Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BI314_RS07550 Genome accession   NZ_CP018847
Coordinates   1681497..1683233 (+) Length   578 a.a.
NCBI ID   WP_011052125.1    Uniprot ID   A0AAI7ZH88
Organism   Xanthomonas citri pv. citri strain LL074-4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1684075..1707557 1681497..1683233 flank 842


Gene organization within MGE regions


Location: 1681497..1707557
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BI314_RS07550 (BI314_07550) pilB 1681497..1683233 (+) 1737 WP_011052125.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BI314_RS07560 (BI314_07560) pilB 1683727..1683846 (+) 120 WP_005915819.1 hypothetical protein Machinery gene
  BI314_RS07565 (BI314_07565) - 1683978..1684145 (+) 168 WP_015463523.1 hypothetical protein -
  BI314_RS07570 (BI314_07570) pilR 1684368..1685762 (-) 1395 WP_015463522.1 sigma-54 dependent transcriptional regulator Regulator
  BI314_RS07575 (BI314_07575) - 1686087..1687700 (-) 1614 WP_003488599.1 HAMP domain-containing sensor histidine kinase -
  BI314_RS07580 (BI314_07580) sucC 1687932..1689101 (+) 1170 WP_005915812.1 ADP-forming succinate--CoA ligase subunit beta -
  BI314_RS07585 (BI314_07585) sucD 1689126..1690001 (+) 876 WP_005921370.1 succinate--CoA ligase subunit alpha -
  BI314_RS07590 (BI314_07590) - 1690088..1690603 (+) 516 WP_011052123.1 hypothetical protein -
  BI314_RS07595 (BI314_07595) - 1690558..1691673 (-) 1116 WP_011052122.1 IS1595 family transposase -
  BI314_RS07600 (BI314_07600) - 1691901..1692185 (-) 285 WP_016849322.1 hypothetical protein -
  BI314_RS07610 (BI314_07610) xopAI 1693516..1694406 (-) 891 WP_011052119.1 type III secretion system effector XopAI -
  BI314_RS07615 (BI314_07615) - 1694540..1695621 (-) 1082 Protein_1391 DNA-binding protein -
  BI314_RS07620 (BI314_07620) xopE 1695952..1697028 (-) 1077 WP_041470856.1 XopE/AvrPphe family type III secretion system effector -
  BI314_RS07625 (BI314_07625) - 1697161..1698216 (-) 1056 WP_011052969.1 DNA-binding protein -
  BI314_RS07630 (BI314_07630) - 1698408..1699682 (+) 1275 WP_011052968.1 tyrosine-type recombinase/integrase -
  BI314_RS07635 (BI314_07635) - 1699690..1702679 (+) 2990 Protein_1395 Tn3-like element TnXax1 family transposase -
  BI314_RS07640 (BI314_07640) - 1702812..1704089 (+) 1278 WP_011052115.1 lytic murein transglycosylase -
  BI314_RS07645 (BI314_07645) avrXacE2 1704175..1705245 (+) 1071 WP_011052114.1 type III secretion system effector avirulence protein AvrXacE2 -
  BI314_RS07655 (BI314_07655) - 1706005..1707172 (-) 1168 Protein_1398 IS3 family transposase -
  BI314_RS07665 (BI314_07665) - 1707210..1707557 (+) 348 WP_040107789.1 endonuclease domain-containing protein -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62517.63 Da        Isoelectric Point: 5.4526

>NTDB_id=210995 BI314_RS07550 WP_011052125.1 1681497..1683233(+) (pilB) [Xanthomonas citri pv. citri strain LL074-4]
MNSVVTANLVGITGIARRLVQDGAVEEAVARSAMDQASAAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLLDVSAFDAS
QNAVKLVSEELLQKHQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSALG
DDEEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQK
LFLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
DNALLAEGFTPAQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQAIGIRDLRQSALM
KAANGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=210995 BI314_RS07550 WP_011052125.1 1681497..1683233(+) (pilB) [Xanthomonas citri pv. citri strain LL074-4]
ATGAATAGCGTCGTGACCGCAAATTTGGTTGGAATAACGGGCATCGCGCGTCGTCTGGTGCAAGACGGTGCGGTCGAAGA
AGCAGTTGCGCGATCTGCGATGGATCAGGCATCTGCGGCAAAGGTTCCTCTGCCGCAATGGTTTGCTGAGAAGAAGTTGG
TCACTGCTTCCCAGCTCGCCGCTGCGAATGCGGTCGAGTTCGGCATGCCACTGTTGGACGTGTCGGCGTTCGACGCCAGC
CAAAACGCGGTCAAGCTGGTAAGTGAGGAGCTACTTCAGAAGCACCAAGTGCTACCGCTGTTCAAGCGCGGCAACCGGCT
GTTCGTAGGGGTGAGCAATCCGACCCAGACCCGGGCGCTGGATGACATCAAGTTTCATACGAACTTGGTAGTCGAGCCCA
TCCTTGTGGATGAAGATCAGATCCGTCGCACCCTGGAGCAATGGCAGGCCAGCAATGCTGCGCTGGGCTCCGCGCTCGGT
GACGACGAGGAGGGCATGGGGGATCTGGACGTCTCGGCCGGCGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTTGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCAGGCGGGGAGCCTCGGACA
TCCATTTCGAGCCGTATGAAGACGACTACCGGGTGCGCTTGCGCATCGATGGCTTGTTGAAGAACGTGGCCAAGGCGCCG
GTGAAGCTGAACCAGCGCATCGCAGCGCGGTTGAAGGTGATGTCGCAGCTGGATATCGCCGAGAAGCGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCCTGTTCGGCGAGA
AGGTGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGCTACGAGCCGGACCAACAGAAG
CTGTTCCTGGAAGCGATCCACAAGCCCTATGGCATGGTGCTGGTGACCGGGCCGACCGGCTCGGGCAAGACGGTGTCGTT
GTACACCGCGCTGGGAATTCTCAACGACGAGACGCGCAATATCTCCACCGCGGAGGATCCGGTCGAAATCCGCTTGCCTG
GCGTCAATCAGGTGCAGCAGAACAACAAGCGTGGCATGACCTTCGCCGCGGCTTTGCGCTCGTTCCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGTGACCTGGAGACGGCCGAGATTGCGATCAAGGCGGCGCAGACGGGTCACATGGT
GCTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATCGCACGTCTGATGAACATGGGCATCGCGCCCTACAACATCA
CCTCGTCGGTGACGCTGGTGATCGCGCAGCGGCTGGCGCGGCGCTTGTGCAACAACTGCAAGCGCAAGTCGACGCTGCCT
GACAACGCATTGCTGGCCGAAGGATTCACGCCTGCCCAGCTTGCCGCCGGGATCGAGCTGTATGAGGCGGTCGGTTGCGA
TGAGTGCACCGAAGGCTACAAGGGGCGTACCGGTATCTACCAGGTAATGCCGATGACCGACGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCGATGCAGATCGCCGAGGCCGCGCAGGCGATCGGTATCCGCGATTTGCGGCAGTCGGCGCTGATG
AAGGCTGCGAATGGGGTGACCAGCCTGGCCGAGATCAATCGTGTGACGAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.514

98.27

0.555
  pilB Acinetobacter baylyi ADP1

55.81

98.27

0.548
  pilB Legionella pneumophila strain ERS1305867

52.021

98.443

0.512
  pilB Vibrio cholerae strain A1552

49.306

99.654

0.491
  pilF Neisseria gonorrhoeae MS11

49.12

98.27

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

46.964

96.886

0.455
  pilB Vibrio campbellii strain DS40M4

45.518

98.443

0.448

Multiple sequence alignment