Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   BVH74_RS06700 Genome accession   NZ_CP020100
Coordinates   1449161..1450225 (+) Length   354 a.a.
NCBI ID   WP_080049313.1    Uniprot ID   A0A396S845
Organism   Halopseudomonas phragmitis strain S-6-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1444161..1455225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVH74_RS06690 (BVH74_06690) - 1445658..1446575 (+) 918 WP_080049311.1 LysR family transcriptional regulator -
  BVH74_RS06695 (BVH74_06695) - 1446572..1448974 (-) 2403 WP_080049312.1 penicillin-binding protein 1A -
  BVH74_RS06700 (BVH74_06700) pilM 1449161..1450225 (+) 1065 WP_080049313.1 pilus assembly protein PilM Machinery gene
  BVH74_RS06705 (BVH74_06705) - 1450225..1450803 (+) 579 WP_080049314.1 PilN domain-containing protein -
  BVH74_RS06710 (BVH74_06710) - 1450800..1451429 (+) 630 WP_080049315.1 type 4a pilus biogenesis protein PilO -
  BVH74_RS06715 (BVH74_06715) - 1451432..1451956 (+) 525 WP_080051647.1 pilus assembly protein PilP -
  BVH74_RS06720 (BVH74_06720) pilQ 1452016..1454142 (+) 2127 WP_080051648.1 type IV pilus secretin PilQ Machinery gene
  BVH74_RS06725 (BVH74_06725) aroK 1454148..1454666 (+) 519 WP_080049316.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38113.79 Da        Isoelectric Point: 4.3903

>NTDB_id=221086 BVH74_RS06700 WP_080049313.1 1449161..1450225(+) (pilM) [Halopseudomonas phragmitis strain S-6-2]
MLGLLKKKANTLLGIDISSTTVKLLELSRSGNRYKVEAYAVEPLPPGAVVEKNIVELDGVGQALEKVLARSKSPLKQAAV
AVAGSAVITKSIELDGGLTPEEMEEQIKLEADQYIPYPLDEVAIDFEVQGPAPRNPDRVEVLLAACRRENVDIREAALAL
GGMAAKVVEVEAYAMERACELVIDQLEGDRDDLTIAVIDIGATMMTLSVLNKGRIIYTREQLFGGKQLTDEIQRRYGLSL
EEAGLAKKQGGLPDDYESEVLGPFKDAVVQQVSRSLQFFFAGGQYNDVDYILLAGGTASIPGLDQLIQQKIGTTTLVANP
FANMTLSNKVNAVALSNDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=221086 BVH74_RS06700 WP_080049313.1 1449161..1450225(+) (pilM) [Halopseudomonas phragmitis strain S-6-2]
GTGCTAGGGCTCCTAAAAAAGAAAGCAAATACGCTGCTGGGCATAGACATCAGTTCCACAACCGTGAAGCTGTTGGAGCT
CAGTCGTTCAGGTAACCGCTACAAGGTCGAGGCCTACGCGGTTGAGCCACTCCCTCCAGGGGCGGTGGTGGAGAAGAATA
TTGTTGAGCTGGATGGGGTGGGGCAGGCGCTGGAAAAGGTCCTGGCACGCTCGAAAAGTCCACTCAAGCAGGCGGCTGTG
GCTGTCGCCGGCTCCGCCGTGATCACCAAGTCCATCGAGCTTGATGGCGGCCTTACTCCCGAAGAGATGGAAGAGCAGAT
CAAGCTCGAGGCCGACCAGTACATTCCTTATCCGCTGGATGAAGTGGCGATTGACTTCGAAGTTCAGGGGCCGGCGCCAC
GCAACCCCGATCGGGTTGAAGTGTTGCTGGCAGCCTGCCGACGGGAAAATGTCGATATTCGCGAGGCTGCGCTGGCACTG
GGCGGGATGGCTGCCAAGGTGGTCGAGGTCGAAGCTTATGCTATGGAACGAGCCTGCGAGCTGGTCATCGACCAACTGGA
GGGTGACCGGGATGATCTGACCATCGCAGTGATCGATATCGGCGCCACCATGATGACCCTCAGTGTTCTCAACAAGGGTC
GCATCATATACACCCGCGAGCAGTTGTTCGGCGGCAAGCAACTTACCGACGAAATCCAGCGTCGCTATGGATTGTCGCTG
GAAGAAGCCGGGTTGGCGAAAAAGCAGGGCGGACTACCTGACGACTATGAGAGCGAGGTTTTAGGTCCGTTCAAGGACGC
TGTCGTGCAGCAGGTCTCTCGTTCGCTGCAGTTCTTCTTTGCCGGCGGTCAGTACAACGACGTCGACTATATCCTGCTGG
CTGGCGGCACCGCCTCGATTCCCGGGCTCGATCAACTGATTCAGCAGAAGATCGGCACCACAACGCTGGTGGCTAACCCC
TTTGCAAACATGACCCTCTCGAACAAGGTCAATGCCGTTGCCCTGAGCAATGATGCGCCTGCCTTGATGATTGCATGCGG
TCTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A396S845

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

55.085

100

0.551
  comM Acinetobacter nosocomialis M2

55.085

100

0.551
  comM Acinetobacter baylyi ADP1

52.542

100

0.525
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466

Multiple sequence alignment