Detailed information NTG0209959

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: BTU49_RS14300
• Genome accession: NZ_CP018756
• Coordinates: 3133139..3134869 (-)
• Length: 576 a.a.
• NCBI ID: WP_079222942.1
• Uniprot ID: -
• Organism: Stenotrophomonas maltophilia strain AA1
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	3122266..3144076	3133139..3134869	within	0

Gene organization within MGE regions

Location: 3122266..3144076

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BTU49_RS14255	-	3122266..3122940 (+)	675	WP_079222934.1	hypothetical protein	-
BTU49_RS14260	-	3122934..3123947 (+)	1014	WP_079222935.1	hypothetical protein	-
BTU49_RS14265	-	3123941..3124957 (+)	1017	WP_079222936.1	TraB/GumN family protein	-
BTU49_RS14275	-	3125621..3127354 (+)	1734	WP_152027108.1	ABC transporter transmembrane domain-containing protein	-
BTU49_RS14280	sucD	3127473..3128348 (-)	876	WP_005410793.1	succinate--CoA ligase subunit alpha	-
BTU49_RS14285	sucC	3128370..3129539 (-)	1170	WP_079222939.1	ADP-forming succinate--CoA ligase subunit beta	-
BTU49_RS14290	-	3129974..3131587 (+)	1614	WP_079222940.1	ATP-binding protein	-
BTU49_RS14295	pilR	3131643..3133016 (+)	1374	WP_079222941.1	sigma-54 dependent transcriptional regulator	Regulator
BTU49_RS14300	pilB	3133139..3134869 (-)	1731	WP_079222942.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BTU49_RS14305	-	3135144..3136019 (+)	876	WP_079222943.1	glycosyltransferase family 2 protein	-
BTU49_RS14310	-	3136016..3137179 (+)	1164	WP_079222944.1	glycosyltransferase family 4 protein	-
BTU49_RS14315	-	3137330..3138580 (+)	1251	WP_108747253.1	oligosaccharide flippase family protein	-
BTU49_RS14320	-	3138543..3139658 (-)	1116	WP_079222946.1	glycosyltransferase	-
BTU49_RS14325	-	3139655..3140515 (-)	861	WP_161490384.1	FkbM family methyltransferase	-
BTU49_RS14330	-	3140624..3141739 (+)	1116	WP_079222948.1	glycosyltransferase	-
BTU49_RS14335	-	3141725..3142141 (-)	417	WP_079222949.1	pilin	-
BTU49_RS14340	-	3142145..3144076 (-)	1932	WP_079222950.1	hypothetical protein	-

Sequence

Protein

• Length: 576 a.a.
• Molecular weight: 62440.53 Da
• Isoelectric Point: 6.0915

>NTDB_id=209959 BTU49_RS14300 WP_079222942.1 3133139..3134869(-) (pilB) [Stenotrophomonas maltophilia strain AA1]
MNAVTTANLVGITGLARRLVQDGALDEAAARDAMAKATAARLPLPTWFAQNKVVGAAQLAAANSVEFGMPLFDVSTFDSS
QNAIALVSEELLRKHNVLPLFKRGGRLFVGTSNPTHSLDEIKFHTNLVVEPILIDEDQIRRTLEQWQAKHDTMSDALGGD
DEGMANLDVSGGDEDLAGSSDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPEQQKLF
LEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCSNCKRPSTLPAH
ALLAEGFTQEQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEISTIILAGGNALQIAEAAQAIKVNDLRQSALKKA
AAGVTSLAEINRVTKD

Nucleotide

• Length: 1731 bp

>NTDB_id=209959 BTU49_RS14300 WP_079222942.1 3133139..3134869(-) (pilB) [Stenotrophomonas maltophilia strain AA1]
ATGAACGCCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCGCGCCGCCTGGTCCAGGATGGCGCGCTGGATGA
AGCTGCTGCCCGCGATGCGATGGCCAAGGCTACCGCCGCACGCCTGCCCCTCCCGACCTGGTTCGCGCAGAACAAAGTCG
TGGGCGCCGCGCAGCTGGCCGCCGCCAATTCGGTCGAATTCGGCATGCCGCTGTTCGATGTGTCCACGTTCGACAGCAGC
CAGAACGCCATTGCGCTGGTCAGCGAGGAGCTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGTGGCCGCCT
GTTCGTCGGCACCAGCAACCCGACCCATTCGCTGGATGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTCA
TCGACGAGGACCAGATCCGCCGCACGCTGGAGCAGTGGCAGGCCAAGCATGACACCATGTCCGATGCGCTCGGTGGCGAT
GACGAGGGCATGGCCAACCTCGACGTCAGCGGTGGCGACGAGGATCTCGCTGGCAGCAGCGACAGTGGCATCGACGCCAA
GGGTGACGATACCCCGGTGGTCAAGTTCGTCAACAAGGTGCTGGTCGATGCGATCCGCAAGGGCGCTTCGGACATCCACT
TCGAACCCTATGAAGACGACTACCGCGTGCGCCTGCGCATCGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
TTGAACCAGCGCATCGCCGCGCGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTGAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTCAGCACGCTGCCGACCCTGTTCGGCGAAAAGGTGG
TGCTGCGTATCCTGGATGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGTTACGAGCCGGAGCAGCAGAAGCTGTTC
CTGGAGGCGATCCACAAGCCGTACGGCATGGTCCTGGTGACCGGCCCGACTGGTTCGGGCAAGACGGTGTCGCTGTATAC
CGCGCTGGGCATTCTCAACGACGAGACCCGCAACATCTCCACCGCCGAAGATCCGGTGGAAATCCGCCTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTTGCCGCCGCGCTGCGCTCGTTCCTGCGACAGGATCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGCCACATGGTGCTGTC
CACCCTGCATACCAACGACGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATCGCGCCGTACAACATCACCAGTT
CGGTCACCCTGGTGATCGCCCAGCGCCTGGCGCGCCGCCTGTGCAGCAACTGCAAGCGTCCGTCCACCCTGCCGGCGCAC
GCGCTGCTGGCCGAGGGCTTCACCCAGGAGCAGCTGGATGCGGGCATCGAACTGTACGAAGCGGTGGGCTGCGATGAGTG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCTCGACGATCATCCTGGCCG
GCGGCAATGCCTTGCAGATCGCAGAGGCAGCGCAGGCGATCAAGGTGAACGACCTGCGCCAGTCCGCGCTGAAGAAGGCC
GCGGCGGGCGTGACCAGCCTGGCCGAGATCAACCGCGTCACCAAGGATTGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (49-150)

• Type II/IV secretion system protein (T2SSE) (199-466)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baylyi ADP1	55.613	100	0.559
pilB	Acinetobacter baumannii D1279779	55.282	98.611	0.545
pilB	Legionella pneumophila strain ERS1305867	51.22	99.653	0.51
pilB	Vibrio cholerae strain A1552	48.687	99.132	0.483
pilF	Neisseria gonorrhoeae MS11	48.858	98.785	0.483
pilB	Vibrio parahaemolyticus RIMD 2210633	46.208	98.438	0.455
pilB	Vibrio campbellii strain DS40M4	44.852	99.479	0.446