Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PspR84_RS02310 Genome accession   NZ_CP019426
Coordinates   502195..504276 (+) Length   693 a.a.
NCBI ID   WP_160055111.1    Uniprot ID   A0A7Z2QLP0
Organism   Pseudomonas sp. R84     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 497195..509276
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR84_RS02290 (PspR84_02320) pilM 499407..500471 (+) 1065 WP_160055101.1 pilus assembly protein PilM Machinery gene
  PspR84_RS02295 (PspR84_02325) - 500471..501037 (+) 567 WP_160055104.1 PilN domain-containing protein -
  PspR84_RS02300 (PspR84_02330) pilO 501034..501657 (+) 624 WP_160055107.1 type 4a pilus biogenesis protein PilO -
  PspR84_RS02305 (PspR84_02335) - 501654..502181 (+) 528 WP_160055109.1 pilus assembly protein PilP -
  PspR84_RS02310 (PspR84_02340) pilQ 502195..504276 (+) 2082 WP_160055111.1 type IV pilus secretin PilQ Machinery gene
  PspR84_RS02315 (PspR84_02345) aroK 504281..504799 (+) 519 WP_011332081.1 shikimate kinase AroK -
  PspR84_RS02320 (PspR84_02350) aroB 504952..506052 (+) 1101 WP_160055114.1 3-dehydroquinate synthase -
  PspR84_RS02325 (PspR84_02355) - 506063..507655 (+) 1593 WP_160055117.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75133.66 Da        Isoelectric Point: 5.8278

>NTDB_id=215633 PspR84_RS02310 WP_160055111.1 502195..504276(+) (pilQ) [Pseudomonas sp. R84]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTESPARIALDLPGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVSLTQLAPYNTRVEGNNLFVVVGQGAPAAAPRPAAVAPRATAKAVAPAKAFVPKNRAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDKLRVRLDVKDFATPVQFVNAGVTADRTVITVEPSG
TYEYSTFQTDNKLTVSVRPMTVDDLQKRNADRQAYVGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDMGTVGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=215633 PspR84_RS02310 WP_160055111.1 502195..504276(+) (pilQ) [Pseudomonas sp. R84]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGATGTGGCAGCGTTGCCGGGTGATCGCGTCGAGCTGAAGTTGTCGTTCGACGGCCCGCCGCCGCAACCCAAGG
GCTACACCACTGAATCGCCGGCGCGGATCGCCCTGGATCTGCCCGGTGTCGCCAGTCAGTTGGCCAGCAAGAATCTTGAT
CTGGGCAGTGGCAATGCGCGCACGGCCACGGTGGTCGAGGCCAAGGATCGGACTCGGTTGATCGTCAGCCTCACGCAACT
GGCGCCTTACAACACGCGGGTCGAAGGCAATAACCTGTTCGTGGTGGTCGGTCAGGGCGCACCGGCAGCGGCGCCGCGTC
CTGCTGCCGTTGCACCTCGTGCTACTGCTAAAGCGGTTGCGCCCGCCAAGGCCTTTGTGCCGAAAAACCGGGCGATTCGC
GGTGTGGACTTCCAGCGCGGTACGGCAGGTGAAGGCAATGTCGTCATCGACCTGTCCGACCCGACCATTGCCCCGGATAT
CCAGGAGCATGACGGCAAGATCATCCTCAGCTTTGCCCGCACGCAGTTGCCGGACAAGCTGCGGGTGCGCCTTGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCGGAGTGACCGCTGATCGCACGGTTATTACCGTTGAACCCAGTGGT
ACTTACGAGTACTCGACCTTCCAGACCGACAACAAACTGACCGTCAGCGTCCGCCCTATGACGGTCGATGACCTGCAAAA
GCGCAACGCCGATCGTCAGGCTTACGTCGGCGAAAAGCTTTCGCTGAATTTCCAGGACATTGATGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAACCTTGTCGCCAGCGACACGGTGCAGGGTGGCATCACTTTGCGCCTGCAAAAC
GTGCCGTGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAAGGTCTGGATAAACGCAAGATCGGCAACGTGCTGCTGGT
GGCTCCCGCCGATGAAATCGCTGCCCGCGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGAGCTGGCGCCGTTGC
GCCGTGAACTGCTGCAAGTGAACTACGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCCGAGGCT
AAAATCGATGAGCGCGGTTCGATTACCGTCGATGAGCGAACCAACAACATCATTGCCTACCAGACTCAGGATCGTCTCGA
CGAACTGCGGCGGATCGTGGCGCAGCTGGATATTCCGGTGCGTCAGGTGATGATCGAGGCGCGGATCGTCGAGGCTAACG
TCGATTACGACAAAAGCCTGGGCGTACGCTGGGGCGGGTCGATCCAGAACAAGGGTAACTGGAACACTTCGGGCGTCAGT
AATGGCTCATCGACCACCATCGGCACACCAGGCAGCACCAGCACCAACTCGCCGTTCGTCGACATGGGGACCGTGGGTAA
TACCTCGGGGATCGGCATTGCCTTCATCACTGACAATGTCTTGCTCGATCTCGAGCTGACAGCGATGGAGAAAACCGGCA
ACGGTGAAATCGTCTCGCAGCCGAAAGTGGTCACCTCCGACAAGGAAACCGCAAAAATCCTCAAAGGCACCGAGATTCCT
TATCAGGAAGCCAGCTCAAGCGGCGCGACGTCGGTGTCGTTCAAGGAGGCCTCGCTGTCACTGGAAGTCACCCCGCAGAT
CACCCCCGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGATTACCTGAACAAAGTGCAGGATGTGC
CGCCGATCAAGAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGACGGCGAAACCATCGTGATTGGCGGTGTTTTCTCA
AATACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACACCACGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-122)

Bacterial type II and III secretion system protein (Secretin)

(528-684)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Z2QLP0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.324

100

0.758
  comQ Acinetobacter baylyi ADP1

41.21

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.005

100

0.407
  pilQ Acinetobacter baumannii D1279779

38.005

100

0.407
  pilQ Legionella pneumophila strain Lp02

38.166

97.547

0.372
  pilQ Legionella pneumophila strain ERS1305867

38.166

97.547

0.372

Multiple sequence alignment