Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   BZP34_RS02750 Genome accession   NZ_CP019563
Coordinates   481117..481614 (+) Length   165 a.a.
NCBI ID   WP_029694050.1    Uniprot ID   -
Organism   Staphylococcus aureus strain SR434     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 476117..486614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BZP34_RS02710 (BZP34_02710) - 476161..477147 (+) 987 WP_000161315.1 ROK family glucokinase -
  BZP34_RS02715 (BZP34_02715) - 477147..477476 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  BZP34_RS02720 (BZP34_02720) - 477473..478096 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  BZP34_RS02725 (BZP34_02725) comGA 478148..479122 (+) 975 WP_000697219.1 competence type IV pilus ATPase ComGA Machinery gene
  BZP34_RS02730 (BZP34_02730) comGB 479094..480164 (+) 1071 WP_000776422.1 competence type IV pilus assembly protein ComGB Machinery gene
  BZP34_RS02735 (BZP34_02735) comGC 480178..480489 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  BZP34_RS02740 (BZP34_02740) comGD 480467..480913 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  BZP34_RS02745 (BZP34_02745) comGE 480900..481199 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  BZP34_RS02750 (BZP34_02750) comGF 481117..481614 (+) 498 WP_029694050.1 competence type IV pilus minor pilin ComGF Machinery gene
  BZP34_RS02755 (BZP34_02755) - 481711..481857 (+) 147 WP_001789879.1 hypothetical protein -
  BZP34_RS02760 (BZP34_02760) - 481847..482371 (+) 525 WP_001015121.1 shikimate kinase -
  BZP34_RS02765 (BZP34_02765) gcvT 482530..483621 (+) 1092 WP_000093345.1 glycine cleavage system aminomethyltransferase GcvT -
  BZP34_RS02770 (BZP34_02770) gcvPA 483641..484987 (+) 1347 WP_077156047.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  BZP34_RS02775 (BZP34_02775) gcvPB 484980..486452 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19199.92 Da        Isoelectric Point: 10.2192

>NTDB_id=216375 BZP34_RS02750 WP_029694050.1 481117..481614(+) (comGF) [Staphylococcus aureus strain SR434]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIELRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTSFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=216375 BZP34_RS02750 WP_029694050.1 481117..481614(+) (comGF) [Staphylococcus aureus strain SR434]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGAGTAGATAGAAACGATATTGAACTTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CTTCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(68-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

96.97

100

0.97
  comGF Staphylococcus aureus N315

96.97

100

0.97

Multiple sequence alignment