Detailed information    

insolico Bioinformatically predicted

Overview

Name   ruvB   Type   Machinery gene
Locus tag   SAS_RS08365 Genome accession   NC_002953
Coordinates   1701420..1702424 (-) Length   334 a.a.
NCBI ID   WP_001005768.1    Uniprot ID   P66757
Organism   Staphylococcus aureus subsp. aureus MSSA476     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1696420..1707424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAS_RS08350 (SAS1574) yajC 1698952..1699212 (-) 261 WP_001160682.1 preprotein translocase subunit YajC -
  SAS_RS08355 (SAS1575) tgt 1699231..1700370 (-) 1140 WP_001112045.1 tRNA guanosine(34) transglycosylase Tgt -
  SAS_RS08360 (SAS1576) queA 1700393..1701418 (-) 1026 WP_001019178.1 tRNA preQ1(34) S-adenosylmethionine ribosyltransferase-isomerase QueA -
  SAS_RS08365 (SAS1577) ruvB 1701420..1702424 (-) 1005 WP_001005768.1 Holliday junction branch migration DNA helicase RuvB Machinery gene
  SAS_RS08370 (SAS1578) ruvA 1702456..1703058 (-) 603 WP_000271550.1 Holliday junction branch migration protein RuvA Machinery gene
  SAS_RS08375 (SAS1579) - 1703072..1703527 (-) 456 WP_000368902.1 ACT domain-containing protein -
  SAS_RS08380 (SAS1580) obgE 1703540..1704832 (-) 1293 WP_000496100.1 GTPase ObgE -
  SAS_RS08385 (SAS1581) rpmA 1705317..1705601 (-) 285 WP_000916187.1 50S ribosomal protein L27 -
  SAS_RS08390 (SAS1582) - 1705613..1705933 (-) 321 WP_000633692.1 ribosomal-processing cysteine protease Prp -
  SAS_RS08395 (SAS1583) rplU 1705939..1706247 (-) 309 WP_000457386.1 50S ribosomal protein L21 -
  SAS_RS08400 (SAS1584) mreD 1706609..1707139 (-) 531 WP_001259925.1 rod shape-determining protein MreD -

Sequence

Protein


Download         Length: 334 a.a.        Molecular weight: 37645.94 Da        Isoelectric Point: 5.1876

>NTDB_id=22163 SAS_RS08365 WP_001005768.1 1701420..1702424(-) (ruvB) [Staphylococcus aureus subsp. aureus MSSA476]
MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIKAAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVN
IRTVSGPSLERPGDLAAILSGLQPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTPRVANRLLKRVRDFQQVNEDEQI
YIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNGGPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATP
LAYEHFAKSNEERG

Nucleotide


Download         Length: 1005 bp        

>NTDB_id=22163 SAS_RS08365 WP_001005768.1 1701420..1702424(-) (ruvB) [Staphylococcus aureus subsp. aureus MSSA476]
ATGAATGAGCGTATGGTTGATCAATCAATGCATAGTGAAGAAACTGATTTCGAATTGTCGCTTAGACCTACGAGATTACG
ACAATATATTGGTCAAAATTCAATAAAAAGTAATTTAGAAGTATTTATTAAAGCGGCTAAACTTCGTCATGAACCATTAG
ATCATGTATTGCTTTTTGGCCCCCCTGGATTAGGTAAGACAACATTATCTAATATCATTGCCAATGAAATGGAAGTTAAT
ATACGTACAGTATCAGGGCCTTCATTAGAAAGACCTGGTGATTTGGCTGCAATTTTATCAGGACTTCAACCTGGAGATGT
ATTGTTTATTGATGAAATACACAGACTGAGTAGTGTTGTTGAAGAAGTGTTATACCCTGCAATGGAAGATTTCTTTTTAG
ATATTATCATTGGTAAAGGCGATGAGGCTAGAAGTATCCGTATCGACTTACCTCCATTCACTTTGGTAGGTGCAACAACG
CGAGCTGGCAGCTTAACAGGTCCACTAAGGGATCGATTTGGTGTGCACTTAAGATTAGAATATTATAATGAATCAGATTT
AAAAGAAATCATTATTAGAACAGCTGAGGTTTTAGGCACAGGTATTGATGAAGAAAGTGCCATTGAACTTGCTAAACGTT
CTAGAGGGACTCCAAGAGTAGCAAATCGACTATTGAAGCGGGTAAGAGACTTCCAGCAAGTGAATGAAGATGAACAAATA
TACATTGAAACAACGAAGCACGCATTAGGTTTACTTCAAGTTGATCAACACGGACTAGATTACATTGATCATAAAATGAT
GAACTGTATTATTAAGCAGTATAATGGTGGACCTGTTGGTTTAGATACGATTGCCGTAACAATTGGTGAAGAACGTATTA
CAATTGAGGACGTTTATGAGCCATTTCTTATTCAGAAAGGCTTTTTAGAACGTACGCCACGTGGCAGAAAAGCAACACCA
TTAGCTTATGAACATTTTGCAAAGTCGAATGAGGAGAGAGGATAA

Domains

Predicted by InterProScan.

RuvB AAA lid domain (AAA_lid_4)

(182-255)

RuvB C-terminal winged helix domain (WHD_RuvB)

(257-326)

Holliday junction DNA helicase RuvB P-loop domain (RuvB_N)

(21-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P66757

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ruvB Bacillus subtilis subsp. subtilis str. 168

65.951

97.605

0.644
  ruvB Streptococcus pneumoniae TIGR4

58.182

98.802

0.575
  ruvB Streptococcus pneumoniae R6

57.879

98.802

0.572
  ruvB Streptococcus pneumoniae D39

57.879

98.802

0.572
  ruvB Helicobacter pylori 26695

56.481

97.006

0.548
  ruvB Synechocystis sp. PCC 6803

53.75

95.808

0.515

Multiple sequence alignment