Detailed information NTG0215893

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BST95_RS03895
• Genome accession: NZ_CP019450
• Coordinates: 814641..815705 (+)
• Length: 354 a.a.
• NCBI ID: WP_066057213.1
• Uniprot ID: A0A162HQZ8
• Organism: Halioglobus japonicus strain NBRC 107739
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 809641..820705

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BST95_RS03880 (BST95_03905)	-	809784..810617 (+)	834	WP_169843844.1	thermonuclease family protein	-
BST95_RS03885 (BST95_03910)	-	810688..811938 (+)	1251	WP_084198238.1	malic enzyme-like NAD(P)-binding protein	-
BST95_RS03890 (BST95_03915)	-	811952..814465 (-)	2514	WP_084198239.1	penicillin-binding protein 1A	-
BST95_RS03895 (BST95_03920)	pilM	814641..815705 (+)	1065	WP_066057213.1	pilus assembly protein PilM	Machinery gene
BST95_RS03900 (BST95_03925)	-	815705..816274 (+)	570	WP_084198240.1	PilN domain-containing protein	-
BST95_RS03905 (BST95_03930)	-	816277..816894 (+)	618	WP_084198241.1	type 4a pilus biogenesis protein PilO	-
BST95_RS03910 (BST95_03935)	-	816898..817428 (+)	531	WP_084198242.1	pilus assembly protein PilP	-
BST95_RS03915 (BST95_03940)	pilQ	817699..819600 (+)	1902	WP_338073403.1	type IV pilus secretin PilQ family protein	Machinery gene
BST95_RS03920 (BST95_03945)	aroK	819600..820115 (+)	516	WP_082850170.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38037.09 Da
• Isoelectric Point: 4.1361

>NTDB_id=215893 BST95_RS03895 WP_066057213.1 814641..815705(+) (pilM) [Halioglobus japonicus strain NBRC 107739]
MLGLIGKRKATPVLGLDISSTTVKLLELSYSGNRYRVESYAVSSLPQDAVIEKNVNDVDGVANAVRNVVAQSRTKLKIAA
AAVSGSSVITKMIDMPQGLSEDDMETQLTLEADQYIPYPLEEVAIDFEVQGDSPEHPGQVEVLLAACRRETIDARVDALD
GADVTAKIVDVEAYAMERAYSLISSQLDLDGDATVAVVDIGATMTTLSVLHGGQTIYTREQLFGGKQLTDEIMRRYGLPL
EEAGLAKKQGGLPDDYEPEVLEPFKDAVVQQVARSLQFFFSSSQYNDVDYIILAGGVSSMEGLPELVQEKLGTPTQVANP
FADMSISSKVNAVALSSDAPAMMIACGLALRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=215893 BST95_RS03895 WP_066057213.1 814641..815705(+) (pilM) [Halioglobus japonicus strain NBRC 107739]
TTGCTTGGTCTAATAGGGAAAAGAAAGGCGACGCCGGTTCTGGGGCTTGATATCAGCTCGACAACGGTCAAGTTGCTTGA
GTTGAGCTACTCAGGGAATCGCTACCGTGTAGAAAGCTACGCGGTAAGTTCGCTACCGCAGGACGCGGTCATCGAGAAGA
ATGTCAATGACGTTGACGGCGTAGCCAACGCCGTGCGCAACGTTGTTGCCCAGTCCAGAACCAAACTCAAAATTGCGGCT
GCCGCCGTCTCCGGGTCGTCGGTCATCACCAAGATGATCGATATGCCCCAGGGGCTTAGTGAGGATGACATGGAGACCCA
GCTGACGCTGGAGGCCGACCAGTACATCCCCTACCCCCTGGAAGAGGTGGCGATCGACTTCGAGGTGCAGGGCGATTCGC
CCGAGCACCCCGGTCAGGTGGAAGTCTTGCTGGCGGCCTGTCGTCGTGAGACGATCGATGCCAGGGTAGACGCCCTTGAC
GGCGCGGACGTCACCGCCAAAATTGTCGATGTGGAAGCCTATGCGATGGAGCGGGCTTACTCGTTAATCAGCAGCCAGCT
CGATCTGGATGGCGACGCCACGGTGGCGGTGGTCGATATCGGTGCCACCATGACCACGCTCAGTGTCTTGCACGGTGGCC
AGACCATCTACACCCGCGAACAGCTTTTTGGCGGCAAGCAGCTGACTGATGAAATCATGCGCCGCTACGGCCTGCCCCTT
GAAGAAGCGGGTCTTGCCAAGAAGCAGGGCGGTTTGCCCGACGATTACGAGCCTGAAGTGCTCGAGCCTTTCAAAGACGC
GGTCGTTCAGCAGGTGGCCCGATCCCTGCAGTTTTTCTTCTCGTCCAGTCAGTACAACGACGTGGACTACATTATCCTCG
CCGGCGGGGTCTCCTCCATGGAGGGGCTGCCCGAATTGGTCCAGGAAAAACTCGGCACGCCCACCCAGGTGGCGAATCCT
TTCGCTGACATGTCAATTTCGTCCAAGGTAAACGCCGTGGCCCTGAGCAGCGATGCGCCGGCCATGATGATTGCCTGCGG
CCTTGCCCTGCGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (15-353)

3D structure

• AlphaFold DB: A0A162HQZ8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	53.216	96.61	0.514
comM	Acinetobacter nosocomialis M2	53.216	96.61	0.514
comM	Acinetobacter baylyi ADP1	50	98.305	0.492
pilM	Legionella pneumophila strain ERS1305867	46.479	100	0.466