Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   Eco28_RS27570 Genome accession   NZ_CP019944
Coordinates   5379268..5380788 (+) Length   506 a.a.
NCBI ID   WP_000058987.1    Uniprot ID   A0A0K3VNE8
Organism   Escherichia coli strain DSM 103246     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 5374268..5385788
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  Eco28_RS27550 (Eco28_05319) ilvE 5375822..5376751 (-) 930 WP_021578132.1 branched-chain-amino-acid transaminase -
  Eco28_RS27555 (Eco28_05320) ilvM 5376771..5377034 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  Eco28_RS27560 (Eco28_05321) ilvG 5377031..5378677 (-) 1647 WP_001012619.1 acetolactate synthase 2 catalytic subunit -
  Eco28_RS28355 ilvX 5378680..5378730 (-) 51 WP_001387183.1 peptide IlvX -
  Eco28_RS27565 ilvL 5378817..5378915 (-) 99 WP_001311244.1 ilv operon leader peptide -
  Eco28_RS27570 (Eco28_05323) comM 5379268..5380788 (+) 1521 WP_000058987.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  Eco28_RS27575 (Eco28_05324) maoP 5380813..5381151 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  Eco28_RS27580 (Eco28_05325) hdfR 5381270..5382109 (+) 840 WP_000379246.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55261.42 Da        Isoelectric Point: 7.6190

>NTDB_id=219017 Eco28_RS27570 WP_000058987.1 5379268..5380788(+) (comM) [Escherichia coli strain DSM 103246]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=219017 Eco28_RS27570 WP_000058987.1 5379268..5380788(+) (comM) [Escherichia coli strain DSM 103246]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGTTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCCCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCCAAAAAAATTACCATCAACCTGGCTCCAGCCGATCTACCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGTGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAAGGTAAGCACACTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGTGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGTTACCTGAATTTGAACGGCGTACACTGGATGCTTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCACCACCGTTAAACAACGTGTAATGGCTGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAGTTGAACGCCTG
GCTGGATAGTCCAGAAATACGCAAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0K3VNE8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Vibrio cholerae strain A1552

59.163

99.209

0.587
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

48.193

98.419

0.474
  comM Legionella pneumophila strain ERS1305867

48.193

98.419

0.474
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443

Multiple sequence alignment