Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PspR84_RS02290 Genome accession   NZ_CP019426
Coordinates   499407..500471 (+) Length   354 a.a.
NCBI ID   WP_160055101.1    Uniprot ID   A0A7Z2KPH3
Organism   Pseudomonas sp. R84     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 494407..505471
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR84_RS02280 (PspR84_02310) - 495154..496422 (+) 1269 WP_038360054.1 malic enzyme-like NAD(P)-binding protein -
  PspR84_RS02285 (PspR84_02315) - 496743..499190 (-) 2448 WP_160055098.1 penicillin-binding protein 1A -
  PspR84_RS02290 (PspR84_02320) pilM 499407..500471 (+) 1065 WP_160055101.1 pilus assembly protein PilM Machinery gene
  PspR84_RS02295 (PspR84_02325) - 500471..501037 (+) 567 WP_160055104.1 PilN domain-containing protein -
  PspR84_RS02300 (PspR84_02330) pilO 501034..501657 (+) 624 WP_160055107.1 type 4a pilus biogenesis protein PilO -
  PspR84_RS02305 (PspR84_02335) - 501654..502181 (+) 528 WP_160055109.1 pilus assembly protein PilP -
  PspR84_RS02310 (PspR84_02340) pilQ 502195..504276 (+) 2082 WP_160055111.1 type IV pilus secretin PilQ Machinery gene
  PspR84_RS02315 (PspR84_02345) aroK 504281..504799 (+) 519 WP_011332081.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38154.74 Da        Isoelectric Point: 4.8109

>NTDB_id=215632 PspR84_RS02290 WP_160055101.1 499407..500471(+) (pilM) [Pseudomonas sp. R84]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPVNAVVEKNIAELEGVGQALSRVLVKARTGLKSVAV
AVAGSAVITKVIEMDAGLTDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSPRNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAATQERLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTL
EQAGLAKKQGGLPDDYISEVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVSGLDRLIEQRLNTPTQVANP
FANMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=215632 PspR84_RS02290 WP_160055101.1 499407..500471(+) (pilM) [Pseudomonas sp. R84]
GTGCTGGGACTCTTCAATAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCTACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTACGCGGTAGAGCCATTGCCGGTCAACGCCGTGGTCGAAAAGAACA
TCGCCGAGCTTGAAGGCGTCGGCCAGGCCCTGAGCCGGGTGCTGGTCAAGGCGCGCACCGGGCTCAAAAGCGTGGCGGTC
GCGGTGGCCGGTTCCGCCGTGATCACCAAAGTCATCGAAATGGACGCCGGGCTGACCGACGACGAACTGGAAAACCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCGCTGGACGAAGTCGCCATCGATTTCGAAGTCCAGGGCGTTTCGCCGC
GCAACCCTGAACGAGTCAACGTGCTGCTGGCAGCTTGTCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCACTC
GCCGGGCTGACTGCGCGGGTCGTTGATGTCGAAGCCTATGCGCTGGAGCGCTCTTTCGGGTTGCTCGCCACGCAACTGGC
GGCCACGCAAGAGCGGCTCACTGTCGCCGTCGTCGATATCGGCGCGACCATGACCACCCTTAGCGTCTTGCACAACGGCA
AGATCATCTACACCCGCGAACAATTGTTTGGCGGTCGGCAATTGACCGAGGAGATCCAGCGGCGTTATGGCCTCACCCTC
GAGCAGGCGGGGCTGGCGAAAAAACAGGGCGGCCTGCCCGATGATTACATCAGCGAGGTCTTGCAGCCGTTTCGAGAGGC
GCTGGTCCAGCAGGTTTCGCGCTCGCTGCAGTTCTTCTTCGCTTCCGGGCAATACAACGCGGTCGACCACATTCTGCTGG
CTGGCGGCACGGCGTCGGTATCCGGGCTGGATCGGCTGATCGAGCAGCGCCTGAACACGCCAACGCAAGTGGCCAACCCG
TTTGCCAACATGGCCTTGAGCAGCAAGGTCAACGCCGGGGCGCTGGCCAGTGACGCGCCGGCCCTGATGATCGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2KPH3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

47.175

100

0.472

Multiple sequence alignment