Detailed information NTG0208135

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BS103_RS16180
• Genome accession: NZ_CP018332
• Coordinates: 3359204..3360262 (-)
• Length: 352 a.a.
• NCBI ID: WP_002014066.1
• Uniprot ID: N9LI98
• Organism: Acinetobacter baumannii strain A1296
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3354204..3365262

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BS103_RS16155 (BS103_16140)	aroK	3354479..3355048 (-)	570	WP_000643712.1	shikimate kinase AroK	-
BS103_RS16160 (BS103_16145)	pilQ	3355060..3357225 (-)	2166	WP_002062175.1	type IV pilus secretin PilQ family protein	Machinery gene
BS103_RS16165 (BS103_16150)	pilP	3357288..3357815 (-)	528	WP_000695065.1	pilus assembly protein PilP	Machinery gene
BS103_RS16170 (BS103_16155)	pilO	3357826..3358566 (-)	741	WP_000081156.1	type 4a pilus biogenesis protein PilO	Machinery gene
BS103_RS16175 (BS103_16160)	pilN	3358563..3359204 (-)	642	WP_000201224.1	PilN domain-containing protein	Machinery gene
BS103_RS16180 (BS103_16165)	pilM	3359204..3360262 (-)	1059	WP_002014066.1	pilus assembly protein PilM	Machinery gene
BS103_RS16185 (BS103_16170)	ponA	3360426..3362981 (+)	2556	WP_000736677.1	penicillin-binding protein PBP1a	-
BS103_RS16190 (BS103_16175)	-	3362999..3363823 (-)	825	WP_002125608.1	putative RNA methyltransferase	-
BS103_RS16195 (BS103_16180)	-	3363826..3364158 (-)	333	WP_000169599.1	DUF7709 family protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 39032.59 Da
• Isoelectric Point: 4.4618

>NTDB_id=208135 BS103_RS16180 WP_002014066.1 3359204..3360262(-) (pilM) [Acinetobacter baumannii strain A1296]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTTHAAI
AVPTSTVIHKTIEMDADMSDDEREVQIRVDAEQYIPFPLDEVSLDFEVLPDRLANPNRVNVLLVATRTENVETRVEVLEL
ADLNPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
ASRAKKDRSLPDDYEIEVLDPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=208135 BS103_RS16180 WP_002014066.1 3359204..3360262(-) (pilM) [Acinetobacter baumannii strain A1296]
GTGCTCAGGTTATATCGTAAACCTAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTCAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTGATGCCTTTACCCGAAAACAGTGTAGTTGAAAAAAATA
TCTTAAATCCAGAAGCAGTTGCAGAGGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCACTCATGCTGCAATT
GCTGTTCCGACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAGCGATGACGAACGCGAAGTTCAGAT
TCGTGTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTGCCGGATCGTCTTG
CGAATCCAAATCGTGTAAATGTGCTCTTGGTCGCCACAAGAACAGAAAACGTTGAAACACGCGTTGAAGTGCTTGAATTG
GCAGATTTAAATCCTAAATTGGCTGATGTCGAAAGTTACGCGGTTGAGCGCGCTTTTAGCGTGTTTGCTGATAGCTTACC
GATGGGTGCAAATACCATAGGGATTTTAGATATCGGTCATACCATGACGACATTATCTGTCATGCAAAATGGCAAGATTA
TTTATACACGAGAGCAGGTTTTCGGCGGAAAACAACTTACGCTTGAAATTCAAAGTCGTTATGGTTTGTCTTTAGAAGAA
GCAAGCCGAGCAAAAAAAGATCGCTCTTTACCAGATGATTATGAAATTGAAGTGCTAGACCCATTTCTAGATGCGGTAGT
TCAGCAGGCGGCCCGTTCACTACAATTTTTCTTTTCTTCATCCCAATTTAACGAAATAGACCATATTTTGCTCGCTGGTG
GAAATGCGAATATTCCAGGCCTTGCTAAGCTTTTGCAGCAAAAATTGGGCTACCGTGTCACGATTGCCAACCCGTTTTTA
CAAATGGGCTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCTTCATCTTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

3D structure

• AlphaFold DB: N9LI98

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	100	100	1
comM	Acinetobacter nosocomialis M2	99.148	100	0.991
comM	Acinetobacter baylyi ADP1	81.534	100	0.815
pilM	Legionella pneumophila strain ERS1305867	42.09	100	0.423