814..554818
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C2H94_RS03195 (C2H94_03155) yrbG 544788..545444 (-) 657 WP_021480225.1 DUF421 domain-containing protein -
  C2H94_RS03200 (C2H94_03160) - 545642..546031 (+) 390 WP_031315243.1 TIGR04086 family membrane protein -
  C2H94_RS03205 (C2H94_03165) yajC 546088..546354 (-) 267 WP_004398708.1 preprotein translocase subunit YajC -
  C2H94_RS03210 (C2H94_03170) tgt 546391..547536 (-) 1146 WP_003229725.1 tRNA guanosine(34) transglycosylase Tgt -
  C2H94_RS03215 (C2H94_03175) queA 547563..548591 (-) 1029 WP_003229723.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  C2H94_RS03220 (C2H94_03180) yrzS 548621..548821 (-) 201 WP_003222669.1 DUF2905 domain-containing protein -
  C2H94_RS03225 (C2H94_03185) ruvB 548814..549818 (-) 1005 WP_003229718.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  C2H94_RS03230 (C2H94_03190) ruvA 549829..550434 (-) 606 WP_003229717.1 Holliday junction branch migration protein RuvA Machinery gene
  C2H94_RS03235 (C2H94_03195) bofC 550573..551085 (-) 513 WP_003229715.1 sporulation cell-cell signaling protein BofC -
  C2H94_RS03240 (C2H94_03200) csbX 551133..552440 (-) 1308 WP_038829637.1 MFS transporter -
  C2H94_RS03245 (C2H94_03205) iolG 552511..553536 (-) 1026 WP_070547599.1 inositol 2-dehydrogenase -
  C2H94_RS03250 (C2H94_03210) yrzF 553774..554421 (+) 648 WP_024572252.1 phosphotransferase -
  C2H94_RS03255 - 554467..554589 (-) 123 WP_003229707.1 hypothetical protein -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37467.78 Da        Isoelectric Point: 5.1631

>NTDB_id=222163 C2H94_RS03225 WP_003229718.1 548814..549818(-) (ruvB) [Bacillus subtilis strain PK5_52]
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=222163 C2H94_RS03225 WP_003229718.1 548814..549818(-) (ruvB) [Bacillus subtilis strain PK5_52]
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCTATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGGCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTCATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGTCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB O32055

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

100

100

1
  ruvB Streptococcus pneumoniae TIGR4

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae R6

60.429

97.605

0.59
  ruvB Streptococcus pneumoniae D39

60.429

97.605

0.59
  ruvB Synechocystis sp. PCC 6803

57.143

98.503

0.563
  ruvB Helicobacter pylori 26695

52.744

98.204

0.518