Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   BWI85_RS08385 Genome accession   NZ_CP019256
Coordinates   1601438..1602958 (+) Length   506 a.a.
NCBI ID   WP_000058989.1    Uniprot ID   A7ZTW9
Organism   Escherichia coli strain 13TMH22     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 1596438..1607958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI85_RS08355 (BWI85_08505) ilvE 1597992..1598921 (-) 930 WP_000208520.1 branched-chain-amino-acid transaminase -
  BWI85_RS08360 (BWI85_08510) ilvM 1598941..1599204 (-) 264 WP_000983255.1 acetolactate synthase 2 small subunit -
  BWI85_RS08365 (BWI85_08515) ilvG 1599201..1600847 (-) 1647 WP_001012633.1 acetolactate synthase 2 catalytic subunit -
  BWI85_RS08370 ilvX 1600850..1600900 (-) 51 WP_001387183.1 peptide IlvX -
  BWI85_RS08375 (BWI85_08520) ilvL 1600987..1601085 (-) 99 WP_001311244.1 ilv operon leader peptide -
  BWI85_RS08385 (BWI85_08525) comM 1601438..1602958 (+) 1521 WP_000058989.1 YifB family Mg chelatase-like AAA ATPase Machinery gene
  BWI85_RS08390 (BWI85_08530) maoP 1602984..1603322 (-) 339 WP_000841001.1 macrodomain Ori organization protein MaoP -
  BWI85_RS08395 (BWI85_08535) hdfR 1603441..1604280 (+) 840 WP_000379246.1 HTH-type transcriptional regulator HdfR -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55261.38 Da        Isoelectric Point: 7.3875

>NTDB_id=214435 BWI85_RS08385 WP_000058989.1 1601438..1602958(+) (comM) [Escherichia coli strain 13TMH22]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
QAVCAFLEGKHTLERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=214435 BWI85_RS08385 WP_000058989.1 1601438..1602958(+) (comM) [Escherichia coli strain 13TMH22]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTCTACCCGGTTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCCCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCCAAAAAAATCACCATCAACCTGGCTCCAGCCGATCTGCCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGTGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGATAGCCGATCATCTG
CAGGCTGTCTGTGCGTTTCTGGAAGGTAAGCACACTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGTGGGCACAACCTTT
TACTGATTGGACCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGCCTGTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACTGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGCTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGTTACCTGAGTTTGAACGGCGTACACTGGATGCCTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTCGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCACCACCGTTAAACAACGTGTAATGGCTGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAGTTGAACGCCTG
GCTGGATAGTCCGGAAATACGCCAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCCGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCCATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A7ZTW9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Vibrio cholerae strain A1552

59.363

99.209

0.589
  comM Glaesserella parasuis strain SC1401

58.58

100

0.587
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

48.193

98.419

0.474
  comM Legionella pneumophila strain ERS1305867

48.193

98.419

0.474
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443

Multiple sequence alignment