Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   B4W81_RS03065 Genome accession   NZ_CP019992
Coordinates   595104..596393 (+) Length   429 a.a.
NCBI ID   WP_002294152.1    Uniprot ID   A0AAV3GR07
Organism   Enterococcus faecium isolate 2014-VREF-268     
Function   repress competence development (predicted from homology)   
Competence regulation

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 555001..594676 595104..596393 flank 428


Gene organization within MGE regions


Location: 555001..596393
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4W81_RS02860 (B4W81_02860) - 556232..556438 (-) 207 WP_010706118.1 hypothetical protein -
  B4W81_RS02865 (B4W81_02865) - 556601..558235 (-) 1635 WP_077974435.1 recombinase family protein -
  B4W81_RS02870 (B4W81_02870) - 558239..559612 (-) 1374 WP_002312624.1 recombinase family protein -
  B4W81_RS02875 (B4W81_02875) - 559628..559867 (-) 240 WP_002312623.1 hypothetical protein -
  B4W81_RS02880 (B4W81_02880) - 560049..562724 (-) 2676 WP_002323671.1 DUF927 domain-containing protein -
  B4W81_RS02885 (B4W81_02885) - 562907..563830 (-) 924 WP_002312619.1 hypothetical protein -
  B4W81_RS02890 (B4W81_02890) - 564274..565353 (+) 1080 WP_077974289.1 IS30 family transposase -
  B4W81_RS02895 (B4W81_02895) - 565681..566640 (-) 960 WP_002301399.1 IS30 family transposase -
  B4W81_RS02900 (B4W81_02900) - 566811..567815 (-) 1005 WP_002312618.1 DUF4767 domain-containing protein -
  B4W81_RS02905 (B4W81_02905) - 567886..568182 (-) 297 WP_002312617.1 hypothetical protein -
  B4W81_RS02910 (B4W81_02910) - 568175..568468 (-) 294 WP_002296258.1 Mor transcription activator family protein -
  B4W81_RS02915 (B4W81_02915) - 568614..569165 (-) 552 WP_002312616.1 DUF1643 domain-containing protein -
  B4W81_RS02920 (B4W81_02920) - 569339..569638 (-) 300 WP_002312614.1 hypothetical protein -
  B4W81_RS02925 (B4W81_02925) - 569665..569973 (-) 309 WP_002323668.1 DUF960 domain-containing protein -
  B4W81_RS02930 (B4W81_02930) - 570090..571049 (-) 960 WP_002312610.1 IS30 family transposase -
  B4W81_RS02935 (B4W81_02935) - 571193..571969 (+) 777 WP_002312609.1 GntR family transcriptional regulator -
  B4W81_RS02940 (B4W81_02940) - 572067..572714 (-) 648 WP_002298781.1 HAD family hydrolase -
  B4W81_RS02945 (B4W81_02945) - 572714..573544 (-) 831 WP_002312607.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
  B4W81_RS02950 (B4W81_02950) - 573544..574293 (-) 750 WP_002298779.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
  B4W81_RS02955 (B4W81_02955) - 574307..574771 (-) 465 WP_002298777.1 PTS sugar transporter subunit IIB -
  B4W81_RS02960 (B4W81_02960) - 574821..575282 (-) 462 WP_002312606.1 PTS sugar transporter subunit IIA -
  B4W81_RS02965 (B4W81_02965) - 575264..576238 (-) 975 WP_002298774.1 SIS domain-containing protein -
  B4W81_RS02970 (B4W81_02970) - 576374..577333 (-) 960 WP_002301399.1 IS30 family transposase -
  B4W81_RS02975 (B4W81_02975) - 577511..578974 (+) 1464 Protein_557 IS1182-like element ISEfa7 family transposase -
  B4W81_RS02980 (B4W81_02980) - 579098..579433 (+) 336 Protein_558 transposase -
  B4W81_RS02985 (B4W81_02985) - 579529..580690 (+) 1162 Protein_559 IS3 family transposase -
  B4W81_RS02990 (B4W81_02990) - 580674..581267 (-) 594 Protein_560 IbrB-like domain-containing protein -
  B4W81_RS02995 (B4W81_02995) - 581660..583100 (+) 1441 Protein_561 DNA-binding protein -
  B4W81_RS03000 (B4W81_03000) - 583265..584665 (-) 1401 WP_002287852.1 glutamate decarboxylase -
  B4W81_RS03005 (B4W81_03005) gadC 584678..586189 (-) 1512 WP_002287851.1 glutamate:gamma-aminobutyrate antiporter -
  B4W81_RS03020 (B4W81_03020) - 587161..587646 (+) 486 Protein_564 IS256-like element ISEfm2 family transposase -
  B4W81_RS03025 (B4W81_03025) - 587842..588417 (+) 576 WP_002287847.1 thymidine kinase -
  B4W81_RS17640 - 588460..588657 (-) 198 WP_014748620.1 hypothetical protein -
  B4W81_RS03030 (B4W81_03030) - 588623..588826 (+) 204 Protein_567 IS256 family transposase -
  B4W81_RS03035 (B4W81_03035) - 588999..589391 (-) 393 WP_002287845.1 hypothetical protein -
  B4W81_RS03040 (B4W81_03040) - 589622..590701 (+) 1080 WP_077974289.1 IS30 family transposase -
  B4W81_RS03045 (B4W81_03045) - 591212..591904 (-) 693 WP_002287844.1 hypothetical protein -
  B4W81_RS03050 (B4W81_03050) - 592204..593373 (-) 1170 WP_002287843.1 IS256-like element ISEfa13 family transposase -
  B4W81_RS03055 (B4W81_03055) - 593444..593905 (-) 462 WP_225309889.1 PD-(D/E)XK nuclease family protein -
  B4W81_RS18260 - 593869..593994 (-) 126 WP_002294150.1 hypothetical protein -
  B4W81_RS03060 (B4W81_03060) rlmH 594197..594676 (-) 480 WP_002287841.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  B4W81_RS03065 (B4W81_03065) htrA 595104..596393 (+) 1290 WP_002294152.1 S1C family serine protease Regulator

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 44871.87 Da        Isoelectric Point: 4.5419

>NTDB_id=219659 B4W81_RS03065 WP_002294152.1 595104..596393(+) (htrA) [Enterococcus faecium isolate 2014-VREF-268]
MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAMGTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKA
VDKVQDAVVSVINLQSQNQSSGFGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANSVTSGIISSLNRQVTSTNESNE
TVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKIASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALG
ITMVDLSAVSTQQQEQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKVGDSVKIT
YYEGSKEKTTTVELTIDQSALKQNQNSEN

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=219659 B4W81_RS03065 WP_002294152.1 595104..596393(+) (htrA) [Enterococcus faecium isolate 2014-VREF-268]
ATGGATAGAAAAAATGTGACACCAAAGATGAAAAAAAGTAAAAATAATAGCCTCTGGCGTAAGTTAGGTCTGGGGCTTGT
TGGAGGGATCGTTGGAGGACTACTGACAGCTGGCATTTTTTATGCAGCGATGGGCACTGGCAACAATAGTTCTACTGCTA
CAAGCGGCAATCAGAATTCTGCCGGTGAAACAGTTGTGGAAAATGTCAAAGTCAATGTAGATTCTGATATCACCAAAGCG
GTAGATAAAGTTCAAGACGCTGTCGTTTCTGTTATCAACTTACAAAGTCAAAACCAATCAAGCGGTTTTGGCGGACTATT
CGGGCAGCAAGAAGAAAGTAGCAGCTCAGATGATAGTAATTTGGAAGCTTATAGTGAAGGAAGCGGCGTGATCTACAAAA
AAGACGGGAATACTGCTTATGTAGTCACGAATAATCACGTAGTAGATGGCCAGCAAGGTCTTGAAGTCTTGATGAAAGAC
GGTACGAAGGTAAAAGCTGAATTAGTTGGAACAGATGCTTATTCAGATTTAGCGGTGTTGAAGATCAATTCAGACAAAGT
CGAAACAGTTGCCTCTTTTGGTGACTCAAGTGCTCTGAAGGTTGGTGAGCCTGCGATTGCGATTGGTTCCCCACTAGGAT
CTGAATATGCAAATTCTGTCACTTCAGGAATCATTTCTTCCTTGAACCGACAAGTGACAAGTACGAATGAATCGAACGAA
ACAGTCAATATCAATGCGATCCAAACAGATGCAGCAATCAACCCTGGAAATTCTGGTGGTCCTTTAGTTAATATCGAAGG
ACAAGTTATCGGTATCAACTCAAGTAAGATCGCAAGTACTTCTGAATCTACTTCTAATGTAAGCGTAGAAGGAATGGGAT
TTGCTATTCCAAGTAACGATGTAGTGAATATCATCAATCAACTGGAAAAAGACGGAAAAGTGACACGTCCAGCTCTAGGT
ATCACAATGGTCGACTTATCTGCCGTTTCTACTCAGCAACAAGAACAAATCTTGAAGATTCCTTCATCTGTAACTAACGG
TGTCATCGTGACAAATGTCCAAACAGCTACACCGGCTGAAAAAGCTGGATTGAAACAATACGACGTCATTACTAAGATCG
ATGACAAAGAAGTTTCTTCTGGTGTAGAACTTCAATCTGCTTTGTATAAGAAAAAAGTGGGAGATTCTGTCAAAATCACT
TACTATGAAGGCTCGAAAGAAAAAACAACTACTGTTGAATTGACGATCGATCAATCGGCATTAAAACAAAATCAAAATAG
TGAAAATTAA

Domains

Predicted by InterproScan.

Peptidase family M50 (Peptidase_M50)

(9-417)

PDZ domain (PDZ_2)

(317-414)

Trypsin-like peptidase domain (Trypsin_2)

(127-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus gordonii str. Challis substr. CH1

53.269

96.27

0.513
  htrA Streptococcus mutans UA159

59.887

82.517

0.494
  htrA Streptococcus mitis NCTC 12261

52.778

92.308

0.487
  htrA Streptococcus pneumoniae TIGR4

59.05

78.555

0.464
  htrA Streptococcus pneumoniae Rx1

59.05

78.555

0.464
  htrA Streptococcus pneumoniae D39

59.05

78.555

0.464
  htrA Streptococcus pneumoniae R6

59.05

78.555

0.464

Multiple sequence alignment