Detailed information    

insolico Bioinformatically predicted

Overview

Name   ciaR   Type   Regulator
Locus tag   C0J56_RS20040 Genome accession   NZ_CP025542
Coordinates   4467634..4468302 (-) Length   222 a.a.
NCBI ID   WP_134925123.1    Uniprot ID   -
Organism   Pseudomonas fluorescens strain 2P24     
Function   repress competence development; post-transcriptional repression of CSP production (predicted from homology)   
Competence regulation

Genomic Context

Location: 4462634..4473302
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C0J56_RS20025 (C0J56_20020) - 4462822..4464501 (-) 1680 WP_134925120.1 methyl-accepting chemotaxis protein -
  C0J56_RS20030 (C0J56_20025) - 4464760..4466076 (-) 1317 WP_167659391.1 Na+/H+ antiporter NhaC family protein -
  C0J56_RS20035 (C0J56_20030) - 4466324..4467637 (-) 1314 WP_134925122.1 sensor histidine kinase -
  C0J56_RS20040 (C0J56_20035) ciaR 4467634..4468302 (-) 669 WP_134925123.1 response regulator transcription factor Regulator
  C0J56_RS20045 (C0J56_20040) - 4468302..4468613 (-) 312 WP_134925124.1 PepSY domain-containing protein -
  C0J56_RS20050 (C0J56_20045) - 4468613..4468921 (-) 309 WP_134925125.1 PepSY domain-containing protein -
  C0J56_RS20055 (C0J56_20050) - 4469083..4470161 (+) 1079 Protein_3854 patatin-like phospholipase family protein -
  C0J56_RS20060 (C0J56_20055) queD 4470244..4470600 (+) 357 WP_025212792.1 6-carboxytetrahydropterin synthase QueD -
  C0J56_RS20065 (C0J56_20060) - 4470847..4471677 (+) 831 WP_134925126.1 alpha/beta hydrolase -
  C0J56_RS20070 (C0J56_20065) codB 4471957..4473228 (+) 1272 WP_134925127.1 cytosine permease -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 24794.25 Da        Isoelectric Point: 6.3369

>NTDB_id=219367 C0J56_RS20040 WP_134925123.1 4467634..4468302(-) (ciaR) [Pseudomonas fluorescens strain 2P24]
MRLLLVEDHVPLADELMAGLTRQGYAVDWLADGRDAVYQGSSEPYDLIILDLGLPGVPGLEVLAQWRAGGLATPVLILTA
RGSWAERIEGLKAGADDYLTKPFHPEELHLRVQALLRRSHGQVNQPTLKAAGLHLDEGRQCVIRDGTDIQLTAAEFRLLR
YFMLHPEQILSKSHLAEHLYDGETERDSNVLEVHVNHLRRKLGRSVIETRRGQGYLFGGQAQ

Nucleotide


Download         Length: 669 bp        

>NTDB_id=219367 C0J56_RS20040 WP_134925123.1 4467634..4468302(-) (ciaR) [Pseudomonas fluorescens strain 2P24]
ATGCGCCTGCTCCTGGTGGAAGATCACGTTCCCCTGGCCGACGAATTGATGGCCGGGCTGACACGACAGGGCTATGCCGT
CGATTGGCTGGCCGACGGTCGCGACGCGGTGTATCAAGGTAGCAGCGAGCCTTACGACCTGATCATCCTCGACCTCGGCC
TGCCGGGTGTGCCGGGGCTCGAGGTGCTGGCCCAATGGCGTGCCGGCGGCCTGGCCACACCAGTGCTGATCCTCACCGCG
CGCGGCTCCTGGGCCGAACGTATCGAAGGCCTCAAGGCCGGGGCCGACGATTACCTGACCAAACCTTTTCACCCCGAAGA
ACTGCACCTGCGGGTCCAGGCGCTGCTGCGCCGTTCCCATGGCCAGGTCAACCAGCCGACGCTCAAGGCGGCCGGGCTGC
ACTTGGATGAAGGGCGGCAATGCGTGATCCGCGACGGCACCGACATCCAGCTCACGGCCGCCGAATTTCGCCTGTTGCGA
TATTTCATGCTGCATCCCGAGCAGATCCTGTCCAAGAGCCATCTCGCTGAACACCTGTACGACGGTGAGACCGAGCGCGA
CTCCAACGTGCTGGAAGTCCATGTCAACCACCTGCGGCGCAAGCTGGGGCGCAGCGTCATTGAAACCCGTCGCGGCCAGG
GTTACCTGTTCGGCGGGCAGGCGCAGTGA

Domains

Predicted by InterProScan.

Transcriptional regulatory protein, C terminal (Trans_reg_C)

(146-217)

Response regulator receiver domain (Response_reg)

(4-112)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ciaR Streptococcus pneumoniae R6

38.462

99.55

0.383
  ciaR Streptococcus pneumoniae Rx1

38.462

99.55

0.383
  ciaR Streptococcus pneumoniae D39

38.462

99.55

0.383
  ciaR Streptococcus pneumoniae TIGR4

38.462

99.55

0.383
  ciaR Streptococcus mutans UA159

38.009

99.55

0.378
  micA Streptococcus pneumoniae Cp1015

35.897

100

0.378
  covR Lactococcus lactis subsp. lactis strain DGCC12653

35.874

100

0.36

Multiple sequence alignment