Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   ECs_4700 Genome accession   NC_002695
Coordinates   4742019..4743539 (-) Length   506 a.a.
NCBI ID   NP_312727.2    Uniprot ID   Q8XAV5
Organism   Escherichia coli O157:H7 str. Sakai     
Function   require for natural transformation (predicted from homology)   
Unclear

Genomic Context

Location: 4737019..4748539
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECs_4698 hdfR 4740698..4741537 (-) 840 NP_312725.1 flhDC operon transcriptional repressor -
  ECs_4699 yifE 4741656..4741994 (+) 339 NP_312726.1 hypothetical protein -
  ECs_4700 comM 4742019..4743539 (-) 1521 NP_312727.2 magnesium chelatase family protein and transcriptional regulator Machinery gene
  ECs_4701 - 4743892..4743990 (+) 99 NP_312728.1 livGEDA operon leader peptide -
  ECs_4702 ilvG 4744130..4745776 (+) 1647 NP_312729.1 acetolactate synthase 2 large subunit -
  ECs_4703 ilvM 4745773..4746036 (+) 264 NP_312730.1 acetolactate synthase 2 small subunit -
  ECs_4704 ilvE 4746056..4746985 (+) 930 NP_312731.1 branched-chain amino acid aminotransferase -

Sequence

Protein


Download         Length: 506 a.a.        Molecular weight: 55217.37 Da        Isoelectric Point: 7.6190

>NTDB_id=20839 ECs_4700 NP_312727.2 4742019..4743539(-) (comM) [Escherichia coli O157:H7 str. Sakai]
MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKEARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGR
YDLPIAIALLAASEQLTANKLDEYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLVADHL
QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGPPGTGKTMLASRINGLLPDLSNE
EALESAAILSLVNAESVQKQWRQRPFRSPHHSASLTAMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIES
GQIHLSRTRAKITYPARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGILSKTVVPG
ESSTTVKQRVMAARERQFKRQNKLNAWLDSPEIRKFCKLESEDAQWLEETLIHLGLSIRAWQRLLKVARTIADIDQSDII
TRQHLQEAVSYRAIDRLLIHLQKLLT

Nucleotide


Download         Length: 1521 bp        

>NTDB_id=20839 ECs_4700 NP_312727.2 4742019..4743539(-) (comM) [Escherichia coli O157:H7 str. Sakai]
ATGTCACTGTCAATTGTTCATACCCGCGCAGCCCTGGGAGTAAATGCGCCCCCAATCACTGTTGAGGTACATATCAGTAA
AGGTTTGCCCGGCTTAACGATGGTGGGCTTACCAGAAACAACGGTAAAAGAAGCTCGCGATCGCGTGCGCAGCGCCATTA
TCAATAGCGGATATGAATATCCGGCGAAAAAAATCACCATTAACCTTGCTCCAGCCGATCTACCAAAAGAAGGGGGACGA
TATGATTTACCTATCGCCATTGCGTTGCTGGCGGCCTCAGAACAGCTTACAGCCAATAAGTTAGATGAATATGAATTAGT
CGGAGAACTGGCGCTTACAGGCGCTCTGCGTGGCGTTCCCGGTGCAATCTCCAGTGCAACTGAAGCTATTAAGTCGGGCA
GAAAAATTATCGTCGCGAAAGATAACGAAGATGAAGTGGGGCTAATTAACGGTGAAGGATGCCTGGTAGCCGATCATCTG
CAAGCTGTCTGTGCGTTTCTGGAAGGTAAGCACGCTCTCGAACGCCCGAAACCAACTGATGCAGTATCCCGGGCGCTACA
ACATGATCTCAGTGATGTTGTCGGTCAGGAGCAAGGAAAGCGAGGACTGGAAATTACCGCCGCTGGCGGGCACAACCTTT
TACTGATTGGGCCGCCGGGAACAGGTAAAACAATGCTCGCCAGCCGTATTAATGGTCTTTTGCCAGATTTAAGCAATGAA
GAGGCACTGGAGAGCGCTGCGATATTAAGTCTGGTAAATGCTGAATCAGTACAAAAACAATGGCGGCAGCGCCCGTTCCG
CTCACCTCATCACAGTGCGTCGTTAACGGCGATGGTGGGCGGTGGTGCAATTCCAGGGCCAGGTGAAATTTCGTTGGCGC
ATAACGGCGTGCTTTTTCTTGATGAGCTACCTGAATTTGAACGGCGTACACTGGATGCTTTGCGAGAGCCGATTGAATCC
GGGCAGATCCATCTTTCACGCACACGAGCAAAAATAACCTATCCAGCCCGTTTCCAGCTTGTTGCGGCGATGAATCCCAG
CCCTACCGGACATTATCAGGGAAACCATAACCGCTGCACGCCAGAACAGACATTACGTTATCTCAACCGGCTCTCGGGGC
CCTTTCTCGACCGCTTCGATCTCTCACTGGAGATCCCATTACCACCCCCCGGCATTTTGAGTAAAACGGTAGTGCCGGGA
GAAAGCAGCACCACCGTTAAACAACGTGTAATGGCTGCCAGAGAGCGCCAATTTAAGCGGCAGAATAAGTTGAACGCCTG
GCTGGATAGTCCGGAAATACGCAAATTCTGCAAGCTTGAGAGCGAAGATGCGCAGTGGCTGGAAGAAACGCTGATCCATC
TGGGGTTATCGATTCGTGCCTGGCAGCGGTTATTGAAAGTTGCACGAACCATTGCTGATATTGATCAGTCTGACATTATC
ACACGTCAGCATTTGCAGGAGGCAGTTAGCTATCGTGCGATTGACCGTTTGCTCATCCATCTGCAAAAACTACTGACATA
A

Domains

Predicted by InterproScan.

Subunit ChlI of Mg-chelatase (ChlI)

(21-142)

Magnesium chelatase, subunit ChlI C-terminal (Mg_chelatase_C)

(400-492)

Magnesium chelatase, subunit ChlI (Mg_chelatase)

(189-386)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8XAV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Haemophilus influenzae Rd KW20

58.513

100

0.591
  comM Glaesserella parasuis strain SC1401

58.383

100

0.585
  comM Vibrio cholerae strain A1552

58.964

99.209

0.585
  comM Vibrio campbellii strain DS40M4

57.968

99.209

0.575
  comM Legionella pneumophila str. Paris

48.193

98.419

0.474
  comM Legionella pneumophila strain ERS1305867

48.193

98.419

0.474
  RA0C_RS07335 Riemerella anatipestifer ATCC 11845 = DSM 15868

44.269

100

0.443

Multiple sequence alignment