Detailed information    

insolico Bioinformatically predicted

Overview

Name   htrA   Type   Regulator
Locus tag   B4W80_RS00240 Genome accession   NZ_CP019988
Coordinates   48443..49732 (-) Length   429 a.a.
NCBI ID   WP_002294152.1    Uniprot ID   A0AAV3GR07
Organism   Enterococcus faecium isolate 2014-VREF-63     
Function   repress competence development (predicted from homology)   
Competence regulation

Genomic Context

Location: 43443..54732
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4W80_RS00210 (B4W80_00210) - 43845..44792 (-) 948 WP_002289860.1 glycosyltransferase family 2 protein -
  B4W80_RS00215 (B4W80_00215) - 45015..45365 (-) 351 WP_002289862.1 PepSY domain-containing protein -
  B4W80_RS00220 (B4W80_00220) pepA 45565..46644 (+) 1080 WP_002294156.1 glutamyl aminopeptidase -
  B4W80_RS00225 (B4W80_00225) - 46788..47108 (+) 321 WP_002287837.1 thioredoxin family protein -
  B4W80_RS00230 (B4W80_00230) - 47130..47594 (+) 465 WP_002287838.1 universal stress protein -
  B4W80_RS00235 (B4W80_00235) ytpR 47799..48404 (+) 606 WP_002294153.1 YtpR family tRNA-binding protein -
  B4W80_RS00240 (B4W80_00240) htrA 48443..49732 (-) 1290 WP_002294152.1 S1C family serine protease Regulator
  B4W80_RS00245 (B4W80_00245) rlmH 50160..50639 (+) 480 WP_002287841.1 23S rRNA (pseudouridine(1915)-N(3))-methyltransferase RlmH -
  B4W80_RS17285 - 50842..50967 (+) 126 WP_002294150.1 hypothetical protein -
  B4W80_RS00250 (B4W80_00250) - 50964..51392 (+) 429 WP_229214839.1 PD-(D/E)XK nuclease family protein -
  B4W80_RS00255 (B4W80_00255) - 51463..52632 (+) 1170 WP_002287843.1 IS256-like element ISEfa13 family transposase -
  B4W80_RS00260 (B4W80_00260) - 52932..53624 (+) 693 WP_002287844.1 hypothetical protein -
  B4W80_RS00265 (B4W80_00265) - 54052..54444 (+) 393 WP_002287845.1 hypothetical protein -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 44871.87 Da        Isoelectric Point: 4.5419

>NTDB_id=219614 B4W80_RS00240 WP_002294152.1 48443..49732(-) (htrA) [Enterococcus faecium isolate 2014-VREF-63]
MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAMGTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKA
VDKVQDAVVSVINLQSQNQSSGFGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYANSVTSGIISSLNRQVTSTNESNE
TVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSKIASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALG
ITMVDLSAVSTQQQEQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKKVGDSVKIT
YYEGSKEKTTTVELTIDQSALKQNQNSEN

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=219614 B4W80_RS00240 WP_002294152.1 48443..49732(-) (htrA) [Enterococcus faecium isolate 2014-VREF-63]
ATGGATAGAAAAAATGTGACACCAAAGATGAAAAAAAGTAAAAATAATAGCCTCTGGCGTAAGTTAGGTCTGGGGCTTGT
TGGAGGGATCGTTGGAGGACTACTGACAGCTGGCATTTTTTATGCAGCGATGGGCACTGGCAACAATAGTTCTACTGCTA
CAAGCGGCAATCAGAATTCTGCCGGTGAAACAGTTGTGGAAAATGTCAAAGTCAATGTAGATTCTGATATCACCAAAGCG
GTAGATAAAGTTCAAGACGCTGTCGTTTCTGTTATCAACTTACAAAGTCAAAACCAATCAAGCGGTTTTGGCGGACTATT
CGGGCAGCAAGAAGAAAGTAGCAGCTCAGATGATAGTAATTTGGAAGCTTATAGTGAAGGAAGCGGCGTGATCTACAAAA
AAGACGGGAATACTGCTTATGTAGTCACGAATAATCACGTAGTAGATGGCCAGCAAGGTCTTGAAGTCTTGATGAAAGAC
GGTACGAAGGTAAAAGCTGAATTAGTTGGAACAGATGCTTATTCAGATTTAGCGGTGTTGAAGATCAATTCAGACAAAGT
CGAAACAGTTGCCTCTTTTGGTGACTCAAGTGCTCTGAAGGTTGGTGAGCCTGCGATTGCGATTGGTTCCCCACTAGGAT
CTGAATATGCAAATTCTGTCACTTCAGGAATCATTTCTTCCTTGAACCGACAAGTGACAAGTACGAATGAATCGAACGAA
ACAGTCAATATCAATGCGATCCAAACAGATGCAGCAATCAACCCTGGAAATTCTGGTGGTCCTTTAGTTAATATCGAAGG
ACAAGTTATCGGTATCAACTCAAGTAAGATCGCAAGTACTTCTGAATCTACTTCTAATGTAAGCGTAGAAGGAATGGGAT
TTGCTATTCCAAGTAACGATGTAGTGAATATCATCAATCAACTGGAAAAAGACGGAAAAGTGACACGTCCAGCTCTAGGT
ATCACAATGGTCGACTTATCTGCCGTTTCTACTCAGCAACAAGAACAAATCTTGAAGATTCCTTCATCTGTAACTAACGG
TGTCATCGTGACAAATGTCCAAACAGCTACACCGGCTGAAAAAGCTGGATTGAAACAATACGACGTCATTACTAAGATCG
ATGACAAAGAAGTTTCTTCTGGTGTAGAACTTCAATCTGCTTTGTATAAGAAAAAAGTGGGAGATTCTGTCAAAATCACT
TACTATGAAGGCTCGAAAGAAAAAACAACTACTGTTGAATTGACGATCGATCAATCGGCATTAAAACAAAATCAAAATAG
TGAAAATTAA

Domains

Predicted by InterproScan.

Peptidase family M50 (Peptidase_M50)

(9-417)

PDZ domain (PDZ_2)

(317-414)

Trypsin-like peptidase domain (Trypsin_2)

(127-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  htrA Streptococcus gordonii str. Challis substr. CH1

53.269

96.27

0.513
  htrA Streptococcus mutans UA159

59.887

82.517

0.494
  htrA Streptococcus mitis NCTC 12261

52.778

92.308

0.487
  htrA Streptococcus pneumoniae TIGR4

59.05

78.555

0.464
  htrA Streptococcus pneumoniae Rx1

59.05

78.555

0.464
  htrA Streptococcus pneumoniae D39

59.05

78.555

0.464
  htrA Streptococcus pneumoniae R6

59.05

78.555

0.464

Multiple sequence alignment